Development of DNA microsatellite markers in the multiband butterflyfish (Chaetodon multicinctus)

Abstract

Twelve polymorphic microsatellite loci were developed in the multiband (pebbled) butterflyfish Chaetodon multicinctus. The loci were scored in 45 individuals from Hawaii. There were five to 21 alleles per locus with observed heterozygosity ranging from 0.419 to 0.883. Four of the primer sets also reliably amplified polymorphic loci in Chaetodon quadrimaculatus. We expect these markers to be useful for studies of genetic population structure and kinship, for example to determine whether new recruits settling onto reefs are related.

Cross‐species amplification of 44 microsatellite loci developed for Chaetodon trifascialis, C. lunulatus and C. vagabundus in 22 related butterflyfish species

“…2009) in each of four individuals of 22 related Chaetodon species was tested. Microsatellite primers designed for Chaetodon multicintus (Heist et al. 2008) were not included because of the restricted geographical range of C. multicintus .…”

“…To provide a microsatellite primer database suitable for Chaetodon species with large geographic ranges, we tested the cross-species transferability of 16 microsatellite primers designed for Chaetodon lunulatus (Lawton et al 2010), 13 microsatellite primers designed for Chaetodon trifascialis (Lawton et al 2010) and 15 microsatellite primers designed for Chaetodon vagabundus (Molecular Ecology Resources Primer Development Consortium et al 2009) in each of four individuals of 22 related Chaetodon species was tested. Microsatellite primers designed for Chaetodon multicintus (Heist et al 2008) were not included because of the restricted geographical range of C. multicintus.…”

“…Consequently, cross-species amplification of previously developed microsatellite markers is often used to examine the population genetic structure of related species for which specific microsatellites have not been developed. Microsatellite markers suitable for the studies of population connectivity have thus far only been developed for four species of butterflyfishes (Heist et al 2008;Molecular Ecology Resources Primer Development Consortium et al 2009;Lawton et al 2010). Crosstesting these loci across a wide range of Chaetodon species co-occurring on the Great Barrier Reef will permit the development of a broad suite of genetic markers with high amplification success rates across multiple species.…”

“…Consequently, cross‐species amplification of previously developed microsatellite markers is often used to examine the population genetic structure of related species for which specific microsatellites have not been developed. Microsatellite markers suitable for the studies of population connectivity have thus far only been developed for four species of butterflyfishes (Heist et al. 2008; Molecular Ecology Resources Primer Development Consortium et al.…”