Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay––assay validation for human milk

Sugawara, Yukio; Saito, Koichi; Ogawa, Masahiko; Kobayashi, Susumu; Shan, Guomin; Sanborn, James R.; Hammock, Bruce D.; Nakazawa, Hiroyuki; Matsuki, Yasuhiko

doi:10.1016/s0045-6535(01)00267-3

Cited by 24 publications

(18 citation statements)

References 7 publications

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“…Previously, this ELISA was demonstrated as being a potential screening method for dioxins in fish, eggs, and milk when cleanup methods are used (Shan et al, 2001;Sugawara et al, 2002). Due to the complicated sediment and soil sample matrices in this study, cleanup steps were also necessary.…”

Section: Elisa Methods Performancementioning

confidence: 99%

“…As the TCDD results were found to be approximately 130% of the TMDD response in the ELISA, TMDD measurements were multiplied by a conversion factor of 1.3 to obtain TCDD equivalent measurements (Sugawara et al, 2002;Nichkova et al, 2004). Most of the PCDDs and PCDFs with a high TEF value (>0.1) showed high to moderate cross-reactivity in the ELISA (Shan et al, 2001).…”

Section: Elisa Methods Performancementioning

confidence: 99%

“…The development of immunoassays for the PCDDs is complicated by their lipophilic properties, required method sensitivity, and the complex nature of environmental samples. Due to these difficulties, only a few studies have been reported in the development of antibodies and ELISA methods for PCDDs with mixed results (Stanker et al, 1987;Sanborn et al, 1998;Sugawara et al, 1998Sugawara et al, , 2002Harrison and Carlson, 1999;Shan et al, 2001;SW846 Method 4025, 2002;Okuyama et al, 2004;Lo et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Previously, a sensitive polyclonal antibody-based ELISA method was reported Sugawara et al, 1998;Shan et al, 2001). Different solvent systems including combinations of methanol and dimethyl sulfoxide (DMSO) to enhance analyte solubility, in combination with phosphate buffer for antibody performance, were evaluated and applied successfully to milk, soil, and biota samples (Shan et al, 2001;Sugawara et al, 2002;Nichkova et al, 2004;Nording et al, 2006). In these previous studies, cleanup procedures were required and comparable results were obtained between the ELISA and GC/HRMS methods for a small sample set (n < 20).…”

Section: Introductionmentioning

confidence: 99%

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An enzyme-linked immunosorbent assay for the determination of dioxins in contaminated sediment and soil samples

Emon¹,

Chuang²,

Lordo³

et al. 2008

Chemosphere

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A 96-microwell enzyme-linked immunosorbent assay (ELISA) method was evaluated to determine PCDDs/PCDFs in sediment and soil samples from an EPA Superfund site. Samples were prepared and analyzed by both the ELISA and a gas chromatography/high resolution mass spectrometry (GC/HRMS) method. Comparable method precision, accuracy, and detection level (8 ng kg −1 ) were achieved by the ELISA method with respect to GC/HRMS. However, the extraction and cleanup method developed for the ELISA requires refinement for the soil type that yielded a waxy residue after sample processing. Four types of statistical analyses (Pearson correlation coefficient, paired t-test, nonparametric tests, and McNemar's test of association) were performed to determine whether the two methods produced statistically different results. The logtransformed ELISA-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin values and logtransformed GC/ HRMS-derived TEQ values were significantly correlated (r = 0.79) at the 0.05 level. The median difference in values between ELISA and GC/HRMS was not significant at the 0.05 level. Low false negative and false positive rates (<10%) were observed for the ELISA when compared to the GC/HRMS at 1000 ng TEQ kg −1 . The findings suggest that immunochemical technology could be a complementary monitoring tool for determining concentrations at the 1000 ng TEQ kg −1 action level for contaminated sediment and soil. The ELISA could also be used in an analytical triage approach to screen and rank samples prior to instrumental analysis.

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Section: Elisa Methods Performancementioning

confidence: 99%

Section: Elisa Methods Performancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An enzyme-linked immunosorbent assay for the determination of dioxins in contaminated sediment and soil samples

Emon¹,

Chuang²,

Lordo³

et al. 2008

Chemosphere

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show abstract

“…For PCB levels in fish extracts, Zajicek et al found an r 2 -value of 0.75 [28]. A good correlation (r 2 = 0.92) between chemical (GC/MS) determinations and immunoassay results was also reported in a study on dioxin compounds in human milk [30].…”

Section: Immunoassaysmentioning

confidence: 82%

The potential of mechanism-based bioanalytical tools in ecotoxicological exposure and effect assessment

Eggen

Segner

2003

Analytical and Bioanalytical Chemistry

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The current challenge to ecotoxicology is to develop tools that allow rapid and cost-efficient detection of those environmental chemicals or their combinations that are responsible for sublethal, chronic toxic effects in exposed organisms. Bioanalytical tools may meet these challenges, particularly if they are mechanism-based. Technically, bioanalytical tools allow rapid and cost-efficient analysis of environmental matrices. Mechanism-based, bioanalytical tools, however, do not only indicate that certain chemicals are there, but-and this is the major advantage of mechanism-based bioanalytical tools (MBBTs)-they indicate that chemicals with a specific mode of toxic action or a specific toxic potential are there. In this way MBBTs bridge exposure and effect assessment and help in a faster identification of the causative agent(s). Several principles of MBBTs, including immunoassays, enzyme inhibition assays, receptor assays and gene induction assays are briefly discussed and their application in processes such as bioassay-directed fractionation is illustrated. The focus of this manuscript is the analytical power of MBBTs in exposure and effect assessment. MBBTs have, however, a much broader potential and can support research on other challenges in ecotoxicology such as mixture effects or multiple effects caused by single pollutants or by various stresses simultaneously.

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Methods and Applications of Toxicity Assays for Chemicals

Peng

2009

Pharmaceutical Bioassays

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Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay––assay validation for human milk

Cited by 24 publications

References 7 publications

An enzyme-linked immunosorbent assay for the determination of dioxins in contaminated sediment and soil samples

An enzyme-linked immunosorbent assay for the determination of dioxins in contaminated sediment and soil samples

The potential of mechanism-based bioanalytical tools in ecotoxicological exposure and effect assessment

Methods and Applications of Toxicity Assays for Chemicals

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