Development of efficient Agrobacterium rhizogenes-mediated hairy root system in Curcuma longa L. and elicitation driven enhanced production of pharmaceutically important curcuminoids

Sandhya, S.; Giri, Archana

doi:10.1007/s11627-022-10298-1

Cited by 12 publications

(3 citation statements)

References 62 publications

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“…Acetosyringone is commonly used to increase the frequency of transfer and reduce the time required to induce root hairs in different plants. The appropriate concentration of acetosyringone is one of the important factors for increasing the e ciency of gene transfer by R. rhizogenes (Sandhya and Giri 2022). In our study, the effect of different concentrations of acetosyringone on the rooting rate of strain ATCC 15834 in quinoa was investigated, and the greatest effect on root biomass was obtained at 1 mM acetosyringone concentration.…”

Section: Discussionmentioning

confidence: 89%

Effect of Acetosyringone, Sucrose and Nutrients on Transgenic Hairy Root Induction in Chenopodium quinoa using different Rhizobium rhizogenesstrains

Massah

Rabiei

2023

Preprint

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Quinoa is a plant with special nutritional and biological properties and tolerant in strict environmental conditions, which can be considered to a strategic product for the future food supply of mankind, as a pseudocereal. Quinoa root is rich in bioactive compounds with antimicrobial, antioxidant activity and diverse medicinal and nutritional properties that can have a potential for commercialization. The use of cultivation and propagation techniques in controlled laboratory conditions, such as the induction of differentiated tissues of hairy roots, allows the production of plants and the rapid and massive access to secondary metabolites. In this research, the induction of hairy roots was evaluated using four Rhizobium rhizogenes strains, including A4, A7, 11325, and ATCC 15834, in quinoa and the effect of different concentrations of acetosyringone and sucrose and removal of macro elements in the culture medium in increasing the rooting performance of R. rhizogenes was investigated. The results showed the different effects of the mutual effect of different concentrations of sucrose and acetosyringone, as well as the commonly used elements of the half strength MS culture medium on the biomass of hairy roots. The most optimal conditions for the induction of hairy roots culture were obtained in half strength MS culture medium, containing 30 g/l sucrose and 1 mM acetosyringone and without NH4NO3 salt. This efficient protocol provides a basis for future studies to be able to use the induced hairy root as a source of valuable secondary metabolites of quinoa root and create a new approach for exploiting the nutritional, medicinal and health compounds of the quinoa plant.

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Section: Discussionmentioning

confidence: 89%

Effect of Acetosyringone, Sucrose and Nutrients on Transgenic Hairy Root Induction in Chenopodium quinoa using different Rhizobium rhizogenesstrains

Massah

Rabiei

2023

Preprint

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“…Similarly, studies have reported that the 21st day of elicitation at the end of the exponential phase of Eclipta prostrata hairy roots was found to be the ideal time for achieving the greatest biomass and caffeoylquinic acid derivative production (Maciel et al 2022). The study by Sandhya and Giri (2022) revealed that the 24th day-old hairy root of Curcuma longa, treated with an elicitor at the end of the exponential phase, produced the maximum amount of curcuminoids. In another study, Mahendran and Vimolmangkang (2024) described that the greatest hairy root biomass and friedelin and epifriedelanol contents were found after 28 days, which was the ideal time for elicitation in C. sativa.…”

Section: Discussionmentioning

confidence: 99%

Elicitation boosts withaferin A and withanolide A production in Withania somnifera (L.) Dunal hairy root cultures

Mahendran,

Vimolmangkang

2024

Preprint

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Withania somnifera (L.) Dunal, an Ayurvedic herb rich in withanosides, has anti-cancer and health-rejuvenating properties. These bioactive chemicals, primarily found in the roots, are of significant interest for production in hairy root cultures. In this study, researchers investigated a hairy root line produced by Rhizobium rhizogenes (ATCC 43057), varying cell density, infection period, and acetosyringone concentrations. The most successful root induction frequency (31.66% ± 2.98%) occurred at a bacterial cell density of OD600 = 0.8 combined with a 20-min infection period and 200 µM acetosyringone. After 25 days, the hairy root culture achieved its highest biomass (5.20 ± 0.15 g/50 mL flask fresh weight (FW)) in MS liquid medium with 3% sucrose. At 30 and 25 days, the greatest amounts of withanolide A (0.824 ± 0.01 mg/g DW) and withaferin A (1.292 ± 0.02 mg/g DW) were observed. Hairy roots were further treated with methyl jasmonate (MJ) and salicylic acid (SA) at various concentrations (25, 50, and 100 µM). Notably, the maximum content of withanolide A (2.90 ± 0.08 mg/g DW, 3.86-fold) and withaferin A (3.40 ± 0.01 mg/g DW, 2.61-fold) was obtained in the hairy roots treated with MJ at 100 µM. After 48 h, the content of withanolide A reached 3.16 ± 0.03 mg/g DW (6.72-fold), and withaferin A peaked at 4.32 ± 0.05 mg/g DW (4.59-fold) when treated with 50 µM SA + 100 µM MJ. This study demonstrated that W. somnifera hairy root culture enhances large-scale production of withanolide A and withaferin A.

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“…Using A4, A4T, and A8196 strains of Agrobacterium rhizogenes hairy root phenotype of Curcuma longa roots was produced with cultures transformed with the A4 strain of Agrobacterium rhizogenes showed maximum biomass production. This work used an elicitor like methyl-jasmonate and produced three curcuminoids, namely curcumin, demethoxycurcumin, and bisdemethoxycurcumin, with notable outcomes ( Sandhya & Giri, 2022 ). Moreover, yeast extract and salicylic acid also elicit enhanced curcumin biosynthesis in in-vitro cultures ( Lan et al, 2019 ).…”

Section: Biotechnological and Metabolic Engineering Used To Enhance A...mentioning

confidence: 99%

Biosynthesis of anticancer phytochemical compounds and their chemistry

Dogra

Kumar

2023

Front. Pharmacol.

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Cancer is a severe health issue, and cancer cases are rising yearly. New anticancer drugs have been developed as our understanding of the molecular mechanisms behind diverse solid tumors, and metastatic malignancies have increased. Plant-derived phytochemical compounds target different oncogenes, tumor suppressor genes, protein channels, immune cells, protein channels, and pumps, which have attracted much attention for treating cancer in preclinical studies. Despite the anticancer capabilities of these phytochemical compounds, systemic toxicity, medication resistance, and limited absorption remain more significant obstacles in clinical trials. Therefore, drug combinations of new phytochemical compounds, phytonanomedicine, semi-synthetic, and synthetic analogs should be considered to supplement the existing cancer therapies. It is also crucial to consider different strategies for increased production of phytochemical bioactive substances. The primary goal of this review is to highlight several bioactive anticancer phytochemical compounds found in plants, preclinical research, their synthetic and semi-synthetic analogs, and clinical trials. Additionally, biotechnological and metabolic engineering strategies are explored to enhance the production of bioactive phytochemical compounds. Ligands and their interactions with their putative targets are also explored through molecular docking studies. Therefore, emphasis is given to gathering comprehensive data regarding modern biotechnology, metabolic engineering, molecular biology, and in silico tools.

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Development of efficient Agrobacterium rhizogenes-mediated hairy root system in Curcuma longa L. and elicitation driven enhanced production of pharmaceutically important curcuminoids

Cited by 12 publications

References 62 publications

Effect of Acetosyringone, Sucrose and Nutrients on Transgenic Hairy Root Induction in Chenopodium quinoa using different Rhizobium rhizogenesstrains

Effect of Acetosyringone, Sucrose and Nutrients on Transgenic Hairy Root Induction in Chenopodium quinoa using different Rhizobium rhizogenesstrains

Elicitation boosts withaferin A and withanolide A production in Withania somnifera (L.) Dunal hairy root cultures

Biosynthesis of anticancer phytochemical compounds and their chemistry

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