Development of donor-specific microchimerism in liver transplant recipient with HLA-DRB1 and -DQB1 mismatch related to rejection episodes

Araújo, Margareth Batistella; Leonardi, Luiz Sérgio; Boin, I.F.S.F.; Leonardi, M.I.; Magna, Luís Alberto; Donadi, Eduardo Antônio; Kraemer, Maria Helena Stangler

doi:10.1016/j.transproceed.2004.03.097

Cited by 9 publications

(4 citation statements)

References 9 publications

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“…In organ transplantation, it has been hypothesized that donor cell microchimerism facilitates graft acceptance 25. It has also been suggested that donor cells with HLA more similar to host cells are more likely to result in microchimerism and potential risk for autoimmune diseases 23.…”

Section: Discussionmentioning

confidence: 99%

Combined HLA-DR and -DQ disparity is associated with a stable course of ulcerative colitis after liver transplantation for primary sclerosing cholangitis

et al. 2007

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Combined disparity of human leukocyte antigen (HLA)-DR and -DQ between mother and fetus is associated with less severe ulcerative colitis (UC) during pregnancy. We evaluated whether donor-recipient HLA disparity after liver transplantation (LT) affects UC in patients with primary sclerosing cholangitis (PSC). Sixty-nine consecutive patients with PSC underwent LT; all underwent colonoscopy before LT; 48 had UC before and 3 had de novo UC after LT. Clinical and laboratory data, activity and treatment of UC, post-LT cytomegalovirus infection, and disparity of HLA-A, -B, -DR, and -DQ for each donor-recipient pair were evaluated. Pre-LT quiescent UC was present in 26 patients. Post-LT UC activity was evaluated in 36 of 51 patients with UC who had not undergone pre-LT colectomy and who had Ͼ12 months' post-LT survival. Of these, 16 were stable, 17 had worsened, and 3 had de novo UC. Seven required colectomy (4 for dysplasia or cancer) after LT. Post-LT cytomegalovirus viremia was neither associated with worse UC activity (P ϭ 0.58) nor de novo UC. Disparity with respect to HLA-A, -B, -DR, and -DQ was found in 58%, 27%, 44%, and 39% donor-recipient pairs, respectively. Post-LT UC course was similar with respect to single HLA disparity. However, disparity in none or only one HLA-DR or -DQ was significantly associated with worse activity compared with patients with disparity at both (65% vs. 0%, P ϭ 0.009). Logistic regression found that the disparity for both -DR and -DQ was the only factor statistically significantly associated with post-LT UC activity. We conclude that disparity in both HLA-DR and -DQ between donor and recipient is associated with stable UC activity after LT. Liver Transpl 13: 552-557, 2007.

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Section: Discussionmentioning

confidence: 99%

Combined HLA-DR and -DQ disparity is associated with a stable course of ulcerative colitis after liver transplantation for primary sclerosing cholangitis

et al. 2007

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“…The rejection rate was 25% in the microchimerismpositive recipients and 57% in the microchimerism-negative patients (P < 0.05). Araujo et al [11] recently reported that 71.9% of 32 patients with liver transplant presented positive microchimerism. Among the 23 microchimerism-positive recipients, 16 did not experience rejection and only 7 recipients showed rejection.…”

Section: Clinical Outcomes Of Transplanted Kidneys In Microchimerism-mentioning

confidence: 97%

“…In summary, the detection of donor-DNA from the blood of recipients with renal transplantation was positively associated with the immunotolerance of transplanted organs in the recipients. Although the exact mechanisms by which microchimerisms were formed remain largely unknown [10][11][12][13][14][15], the microchimerism were proposed to be derived from kidney cells, organcontained leukocytes or blood stem cells [15]. From a clinical view, the microchimerism might be one of several immunological mechanisms that lead to long-term graft survival.…”

Section: Clinical Outcomes Of Transplanted Kidneys In Microchimerism-mentioning

confidence: 99%

Presence of donor-and-recipient-derived DNA microchimerism in the cell-free blood samples of renal transplantation recipients associates with the acceptance of transplanted kidneys

Fu¹,

Wang²,

Zhou³

et al. 2006

Asian J Andrology

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“…The exact role of chimeric cells in transplantation is unclear, but previous studies have proposed that the migration of donor-derived cells into recipient tissues induces tolerance in the recipient [12,13]. To further enhance such systemic chimerism, bone marrow transplantation has been used.…”

Section: Introductionmentioning

confidence: 99%

Ingraft chimerism in lung transplantation - a study in a porcine model of obliterative bronchiolitis

et al. 2011

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BackgroundBronchial epithelium is a target of the alloimmune response in lung transplantation, and intact epithelium may protect allografts from rejection and obliterative bronchiolitis (OB). Herein we study the influence of chimerism on bronchial epithelium and OB development in pigs.MethodsA total of 54 immunosuppressed and unimmunosuppressed bronchial allografts were serially obtained 2-90 days after transplantation. Histology (H&E) was assessed and the fluorescence in situ hybridization (FISH) method for Y chromosomes using pig-specific DNA-label was used to detect recipient derived cells in graft epithelium and bronchial wall, and donor cell migration to recipient organs. Ingraft chimerism was studied by using male recipients with female donors, whereas donor cell migration to recipient organs was studied using female recipients with male donors.ResultsEarly appearance of recipient-derived cells in the airway epithelium appeared predictive of epithelial destruction (R = 0.610 - 0.671 and p < 0.05) and of obliteration of the bronchial lumen (R = 0.698 and p < 0.01). All allografts with preserved epithelium showed epithelial chimerism throughout the follow-up. Antirejection medication did not prevent, but delayed the appearance of Y chromosome positive cells in the epithelium (p < 0.05), or bronchial wall (p < 0.05).ConclusionsIn this study we demonstrate that early appearance of Y chromosomes in the airway epithelium predicts features characteristic of OB. Chimerism occurred in all allografts, including those without features of OB. Therefore we suggest that ingraft chimerism may be a mechanism involved in the repair of alloimmune-mediated tissue injury after transplantation.

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Development of donor-specific microchimerism in liver transplant recipient with HLA-DRB1 and -DQB1 mismatch related to rejection episodes

Cited by 9 publications

References 9 publications

Combined HLA-DR and -DQ disparity is associated with a stable course of ulcerative colitis after liver transplantation for primary sclerosing cholangitis

Combined HLA-DR and -DQ disparity is associated with a stable course of ulcerative colitis after liver transplantation for primary sclerosing cholangitis

Presence of donor-and-recipient-derived DNA microchimerism in the cell-free blood samples of renal transplantation recipients associates with the acceptance of transplanted kidneys

Ingraft chimerism in lung transplantation - a study in a porcine model of obliterative bronchiolitis

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