Development of Direct Real-Time PCR System Applicable to a Wide Range of Foods and Agricultural Products

Mano, Junichi; Hatano, Shuko; Futo, Satoshi; Minegishi, Yasutaka; Ninomiya, Kenji; Nakamura, Kôsuke; Kondo, Kazunari; Teshima, Reiko; Takabatake, Reona; Kitta, Kazumi

doi:10.3358/shokueishi.55.25

Cited by 9 publications

(3 citation statements)

References 20 publications

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“…In the collaborative trial, we obtained a falsenegative result in PCR analysis to detect IPC, presumably due to PCR inhibition. We therefore developed a new PCR reagent including an enzyme and buffer more tolerable to PCR inhibition materials (Mano et al 2014). By using this new PCR reagent, the false negative detection caused by PCR inhibition in group testing is expected to be reduced.…”

Section: Group Testing and Its Validationmentioning

confidence: 99%

Development and Standardization of Analytical Methods for Increasing Varieties of Genetically Modified Crops

Mano

Takabatake

Kitta

2020

JARQ

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The varieties of commercially available genetically modified (GM) crops are rapidly increasing, and this situation demands analytical methods capable of detecting recently developed GM crops. Here we review our research activities to develop and validate new analytical methods for recently distributed GM crops. For the screening analysis of GM content in analytical samples, we developed real-time PCR-based quantitative methods for two GM maize events, MIR604 and MIR162. To accurately analyze GM content irrespective of the commingling of stacked GM events, we developed the group testing method. For the comprehensive analysis of various GM events, the real-time PCR array method was established. In November 2016, the Consumer Affairs Agency of Japan released the standard testing manual including these new testing methods to ensure the validity of the food labeling system in Japan. Given the expected increase in the number of GM events to be analyzed in the future, we need to keep working toward the realization of simple and comprehensive detection and quantification methods that can be used for the increasing number of these events.

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Section: Group Testing and Its Validationmentioning

confidence: 99%

Development and Standardization of Analytical Methods for Increasing Varieties of Genetically Modified Crops

Mano

Takabatake

Kitta

2020

JARQ

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“…New technologies can also enable amplification of DNA without prior intensive extraction, as was shown for leaf and seed tissues of MON 810, using loop-mediated isothermal amplification (LAMP) (Lee et al 2009) or even qPCR (Mano et al 2014) and digital PCR (Pavšič et al 2015) giving further possibilities for even more standardized procedures of testing without influences of the extraction step.…”

Section: Future Perspectivesmentioning

confidence: 99%

Extraction of DNA from different sample types – a practical approach for GMO testing

Žel,

Demšar,

Štebih

et al. 2015

ABS

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Current methods based on DNA targets for the detection, identification and quantification of genetically modified organisms (GMOs) involve extraction of the DNA. Different extraction procedures have been developed for the great variety of samples from food, feed, seeds and particular plant parts. This makes the operation of routine analytical laboratories complex and workloads heavy. Here we present a decision-making system, developed over many years of GMO testing on different samples, that result in the application of only a few extraction methods for the majority of samples. Developed decision-making system enables quicker and more cost effective testing of GMOs. In addition, the performance of DNA extraction resulting from the use of the selected extraction methods is presented for use in subsequent testing of GMOs by real time PCR methods. This approach can be used as a model for similar systems based on nucleic acid analysis in food, feed, seeds and plants.

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“…There are few methods that do not require initial treatments, such as PCR or extraction and purification from biological samples using immune chromatography, often used in clinics [8]. These methods are simple and rapid and may be useful in several fields but often give false-positive or false-negative results because of the presence of unrelated DNA or inhibitors or quenchers [9]. Because false results lead to wrong treatment of patients, these methods may cause unintended health hazards or critical illnesses [10].…”

Section: Introductionmentioning

confidence: 99%

DNA Detection Technology Using Zinc Finger Protein

Abe¹,

Ikebukuro²

2015

J Microb Biochem Technol

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With recent advances in nanotechnology and sequencing technology, DNA diagnostic technology is becoming practical and is advancing at a fast pace. In these detection systems, mainly PCR (particularly real-time PCR) and DNA probe hybridization techniques are used. We suggest that detecting PCR products using double-stranded DNA (dsDNA) is more convenient and powerful compared with DNA probe hybridization technique. Zinc finger protein is the major DNA binding protein in nature and it recognizes dsDNA with sequence specific manner. Additionally, by changing its amino acids sequence, we can design it to recognize the desired DNA sequence to some extent. Using zinc finger protein for DNA detection element, simple, accurate and sensitive DNA detection can be achieved. In this review, dsDNA detection using zinc finger protein is described and compared with recent advanced technology.

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Development of Direct Real-Time PCR System Applicable to a Wide Range of Foods and Agricultural Products

Cited by 9 publications

References 20 publications

Development and Standardization of Analytical Methods for Increasing Varieties of Genetically Modified Crops

Development and Standardization of Analytical Methods for Increasing Varieties of Genetically Modified Crops

Extraction of DNA from different sample types – a practical approach for GMO testing

DNA Detection Technology Using Zinc Finger Protein

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