Development of embryonic chick oviducts in organ culture under the influences of steroid hormones

Abstract

Explanted oviducts from 13- and 16-day embryonic female chicks responded to estradiol (1 to 4 micrograms/ml) in the culture medium by forming a thickened epithelium folded into crypts and containing goblet cells and by development of the stroma. In medium containing 3 micrograms/ml estradiol and 0.125 micrograms/ml hydrocortisone, 13-day embryonic oviducts exhibited further development: The columnar epithelial cells formed cilia and tubular glands were formed.

Histomorphometric and Progesterone Receptor Immunohistochemical Analysis in the Oviduct of Newly Hatched Chicks Treated with Follicle‐Stimulating Hormone during Embryonic Development

“…The increase in epithelial area is due to the formation of a pseudostratified epithelium, which is related to the increase in the density of nuclei per unitarea and in epithelium thickness. These morphological changes are similar to those induced by 17p-estradiol Kiell et al, 1982).…”

“…Interestingly, histological changes in oviduct magnum mucosa of FSH treated animals such as: luminal epithelial cell proliferation, evaginations from epithelial cells into subepithelial stroma from which tubular glands will be formed and ciliogenesis in epithelial cells are similar to those reported during normal magnum development (Pageaux et al, 1986) and after estradiol treatment O'Malley et al, 1969;Palmiter et al, 1978;Hoisvieux-Ulrich et al, 1982;Kiell et al, 1982).…”