Development of Double Eastern Blotting for Major Licorice Components, Glycyrrhizin and Liquiritin for Chemical Quality Control of Licorice Using <i>anti</i>-Glycyrrhizin and <i>anti</i>-Liquiritin Monoclonal Antibodies

Fujii, Shunsuke; Morinaga, Osamu; Uto, Takuhiro; Nomura, Shuichi; Shoyama, Yukihiro

doi:10.1021/acs.jafc.5b04732

Cited by 21 publications

(11 citation statements)

References 30 publications

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“…The establishment of a double EB by immunostaining using anti-GA and anti-liquiritin (Liq) MAbs was successfully applied in determining the immunohistochemical distribution and localization of GA and Liq in fresh licorice root. Moreover, its application to identify GA and Liq in licorice and Kampo medicines was also reported [ 87 ].…”

Section: Immunoassay For Mpnp Using Mabsmentioning

confidence: 99%

Monoclonal Antibodies and Immunoassay for Medical Plant-Derived Natural Products: A Review

et al. 2017

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Owing to the widespread application value, monoclonal antibodies (MAbs) have become a tool of increasing importance in modern bioscience research since their emergence. Recently, some researchers have focused on the production of MAbs against medical plant-derived natural products (MPNP), the secondary metabolites of medical plants. At the same time, various immunoassay methods were established on the basis of these MPNP MAbs, and then rapidly developed into a novel technique for medical plant and phytomedicine research in the area of quality control, pharmacological analysis, drug discovery, and so on. Dependent on the research works carried out in recent years, this paper aims to provide a comprehensive review of MAbs against MPNP and the application of various immunoassay methods established on the basis of these MAbs, and conclude with a short section on future prospects and research trends in this area.

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Section: Immunoassay For Mpnp Using Mabsmentioning

confidence: 99%

Monoclonal Antibodies and Immunoassay for Medical Plant-Derived Natural Products: A Review

et al. 2017

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“…Quality control of licorice root is needed to assure uniformity in the concentrations of these compounds, which can differ according to licorice species, growth place, and harvest season, and so on. To this end, we previously prepared MAbs against GC [4] and Liq [16] and developed an eastern blotting system for detection of GC and Liq and their aglycones (glycyrrhetinic acid and liquiritigenin, respectively) [17] in order to make evident the resource of licorice for further researches. In this method, crude licorice extract and/or traditional Chinese medicine extract are developed on a Thin acrosome reaction in sperm.…”

Section: Components Of Licorice and Quantitative And Qualitative Controlmentioning

confidence: 99%

In vitro Fertilization with Mouse Sperm Activated by Components of Licorice Root Extract

Tung¹,

Tanaka²,

Tsujimura³

et al. 2016

Nat Prod Chem Res

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Licorice (Glycyrrhiza species) is an important natural product that is frequently used in traditional Chinese medicine. In this review article, we discuss the quality control of licorice using monoclonal antibodies against the licorice components glyzyrrhizin (GC) and liquiritin (Liq); the monitoring of licorice extract activity using an assay system in mice; and the isolation of two active phenolic components, isolitiritigenin and formononetin, and their effectiveness for improving in vitro fertilization in mice. Finally, we present the outstanding questions and potential applications of licorice root extract.

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“…Modern research studies showed that GRER had extensive pharmacological effects in antispasmodic, antidiabetic, antiosteoporosis, antidepressive, antitussive and expectorant, hepatoprotective, and memory-enhancing [5][6][7][8][9][10][11]. e major bioactive components of GRER were saponins, flavonoid glycosides, and free phenolic compounds [12]. Approximately 250 compounds have been reported from GRER, and more than 151 compounds in GRER have been determined up to present [13].…”

Section: Introductionmentioning

confidence: 99%

Glycyrrhizae Radix et Rhizoma Processed by Sulfur Fumigation Damaged the Chemical Profile Accompanied by Immunosuppression and Liver Injury

Jiang

Xiao

Yan³

et al. 2020

BioMed Research International

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Glycyrrhizae Radix et Rhizoma (GRER) has been used as a medicinal plant and dietary supplements for its beneficial effect in immunomodulatory effects. Sulfur fumigation (SF) processing was widely used in the storage and maintenance of Chinese medicine because of its convenience and cheapness. However, the disadvantage of SF has been reported, but the systematic study of SF on GRER was deficient. In this paper, the active ingredients, sulfur-fumigated products, immunomodulatory effect, and liver injury of SF-GRER were studied. After SF, the liquiritin decreased from 4.49 ± 0.03 mg/g to 3.94 ± 0.08 mg/g (P<0.01). Compared with the NSF-GRER group, the SF-GRER group showed a decreased immunoregulation in the thymus index, spleen index, and serum IL-6 and SOD levels (P<0.05). After 2 weeks of continuous intragastric administration of SF-GRER in healthy mice, the level of serum aspartate aminotransferase (AST) significantly increased (P<0.05) and the area of liver lesion significantly increased compared with the NSF-GRER (P<0.05) group. The sulfonated products (m/z, 631.13) corresponding to liquiritin apioside (m/z, 551.17) and isoliquiritin apioside (m/z, 551.17) were screened out in SF-GRER by using UPLC-Orbitrap-MS. The sulfonated products provided in this paper were discovered for the first time and could be powerfully applied for the identification of SF-GRER. SF destroyed the chemical composition of GRER, inhibited immunoregulation, and induced liver injury. The feasibility of this processing method needs to be reconsidered.

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Development of Double Eastern Blotting for Major Licorice Components, Glycyrrhizin and Liquiritin for Chemical Quality Control of Licorice Using anti-Glycyrrhizin and anti-Liquiritin Monoclonal Antibodies

Cited by 21 publications

References 30 publications

Monoclonal Antibodies and Immunoassay for Medical Plant-Derived Natural Products: A Review

Monoclonal Antibodies and Immunoassay for Medical Plant-Derived Natural Products: A Review

In vitro Fertilization with Mouse Sperm Activated by Components of Licorice Root Extract

Glycyrrhizae Radix et Rhizoma Processed by Sulfur Fumigation Damaged the Chemical Profile Accompanied by Immunosuppression and Liver Injury

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