Development of Dioctadecyldimethylammonium Bromide/Monoolein Liposomes for Gene Delivery

Silva, J. P. Neves; Oliveira, A. C.; Gomes, Andreia C.; Oliveira, M. Elisabete

doi:10.5772/48603

Cited by 5 publications

(5 citation statements)

References 131 publications

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“…The plateau value is reached at CR (+/−) = 2.0 for DNA/DODAB:MO (2:1) lipoplexes, CR (+/−) = 1.5 for DNA/DODAB:MO (1:1) lipoplexes and CR (+/−) = 1.0 for DNA/ DODAB:MO (1:2) lipoplexes, which shows the fluidizing effect of MO within the lipoplex structure (already observed by Nile Red anisotropy measurements, Fig. 5), in agreement to the previously reported studies [19,23]. The final FRET efficiency (ϕ FRET ) obtained for all DNA/DODAB: MO lipoplexes at CR (+/−) = 4.0 shows a clear distinction between MO-rich formulations (1:2), with a ϕ FRET of 90%, and DODAB-rich formulations (2:1), with a ϕ FRET of 70%.…”

Section: Effect Of Ph and Anionic/neutral Lipids On Dna Dissociation ...supporting

confidence: 92%

“…e l s e v i e r . c o m / l o c a t e / b b a m e m [23]. Nevertheless, the system has only been studied until now for exceeding molar fractions of DODAB, where a multilamellar organization is predominant [23].…”

Section: Introductionmentioning

confidence: 99%

“…c o m / l o c a t e / b b a m e m [23]. Nevertheless, the system has only been studied until now for exceeding molar fractions of DODAB, where a multilamellar organization is predominant [23]. In this work, we studied the behavior of pDNA/DODAB:MO lipoplexes at high MO contents (1:2 and 1:4 DODAB:MO ratios), where inverted bicontinuous non-lamellar phases are more likely to form and remain stabilized [24].…”

Section: Introductionmentioning

confidence: 99%

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Structural dynamics and physicochemical properties of pDNA/DODAB:MO lipoplexes: Effect of pH and anionic lipids in inverted non-lamellar phases versus lamellar phases

Silva

Oliveira

et al. 2014

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Dioctadecyldimethylammonium bromide (DODAB):Monoolein (MO) lipoplexes have mainly been studied within the range of high molar ratios of DODAB, with noticeable transfection efficiencies in the Human Embryonic Kidney (HEK, a.k.a. 293T) cell line. In this work, we intend to study the effect of high MO content on the structure and physicochemical properties of pDNA/DODAB:MO lipoplexes to achieve some correlation with their transfection efficiency. Static/Dynamic Light Scattering and Cryo-TEM imaging were used to characterize the size/morphology of DNA/DODAB:MO lipoplexes at different DODAB:MO contents (2:1, 1:1, 1:2) and charge ratios (CRs) (+/-). Nile Red fluorescence emission was performed to detect changes in microviscosity, hydration and polarity of DNA/DODAB:MO systems. Lipoplexes stability at physiological pH values and in the presence of anionic lipids was evaluated by Förster Resonance Energy Transfer (FRET). Physicochemical/structural data were complemented with transfection studies in HEK cells using the β-galactosidase reporter gene activity assay. This work reports the coexistence of multilamellar and non-lamellar inverted phases in MO-richer lipoplexes (DODAB:MO 1:2 and 1:4), leading to transfection efficiencies comparable to those of multilamellar (DODAB-richer) lipoplexes, but at higher charge ratios [CR (+/-)=6.0] and without dose-effect response. These results may be related to the structural changes of lipoplexes promoted by high MO content.

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Section: Effect Of Ph and Anionic/neutral Lipids On Dna Dissociation ...supporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Structural dynamics and physicochemical properties of pDNA/DODAB:MO lipoplexes: Effect of pH and anionic lipids in inverted non-lamellar phases versus lamellar phases

Silva

Oliveira

et al. 2014

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…200 The Benedictines also "stayed at the home of D. Luiza Grimalda [sic], Capitoa and governadora," while construction was underway. 201 The 193 "Enformação e cópia de Certidões que pertenecem a residencia dos nossos nas aldeas dos Franciscans similarly benefited from Luisa's direction, being granted the site of the Morro do Moreno "from the foot of the said mountain to the top," if, as the document suggested, the Franciscans wanted to collect stone from their new quarry to finish expanding the existing hermitage at the peak. 202 Although the original document is lost, a copy survives at the National Library in Rio de Janeiro.…”

Section: Luisa Grimaldi and Colonial Negotiation In Espírito Santo (1...mentioning

confidence: 99%

Governadoras: Women Administrators, Gender, and Colonization in Sixteenth-Century Portuguese America

O'Leary

2024

Renaiss. Q.

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In sixteenth-century Brazil, several European women governed the captaincies of their late or absent husbands during the first century of Portuguese colonization. A contextual and lexical analysis of the male-authored sources reveals that these women acted decisively to protect and expand familial patrimonies and, in doing so, were part of the colonizing movement. Although extensive written evidence survives that attests to their authority and agency over colonial affairs, their importance has been overlooked in the scholarship. Therefore, this essay argues that a small group of elite European women became imperial agents who wielded power against colonial subjects in select circumstances.

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“…The low pH combined with the presence of a negatively charged ECM provides a good targeting lever to develop electrostatic charge-based targeting of LNPs to target cells. Cationic liposomes have been envisioned as a desirable delivery system that can target GAGs [111,112]. Charged-based removal of the PEG coating for tumor targeting largely uses two key approaches: (i) PEG removal by employing a pH-sensitive linker in a PEG-lipid and (ii) concentration of PEG in domains on the LNP surface, thereby leaving a large part of LNPs exposed to target cell surfaces.…”

Section: Target Cell Interanalization-solid Tumorsmentioning

confidence: 99%

Key Design Features of Lipid Nanoparticles and Electrostatic Charge-Based Lipid Nanoparticle Targeting

Gyanani

Goswami

2023

Pharmaceutics

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Lipid nanoparticles (LNP) have gained much attention after the approval of mRNA COVID-19 vaccines. The considerable number of currently ongoing clinical studies are testament to this fact. These efforts towards the development of LNPs warrant an insight into the fundamental developmental aspects of such systems. In this review, we discuss the key design aspects that confer efficacy to a LNP delivery system, i.e., potency, biodegradability, and immunogenicity. We also cover the underlying considerations regarding the route of administration and targeting of LNPs to hepatic and non-hepatic targets. Furthermore, since LNP efficacy is also a function of drug/nucleic acid release within endosomes, we take a holistic view of charged-based targeting approaches of LNPs not only in the context of endosomal escape but also in relation to other comparable target cell internalization strategies. Electrostatic charge-based interactions have been used in the past as a potential strategy to enhance the drug release from pH-sensitive liposomes. In this review, we cover such strategies around endosomal escape and cell internalization in low pH tumor micro-environments.

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Development of Dioctadecyldimethylammonium Bromide/Monoolein Liposomes for Gene Delivery

Cited by 5 publications

References 131 publications

Structural dynamics and physicochemical properties of pDNA/DODAB:MO lipoplexes: Effect of pH and anionic lipids in inverted non-lamellar phases versus lamellar phases

Structural dynamics and physicochemical properties of pDNA/DODAB:MO lipoplexes: Effect of pH and anionic lipids in inverted non-lamellar phases versus lamellar phases

Governadoras: Women Administrators, Gender, and Colonization in Sixteenth-Century Portuguese America

Key Design Features of Lipid Nanoparticles and Electrostatic Charge-Based Lipid Nanoparticle Targeting

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