Development of embryos reconstructed by interspecies nuclear transfer of adult fibroblasts between buffalo (Bubalus bubalis) and cattle (Bos indicus)

Lu, Fenghua; Shi, Dongquan; Wei, Jingwei; Yang, Sufang; Wei, Yanfei

doi:10.1016/j.theriogenology.2005.03.005

Cited by 51 publications

(47 citation statements)

References 30 publications

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“…Moreover, some embryos were able to form compacting morula on day 6 with large active polygonal nucleoli. Donor cell culture effects on the preimplantation development of SCNT embryos might be even more profound in the case of iSCNT embryos and explains the difference in developmental competence of buffalo-bovine iSCNT embryos in our experiments and other laboratories (Kitiyanant et al 2001, Saikhun et al 2002, Lu et al 2005. Cattle-water buffalo hybrid IVF BLs serve as another confirmation of nuclear-cytoplasm compatibility between these species (Kochhar et al 2002).…”

Section: Discussionsupporting

confidence: 54%

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

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The most successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos has been achieved in closely related species. The analyses of embryonic gene activity in iSCNT embryos of different species combinations have revealed the existence of significant aberrations in expression of housekeeping genes and genes dependent on the major embryonic genome activation (EGA). However, there are many studies with successful blastocyst (BL) development of iSCNT embryos derived from donor cells and oocytes of animal species with distant taxonomical relations (inter-family/inter-class) that should indicate proper EGA at least in terms of RNA polymerase I activation, nucleoli formation, and activation of genes engaged in morula and BL formation. We investigated the ability of bovine, porcine, and rabbit oocytes to activate embryonic nucleoli formation in the nuclei of somatic cells of different mammalian species. In iSCNT embryos, nucleoli precursor bodies originate from the oocyte, while most proteins engaged in the formation of mature nucleoli should be transcribed from genes de novo in the donor nucleus at the time of EGA. Thus, the success of nucleoli formation depends on species compatibility of many components of this complex process. We demonstrate that the time and cell stage of nucleoli formation are under the control of recipient ooplasm. Oocytes of the studied species possess different abilities to support nucleoli formation. Formation of nucleoli, which is a complex but small part of the whole process of EGA, is essential but not absolutely sufficient for the development of iSCNT embryos to the morula and BL stages.

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Section: Discussionsupporting

confidence: 54%

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Lagutina

Zakhartchenko²,

Fulka³

et al. 2011

Reproduction

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“…The fertilization medium was Tyrode's medium supplemented with 50 g/ml heparin, 2.5 mM caffeine. All of the media were supplemented with 60 mg/ml penicillin G and 100 mg/ml streptomycin sulfate, then sterilized by passing through a 0.20 mm filter, and stored at 48C to 88C for up to 4 weeks (Lu et al, 2005).…”

Section: Reagents and Mediamentioning

confidence: 99%

The mRNA expression of brain-derived neurotrophic factor in oocytes and embryos and its effects on the development of early embryos in cattle

Zhou

Shi

et al. 2008

Animal

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The aims of the study were to measure the mRNA expression of brain-derived neurotrophic factor (BDNF) in bovine oocytes and early embryos derived from in vitro fertilization (IVF), parthenogenetic activation (PA) and nuclear transfer (NT), and to investigate the effects of BDNF on the development of IVF and parthenogenetic embryos. Bovine oocytes matured in vitro for 22 h were in vitro fertilized or parthenogenetic activated. By reverse transcription-PCR and quantitative real-time PCR, we found that germinal vesicle (GV) oocytes, metaphase II (MII) oocytes, 4-cell and 8-cell embryos, morulae, and blastocysts were all shown to express mRNA for BDNF. The mRNA levels for BDNF gene were different in bovine oocytes and IVF embryos at different stages (P , 0.01), with the highest expression in MII oocytes and the lowest expression in 8-cell embryos. The mRNA for BDNF was highly expressed in the PA and IVF blastocysts compared to the NT blastocysts (P , 0.01). Supplementation of culture media with BDNF at the concentration of 40 mg/l caused a significant increase in the rates of in vitro-fertilized blastocyst formation (P , 0.05) and parthenogenetic blastocyst formation (P , 0.05). However, the rate of oocyte cleavage in BDNF groups was not significantly different from that in the BDNF-free control (P . 0.05) after IVF or PA. We have also investigated the effects of BDNF on the growth of granulosa cells, which were used for co-culture of bovine early embryos. The results revealed that supplementation of culture media with 20 mg/l BDNF promoted the growth of granulosa cells (P , 0.01). Taken together, these results provided evidence for the role of neurotrophins in promoting early embryonic development as well as in the growth of granulosa cells by the co-culture system, indicating that BDNF can directly or indirectly promote bovine early embryo development.

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“…An attempt at iSCNT using gaur (Bos gaurus) donor cell and domestic bovine (Bos taurus) recipient oocytes produced several pregnancies and a single offspring [23]. In addition, reconstructed embryos resulting from iSCNT between water buffalo (Bubalus bubalis) and domestic bovine developed to the blastocyst stage [14] but did not result in production of term fetuses or offspring. As epigenetic reprogramming of the donor nucleus from its differentiated status to a totipotent state in the presence of interspecies ooplasm requires an elaborate interplay between the nucleus and the ooplasm [45].…”

Section: Discussionmentioning

confidence: 99%

“…Although 25.9% of hybrid embryos in bovine (Bos taurus, 2n = 60) oocytes exposed to water buffalo (Bubalus bubalis) sperm reached the blastocyst stage [8], attempts failed to obtain hybrid offspring [9,10] with the exception of one rare case [11]. In a similar vein, buffalo-bovine iSCNT embryos demonstrated development to the blastocyst stage [12][13][14]; however, neither pregnancy nor natural delivery was reported.In the process of SCNT, the transfer of donor cell mitochondria with nuclei into recipient oocytes resulted in mitochondrial heteroplasmy. Moreover, mitochondrial DNA (mtDNA) accompanying the donor cell was transmitted to offspring at varying but low Journal of Reproduction and Development, Vol.…”

mentioning

confidence: 99%

Influence of Intergeneric/Interspecies Mitochondrial Injection; Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells

Takeda

Srirattana

Matsukawa

et al. 2012

Journal of Reproduction and Development

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Abstract. Interspecies/intergeneric mitochondrial heteroplasmy can occur in interspecies/intergeneric hybrid embryos or following nuclear transfer. In the present study, intergeneric buffalo (Bubalus bubalis) mitochondria (WB-mt) or interspecies murine (Mus spretus) mitochondria (M-mt) were injected into bovine (Bos taurus) oocytes, and the subsequent embryonic development was characterized. Fibroblast mitochondria (WB-mt or M-mt) were microinjected into in vitro matured bovine oocytes followed by oocyte activation by a combination of electrical stimulation and 6-dimethylaminopurine treatment. After seven days of culture, embryo development was evaluated. The copy number of specific mtDNA populations (introduced and native mtDNA) from heteroplasmic oocytes was estimated using real-time PCR. The results illustrated that oocytes injected with either WB-mt or M-mt can develop to the blastocyst stage (20.6% and 19.6%). Cleavage division rates and development to the morula stage in oocytes injected with WB-mt were lower (76.2% and 45.9%, respectively) in comparison with uninjected oocytes (89.2% and 59.1%, respectively) (P<0.05). However, no differences were found in comparing M-mt injected oocytes and controls (P>0.05). An increase in bovine mtDNA copy number was observed at the expanded blastocyst stage of injected embryos (P<0.01), while the number of injected mtDNA was stable throughout development. This study demonstrates that interspecies/intergeneric mitochondrial injected bovine oocytes have the ability to develop to the blastocyst stage after parthenogenetic activation and that injected mtDNA was neither selectively destroyed nor enhanced through development. Moreover, injected intergeneric mitochondria had a demonstrated influence on bovine parthenogenetic development and mtDNA replication. Key words: Bovine oocyte, Mitochondria, Mouse, mtDNA, Water buffalo (J. Reprod. Dev. 58: [323][324][325][326][327][328][329] 2012) S omatic cell nuclear transfer (SCNT) offers the possibility of preserving endangered species, and SCNT has been applied to many mammalian species [1]. However, owing to the limited availability of oocytes from wild animals, the cloning of endangered species benefits from the use of recipient oocytes from a related domestic species. However, this methodology results in the production of nuclear-cytoplasmic hybrids [2,3]. Despite numerous attempts in a wide variety of species, the number of live births of cloned offspring is still limited in instances that combine genetic compartments of closely related species [4,5]. The generation of animals through interspecies/intergeneric SCNT (iSCNT) poses several problems including mitochondrial/genomic DNA incompatibility, embryonic genome activation of the donor nucleus by the recipient oocyte, and availability of suitable foster mothers for iSCNT embryos. The species-specific nature of mitochondrial biogenesis and function makes this modeling particularly significant for iSCNT.F1 hybrids and iSCNT live offspring obtained using river buffalo (Bubal...

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Development of embryos reconstructed by interspecies nuclear transfer of adult fibroblasts between buffalo (Bubalus bubalis) and cattle (Bos indicus)

Cited by 51 publications

References 30 publications

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species

The mRNA expression of brain-derived neurotrophic factor in oocytes and embryos and its effects on the development of early embryos in cattle

Influence of Intergeneric/Interspecies Mitochondrial Injection; Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells

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