Development of enzyme-linked immunosorbent assay for Δ12-prostaglandin J2 and its application to the measurement of the endogenous product generated by cultured adipocytes during the maturation phase

Activation of adipogenesis by lipocalin-type prostaglandin D synthase-generated Δ12-PGJ2 acting through PPARγ-dependent and independent pathways

“…Here, we identified that the dominant PGD 2 metabolite in the differentiated adipocytes was Δ 12 -PGJ 2 (Fig. 2B), in good agreement with recent results showing that Δ 12 -PGJ 2 is produced in adipocytes and activated the adipogenic gene expression in 3T3-L1 cells (Hossain et al, 2011). Δ 12 -PGJ 2 enhanced the expression of the adipogenic (Fig.…”

Stimulation of fat storage by prostacyclin and selective agonists of prostanoid IP receptor during the maturation phase of cultured adipocytes

“…Hence, the present study focused on the specific action of prostacyclin and its specific agonists or antagonist for the IP receptor on cultured adipocytes during the maturation phase. Our previous studies have also revealed that cultured 3T3-L1 adipocytes are also capable of generating other proadipogenic prostanoids such as PGD 2 and PGJ 2 derivatives during the maturation phase, which are involved in the stimulation of adipogensis (Mazid et al 2006;Hossain et al 2011). As expected, we found that the treatment of cultured cells with each of aspirin and indomethacin, well-known COX inhibitors, resulted in a significant suppression of adipogenesis during the maturation phase.…”

“…Effect of prostacyclin and selective agonists or antagonist for IP receptor on the storage of fats attenuated by aspirin during the maturation phase of adipocytes Our previous studies have shown that cultured adipocytes during the maturation phase are able to increasingly biosynthesize pro-adipogenic prostanoids, such as PGD 2 , PGJ 2 derivatives, and PGI 2 (Mazid et al 2006;Hossain et al 2011;Rahman et al 2014). The treatment of cultured adipocytes with 500 lM aspirin, a well-known inhibitor for COX enzymes, during the maturation phase resulted in a significant reduction in the generation of the accumulation of triacylglycerols (Fig.…”

“…On the other hand, our previous studies have reported the upregulation of the endogenous synthesis of pro-adipogenic prostanoids, such as PGJ 2 derivatives including 15d-PGJ 2 (Mazid et al 2006) and D 12 -PGJ 2 (Hossain et al 2011), by the non-enzymatic dehydration of PGD 2 in cultured 3T3-L1 cells during the maturation phase. We also confirmed that each of 15d-PGJ 2 and D 12 -PGJ 2 added to the maturation medium was able to rescue the inhibitory effect of COX inhibitors on adipogenesis (Mazid et al 2006;Hossain et al 2011). These findings indicate the autocrine role for those PGJ 2 derivatives in the promotion of adipogenesis during the maturation phase.…”

Prostaglandin J2 series induces the gene expression of monocyte chemoattractant protein-1 during the maturation phase of cultured adipocytes

“…Earlier, cultured 3T3-L1 adipocytes have been shown to express preferentially lipocalin-type PGD synthase (L-PGDS) during the maturation phase (Jowsey et al, 2003;Lu et al, 2004). Recently, we have reported endogenous synthesis of Δ 12 -PGJ 2 and 15d-PGJ 2 by our developed immunological assays specific for each of PGJ 2 series (Mazid et al, 2006;Hossain et al, 2011).…”