Development of elite rice restorer lines in the genetic background of R022 possessing tolerance to brown planthopper, stem borer, leaf folder and herbicide through marker-assisted breeding

Wan, Biao; Zha, Zhongping; Li, Jinbo; Mao, Xia; Du, Xinying; Lin, Yongjun; Yin, Desuo

doi:10.1007/s10681-013-0988-x

Cited by 20 publications

(13 citation statements)

References 36 publications

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“…Naidoo et al (2012) observed that when transferring lpa1-1 to a maize inbred line, the restoration rate of the genetic background in BC 2 F 1 plants reached 84% using marker-assisted screening of the genomic background. Similar findings were reported for rice regarding resistance to Pyricularia oryzae (84% in BC 3 ; Gouda et al, 2013), tolerance to planthoppers, stem borers, leaf folders, and herbicides (95.8% in BC 3 F 4 ; Wan et al, 2014), and the breeding of pepper varieties rich in capsaicin ester (96% in BC 2 F 1 ; Jeong et al, 2015). In the current study, before the background analyses in background populations, genetic background of 01-20 and D134 were determined.…”

Section: Discussionsupporting

confidence: 77%

Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection

et al. 2017

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Cabbage is an economically important vegetable worldwide. Cabbage Fusarium Wilt (CFW) is a destructive disease that results in considerable yield and quality losses in cole crops. The use of CFW-resistant varieties is the most effective strategy to mitigate the effects of CFW. 01-20 is an elite cabbage line with desirable traits and a high combining ability, but it is highly susceptible to CFW. To rapidly transfer a CFW resistance gene into 01-20 plants, we used microspore cultures to develop 230 doubled haploid (DH) lines from a cross between 01-20 (highly susceptible) and 96-100 (highly resistant). One of the generated DH lines (i.e., D134) was highly resistant to CFW and exhibited a phenotypic performance that was similar to that of line 01-20. Therefore, D134 was applied as the resistance donor parent. We generated 24 insertion–deletion markers using whole genome resequencing data for lines 01-20 and 96-100 to analyze the genomic backgrounds of backcross (BC) progenies. Based on the CFW resistance gene FOC1, a simple sequence repeat (SSR) marker (i.e., Frg13) was developed for foreground selections. We screened 240 BC1 individuals and 280 BC2 individuals with these markers and assessed their phenotypic performance. The proportion of recurrent parent genome (PRPG) of the best individuals in BC1 and BC2 were 95.8 and 99.1%. Finally, a best individual designated as YR01-20 was identified from 80 BC2F1 individuals, with homozygous FOC1 allele and genomic background and phenotype almost the same as those of 01-20. Our results may provide a rapid and efficient way of improving elite lines through the combined application of microspore culture, whole-genome background analysis, and disease resistance-specific marker selection. Additionally, the cabbage lines developed in this study represent elite materials useful for the breeding of new CFW-resistant cabbage varieties.

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Section: Discussionsupporting

confidence: 77%

Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection

et al. 2017

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“…The understanding of the molecular basis of resistance to BPH was greatly advanced with the cloning and characterization of several BPH resistance genes. Among these, a broad spectrum BPH32 gene originally derived from PTB33 and RH has been extensively studied and introgressed into numerous commercial rice varieties with desirable backgrounds [6] [8]. New rice varieties with BPH resistance have been developed and introduced to farmers [1].…”

Section: Discussionmentioning

confidence: 99%

“…The chromosomal location of BPH3 from RH and PTB33 has been reported on the short arm of chromosome 6 [4]. The BPH resistance locus has been widely used in BPH resistance breeding via marker-assisted selection (MAS) [5] [6] [7] [8]. Recently, the BPH3 gene was identified and renamed as BPH32 [9] to avoid confusion with the cloned BPH3 cluster genes located on the short arm of chromosome 4 [10].…”

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confidence: 99%

Loss of Resistance to <i>Nilaparvata lugens</i> May Be Due to the Low-Level Expression of <i>BPH32 </i>in Rice Panicles at the Heading Stage

Jairin¹,

Leelagud²,

Saejueng³

et al. 2017

AJPS

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The brown planthopper (BPH), Nilaparvata lugens (Stål), is the most important insect pest of rice in Asia. Host plant resistance is one of the strategies currently used to control BPH. The resistant rice cultivar Rathu Heenati (RH) carrying the BPH3 gene (recently renamed as "BPH32") remains effective despite more than 30 years of deployment. RH has been determined to be resistant against BPH at all growth stages. However, we observed that BPH could feed on panicles but not on the leaf sheaths of RH. The resistance gene BPH32 was introduced into KDML105 through marker-assisted selection, and the introgression line UBN03078 was developed. This rice line was used to observe the patterns of target gene's regulation. A low-level expression of BPH32 on panicles has been hypothesized to cause susceptibility in UBN03078 at the heading stage. Findings from our gene expression analysis support the hypothesis that the resistance gene was down regulated in the uppermost internodes compared with the leaf sheaths of the heading rice plant. This phenomenon may allow BPH to feed on the panicles of the resistant plants, but this requires further investigation.

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“…Although the use of Bt toxins is a common method for the biological control of pests, these compounds are ineffective against Hemipteran pests. Pyramiding multiple genes, such as BPH14, BPH15, and Cry1C in elite restorer lines enhanced resistance to BPH, SSB, and RLF (Wan et al 2014). A fusion protein consisting of the DI and DII domains of Bt Cry1Ac and the carbohydrate-binding domain of garlic lectin showed remarkable toxicity against Lepidopteran and Hemipteran insects (Boddupally et al 2018).…”

Section: Bt and Lectins Toxinsmentioning

confidence: 99%

Current understanding of the genomic, genetic, and molecular control of insect resistance in rice

Chen

Guo

et al. 2020

Mol Breeding

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Rice (Oryza sativa) is both a vital source of food and a key model cereal for genomic research. Insect pests are major factors constraining rice production. Here, we provide an overview of recent progress in functional genomics research and the genetic improvements of insect resistance in rice. To date, many insect resistance genes have been identified in rice, and 14 such genes have been cloned via a map-based cloning approach. The proteins encoded by these genes perceive the effectors of insect and activate the defense pathways, including the expression of defense-related genes, including mitogen-activated protein kinase, plant hormone, and transcription factors; and defense mechanism against insects, including callose deposition, trypsin proteinase inhibitors (TryPIs), secondary metabolites, and green leaf volatiles (GLVs). These ongoing functional genomic studies provide insights into the molecular basis of rice-insect interactions and facilitate the development of novel insect-resistant rice varieties, improving long-term control of insect pests in this crucial crop.

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Development of elite rice restorer lines in the genetic background of R022 possessing tolerance to brown planthopper, stem borer, leaf folder and herbicide through marker-assisted breeding

Cited by 20 publications

References 36 publications

Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection

Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection

Loss of Resistance to <i>Nilaparvata lugens</i> May Be Due to the Low-Level Expression of <i>BPH32 </i>in Rice Panicles at the Heading Stage

Current understanding of the genomic, genetic, and molecular control of insect resistance in rice

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Development of elite rice restorer lines in the genetic background of R022 possessing tolerance to brown planthopper, stem borer, leaf folder and herbicide through marker-assisted breeding

Cited by 20 publications

References 36 publications

Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection

Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection

Loss of Resistance to &lt;i&gt;Nilaparvata lugens&lt;/i&gt; May Be Due to the Low-Level Expression of &lt;i&gt;BPH32 &lt;/i&gt;in Rice Panicles at the Heading Stage

Current understanding of the genomic, genetic, and molecular control of insect resistance in rice

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Loss of Resistance to <i>Nilaparvata lugens</i> May Be Due to the Low-Level Expression of <i>BPH32 </i>in Rice Panicles at the Heading Stage