Development of Enzymatic Reactions in Miniaturized Reactors

Honda, Takeshi; Yamaguchi, Hiroshi; Miyazaki, Masaya

doi:10.1002/9783527800599.ch5

Cited by 6 publications

(6 citation statements)

References 175 publications

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“…However, the production of recombinant enzymes is often expensive, with the enzymes being generally unstable and sensitive to changes in process conditions such as pH, temperature, and substrate concentrations [7]. These limitations can be minimized or even eliminated with enzyme immobilization [8]. Covalent binding of enzymes on coated magnetic nanoparticle carriers provides strong linkages between the enzyme and carrier, enzyme leaching in aqueous media is minimized, and no protein contamination of the product occurs [9].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization

Díaz-Hernández

Gracida

García-Almendárez

et al. 2018

Journal of Nanomaterials

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Cross-linking of magnetic nanoparticles with proteins plays a significant role in the preparation of new materials for biotechnological applications. The aim was the maximization of the magnetic mass attracted and protein loading of magnetic iron oxide nanoparticles coated with chitosan, synthesized in a single step by alkaline precipitation. Chitosan-coated magnetite particles (Fe3O4@Chitosan) were cross-linked to a xylanase and a cellulase (Fe3O4@Chitosan@Proteins), showing a 93% of the magnetic saturation of the magnetite. X-ray diffraction pattern in composites corresponds to magnetite. Thermogravimetry and differential scanning calorimetry showed that 162 mg of chitosan was coating one gram of composite and 12 mg of protein was cross-linked to each gram of magnetic support. Cross-linking between enzymes and Fe3O4@Chitosan was confirmed by infrared spectroscopy with Fourier transform, X-ray energy, and X-ray photoelectron spectroscopy dispersion analysis. From dynamic light scattering, transmission and electron microscopy the average particle size distribution was 230 nm and 430 nm for Fe3O4@Chitosan and Fe3O4@Chitosan@Proteins, showing agglomerates of individual spherical particles, with an average diameter of 8.5 nm and 10.8 nm, respectively. The preparation method plays a key role in determining the particle size and shape, size distribution, surface chemistry, and, therefore, the applications of the superparamagnetic nanoparticles.

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Section: Introductionmentioning

confidence: 99%

Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization

Díaz-Hernández

Gracida

García-Almendárez

et al. 2018

Journal of Nanomaterials

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“…Their applications in degradation reactions using immobilized enzymes are described in Section 4. Recent enzyme immobilization methods for other applications in various fields, including industrial uses, have been described in a recent review [19,20,33].…”

Section: Immobilization Methods For Enzyme-immobilized Reactorsmentioning

confidence: 99%

Bioremediation of Hazardous Pollutants Using Enzyme-Immobilized Reactors

Yamaguchi,

Miyazaki

2024

Molecules

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Bioremediation uses the degradation abilities of microorganisms and other organisms to remove harmful pollutants that pollute the natural environment, helping return it to a natural state that is free of harmful substances. Organism-derived enzymes can degrade and eliminate a variety of pollutants and transform them into non-toxic forms; as such, they are expected to be used in bioremediation. However, since enzymes are proteins, the low operational stability and catalytic efficiency of free enzyme-based degradation systems need improvement. Enzyme immobilization methods are often used to overcome these challenges. Several enzyme immobilization methods have been applied to improve operational stability and reduce remediation costs. Herein, we review recent advancements in immobilized enzymes for bioremediation and summarize the methods for preparing immobilized enzymes for use as catalysts and in pollutant degradation systems. Additionally, the advantages, limitations, and future perspectives of immobilized enzymes in bioremediation are discussed.

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“…The opportunities and challenges for carrying out biocatalysis in microfluidic reactors have been previously reviewed [15]. Microreactors are often classified as either chip-type or microtube (microcapillary) devices [16,17]. Chip-type reactors usually have either a bankcard or microscope slide footprint with external dimensions not exceeding a few centimeters [18,19].…”

Section: Biocatalysis In Micro-and Meso-reactors: Types and Definitionmentioning

confidence: 99%

Flow Bioreactors as Complementary Tools for Biocatalytic Process Intensification

Tamborini

Fernandes

Paradisi

et al. 2018

Trends in Biotechnology

270

244

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Biocatalysis has widened its scope and relevance since new molecular tools, including improved expression systems for proteins, protein and metabolic engineering, and rational techniques for immobilization, have become available. However, applications are still sometimes hampered by low productivity and difficulties in scaling up. A practical and reasonable step to improve the performances of biocatalysts (including both enzymes and whole-cell systems) is to use them in flow reactors. This review describes the state of the art on the design and use of biocatalysis in flow reactors. The encouraging successes of this enabling technology are critically discussed, highlighting new opportunities, problems to be solved and technological advances.

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Development of Enzymatic Reactions in Miniaturized Reactors

Cited by 6 publications

References 175 publications

Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization

Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization

Bioremediation of Hazardous Pollutants Using Enzyme-Immobilized Reactors

Flow Bioreactors as Complementary Tools for Biocatalytic Process Intensification

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