Development of Enzyme Immunoassays for 5,6-, 8,9-,11,12-, And 14,15- Eets and the Corresponding Dhets

Sasaki, D.; Yuan, Ying; Gikas, Katherine; Reddy, M. Muralidhar; Falck, John R.; Nithipatikom, Kasem; Campbell, William B.; Callewaert, Denis M.

doi:10.1007/978-1-4615-0193-0_81

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…The synthesis of homochiral and racemic standards of 5,6-; 8,9-; 11,12-; and 14,15-EET (19)(20)(21), 19-and 20-HETE (22), and 5,6-; 8,9-; 11,12-; and 14,15-DHET (23) was followed by the rapid development of methods for their chromatographic resolution (5), stereochemical analysis (24), ELISA (25), and mass spectral identification and quantification (5). The availability of synthetic metabolites opened the door to wide-ranging interrogations of their biological activities and the characterization of P450 isoform-dependent regio and/or stereo-selective AA metabolism (5).…”

Section: Metabolite Characterization Chemical Synthesis and Enzymologymentioning

confidence: 99%

Thematic Review Series: Living History of Lipids The arachidonic acid monooxygenase: from biochemical curiosity to physiological/pathophysiological significance

Capdevila

Falck

2018

Journal of Lipid Research

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The initial studies of the metabolism of arachidonic acid (AA) by the cytochrome P450 (P450) hemeproteins sought to: ) elucidate the roles for these enzymes in the metabolism of endogenous pools of the FA,) identify the P450 isoforms involved in AA epoxidation and ω/ω-1 hydroxylation, and ) explore the biological activities of their metabolites. These early investigations provided a foundation for subsequent efforts to establish the physiological relevance of the AA monooxygenase and its contributions to the pathophysiology of, for example, cancer, diabetes, hypertension, inflammation, nociception, and vascular disease. This retrospective analyzes the history of some of these efforts, with emphasis on genetic studies that identified roles for the murine and genes in renal and vascular physiology and the pathophysiology of hypertension and cancer. Wide-ranging investigations by laboratories worldwide, including the authors, have established a better appreciation of the enzymology, genetics, and physiologic roles for what is now known as the third branch of the AA cascade. Combined with the development of analytical and pharmacological tools, including robust synthetic agonists and antagonists of the major metabolites, we stand at the threshold of novel therapeutic approaches for the treatment of renal injury, pain, hypertension, and heart disease.

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Section: Metabolite Characterization Chemical Synthesis and Enzymologymentioning

confidence: 99%

Thematic Review Series: Living History of Lipids The arachidonic acid monooxygenase: from biochemical curiosity to physiological/pathophysiological significance

Capdevila

Falck

2018

Journal of Lipid Research

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Soluble Epoxide Hydrolase Regulation of Lipid Mediators Limits Pain

et al. 2020

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The role of lipids in pain signaling is well established and built on decades of knowledge about the pain and inflammation produced by prostaglandin and leukotriene metabolites of cyclooxygenase and lipoxygenase metabolism, respectively. The analgesic properties of other lipid metabolites are more recently coming to light. Lipid metabolites have been observed to act directly at ion channels and G protein-coupled receptors on nociceptive neurons as well as act indirectly at cellular membranes. Cytochrome P450 metabolism of specifically long-chain fatty acids forms epoxide metabolites, the epoxy-fatty acids (EpFA). The biological role of these metabolites has been found to mediate analgesia in several types of pain pathology. EpFA act through a variety of direct and indirect mechanisms to limit pain and inflammation including nuclear receptor agonism, limiting endoplasmic reticulum stress and blocking mitochondrial dysfunction. Small molecule inhibitors of the soluble epoxide hydrolase can stabilize the EpFA in vivo, and this approach has demonstrated relief in preclinical modeled pain pathology. Moreover, the ability to block neuroinflammation extends the potential benefit of targeting soluble epoxide hydrolase to maintain EpFA for neuroprotection in neurodegenerative disease.

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Effects of Kakisu (Persimmon Vinegar) on Plasma Antioxidant Power and Urinary 8-Isoprostane Level

Mure

Takeshita²,

Morioka³

et al. 2007

Jpn. J. Hyg.

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Development of Enzyme Immunoassays for 5,6-, 8,9-,11,12-, And 14,15- Eets and the Corresponding Dhets

Cited by 3 publications

References 6 publications

Thematic Review Series: Living History of Lipids The arachidonic acid monooxygenase: from biochemical curiosity to physiological/pathophysiological significance

Thematic Review Series: Living History of Lipids The arachidonic acid monooxygenase: from biochemical curiosity to physiological/pathophysiological significance

Soluble Epoxide Hydrolase Regulation of Lipid Mediators Limits Pain

Effects of Kakisu (Persimmon Vinegar) on Plasma Antioxidant Power and Urinary 8-Isoprostane Level

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