Development of dried serum spot sampling techniques for the assessment of trace elements in serum samples by LA-ICP-MS

Chantada-Vázquez, María Del Pilar; Moreda–Piñeiro, Jorge; Cantarero-Roldán, Alicia M.; Bermejo–Barrera, Pilar; Moreda–Piñeiro, Antonio

doi:10.1016/j.talanta.2018.04.049

Cited by 18 publications

(10 citation statements)

References 26 publications

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“…Moreda‐Piñeiro et al reported iodine determination in DBS using matrix‐matched calibration by LA‐ICP‐MS . Chantada‐Vázquez et al analyzed the trace elements in DBS using matrix‐matched calibration by LA‐ICP‐MS …”

Section: Introductionmentioning

confidence: 73%

“…The basic concept of dried‐droplet LA consists of depositing a well‐defined volume of liquid sample on a solid support, evaporating the solvent, and examining the remaining dried residue by means of laser ablation coupled with inductively coupled plasma mass spectrometry (LA‐ICP‐MS) . LA‐ICP‐MS is commonly used for analyzing precious natural specimens and has been applied successfully to the determination of geological and biological samples . However, the lack of a series of appropriate certified reference materials (CRMs) with the same or similar matrix composition limits the applicability of this approach.…”

Section: Introductionmentioning

confidence: 99%

“…Matrix difference can cause elemental fractionation so that the abundances of the ions detected after m/z separations are often not entirely representative of the composition of the original sample . In order to overcome this problem, on‐line solution‐based calibration and self‐made matrix‐matched standards were proposed . Some elements can easily bind to proteins, but the majority of bromine and chlorine is present in the free form.…”

Section: Introductionmentioning

confidence: 99%

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Direct analysis of bromine and iodine in dried serum spots by laser ablation inductively coupled plasma mass spectrometry

Han

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale: Accurate quantitative analysis of bromine and iodine in serum is an important aspect of monitoring body condition, but the volatile loss of halogen in sample pretreatment is a troublesome problem. We present a validated and flexible high-throughput method for quantification of bromine and iodine in dried serum spots (DSS) using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) and an external aqueous standard calibration curve. The influence of serum matrix and laser ablation (LA) conditions on the analysis of bromine and iodine in DSS was researched systematically.Methods: Aqueous standards without matrix matching were used for calibration to analyze bromine and iodine in serum by LA-ICP-MS. 5-μL volumes of the aqueous standard solution and serum samples in 10 times diluted concentration were deposited on the PTFE paper to form dried standard calibration spots (DSCS) and DSS, of less than 2 mm in diameter. LA was performed using a focused Nd:YAG laser beam in raster lineal scan mode. Results:The limits of detection (LODs) for bromine and iodine in DSS were 0.23 and 0.03 mg L −1 , respectively. The relative standard deviation (RSD) for this method was less than 10%. The samples were also detected with matrix matching calibration by ICP-MS. The accuracy of the method was verified by statistical analysis of these results from ICP-MS and LA-ICP-MS. The accuracy is satisfactory with recoveries ranging from 81.5% to 118%. Conclusions:A novel and simple approach for high-throughput screening of bromine and iodine in DSS has been established by LA-ICP-MS. Calibration could be achieved using an aqueous standard solution instead of a matrix-matching solution. The method allowed analysis of low-volume biological samples without derivatization and decreased the risk of contamination or loss.

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Section: Introductionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Direct analysis of bromine and iodine in dried serum spots by laser ablation inductively coupled plasma mass spectrometry

Han

et al. 2020

Rapid Comm Mass Spectrometry

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“…Direct biofluid analysis by LA-ICP-MS is also a topic being continuously explored. A recent approach for serum analysis by LA-ICP-MS using dried serum spot (DSS) sampling, using only 20 μL of serum, was proposed by Chantada-Vázquez et al [9]. Direct multi-element determination (Al, Be, Ca, Cu, Fe, K, Li, Mg, Mn, Mo, Na, P, Rb, Se, V, and Zn) in DSS sampled in filter paper discs with 2.7 mm diameter and employing acetic acid as an oxidizing agent (used to remove organic matter from the biological matrix) was achieved at low limits of detection levels.…”

Section: Developments In La-icp-ms For Bioanalysismentioning

confidence: 99%

Recent advances in LA-ICP-MS for biomedical applications

Barbosa

Sussulini

2020

BSI

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The applications of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in biomedical research are progressively increasing in the last decade. The main advantage of this powerful analytical technique is the ability to perform multi-elemental analysis directly from biological samples, such as tissues, organs, and single cells, with minimal sample preparation. This feature is particularly important for studies regarding disease biomarkers and mechanism of action of drugs. In this mini-review, the recent advances in the use of LA-ICP-MS for the analysis of biological samples will be discussed, as well as representative biomedical applications.

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“…Other laboratories used LA-ICP-MSI or related techniques to analyze the composition of human kidney stones [54], identify inflammatory hotspots associated with elevated metal quantities in placental tissue from low-birth-weight pregnancy [55], map trace and bulk elements in tumor tissue obtained from an individual previously treated with cisplatin [56], and define contaminations in human hair or teeth resulting from occupational exposure or unfavorable dental fillings [57][58][59]. Likewise, the first studies analyzed element distribution in human eye lenses, brain tissue, or skin biopsies from patients suffering from renal insufficiency [60][61][62], optimized radiodosages in treatment of tumors [63], or determined individual metals or metal-containing contrast agents in human blood or serum [64][65][66][67][68]. In addition, there are many inborn errors of metal and metalloid metabolism human trace elements disorders, in which LA-ICP-MSI offers advantages.…”

Section: Translational Aspects Of La-icp-msimentioning

confidence: 99%

Laser Ablation Inductively Coupled Plasma Spectrometry: Metal Imaging in Experimental and Clinical Wilson Disease

2019

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Wilson disease is an inherited disorder caused by mutations in the ATP7B gene resulting in copper metabolism disturbances. As a consequence, copper accumulates in different organs with most common presentation in liver and brain. Chelating agents that nonspecifically chelate copper, and promote its urinary excretion, or zinc salts interfering with the absorption of copper from the gastrointestinal tract, are current medications. Also gene therapy, restoring ATP7B gene function or trials with bis-choline tetrathiomolybdate (WTX101) removing excess copper from intracellular hepatic copper stores and increasing biliary copper excretion, is promising in reducing body’s copper content. Therapy efficacy is mostly evaluated by testing for evidence of liver disease and neurological symptoms, hepatic synthetic functions, indices of copper metabolisms, urinary copper excretions, or direct copper measurements. However, several studies conducted in patients or Wilson disease models have shown that not only the absolute concentration of copper, but also its spatial distribution within the diseased tissue is relevant for disease severity and outcome. Here we discuss laser ablation inductively coupled plasma spectrometry imaging as a novel method for accurate determination of trace element concentrations with high diagnostic sensitivity, spatial resolution, specificity, and quantification ability in experimental and clinical Wilson disease specimens.

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Development of dried serum spot sampling techniques for the assessment of trace elements in serum samples by LA-ICP-MS

Cited by 18 publications

References 26 publications

Direct analysis of bromine and iodine in dried serum spots by laser ablation inductively coupled plasma mass spectrometry

Direct analysis of bromine and iodine in dried serum spots by laser ablation inductively coupled plasma mass spectrometry

Recent advances in LA-ICP-MS for biomedical applications

Laser Ablation Inductively Coupled Plasma Spectrometry: Metal Imaging in Experimental and Clinical Wilson Disease

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