Development of DNA Microarray Technique to Evaluation of Transcriptional Responses of Wheat to Mycosphaerella graminicolla (septoria tritici)

Arash, Fazeli

doi:10.4172/scientificreports.152

Cited by 1 publication

(1 citation statement)

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“…Wheat defence mechanisms against Z. tritici have been investigated recently by molecular and biochemical studies in order to identify host responses, but previous works focused on incompatible interactions, with compatible ones studied only as a reference. It has been suggested that pathogenesis‐related (PR) proteins, reactive oxygen species (ROS) metabolism, cell wall reinforcement with callose, and signalling via the MAPK pathway are involved in wheat resistance against STB (Shetty et al ., , , ; Adhikari et al ., ; Rudd et al ., ; Diani et al ., ; Fazeli et al ., ; Yang et al ., ). Indeed, most of the studied defence‐related genes were differentially expressed between compatible and incompatible interactions.…”

Section: Introductionmentioning

confidence: 99%

Cultivar‐dependent partial resistance and associated defence mechanisms in wheat against Zymoseptoria tritici

et al. 2017

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Septoria tritici blotch caused by the fungus Zymoseptoria tritici is one of the most devastating foliar diseases of wheat. Knowledge regarding mechanisms involved in resistance against this disease is required to breed durable resistances. This study compared the expression of defence and pathogenicity determinants in three cultivars in semicontrolled culture conditions. The most susceptible cultivar, Alixan, presented higher necrosis and pycnidia density levels than Altigo, the most resistant one. In Premio, a moderately resistant cultivar, necrosis developed as in Alixan, while pycnidia developed as in Altigo. In noninfectious conditions, genes coding for PR1 (pr1), glucanase (gluc) and allene oxide synthase (aos) were constitutively expressed at a higher level in both Altigo and Premio than in Alixan, while chitinase2 (chit2), phenylalanine ammonia-lyase (pal), peroxidase (pox2) and oxalate oxidase (oxo) were expressed at a higher level in Premio only. Except for aos, all genes were induced in Alixan during the first steps of the symptomless infection phase. Only pox2, oxo, gluc and pal genes in Altigo and pal, chs and lox genes in Premio were up-regulated at some time points. Basal cultivar-dependent resistance against Z. tritici could therefore be explained by various gene expression patterns rather than high expression levels of given genes. During the necrotrophic phase, Z. tritici cell wall-degrading enzyme activity levels were lower in Altigo and Premio than in Alixan, and were associated more with pycnidia than with necrosis. Similar tissue colonization occurred in the three cultivars, suggesting an inhibition of the switch to the necrotrophic lifestyle in Altigo.

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