Development of digital light processing-based multi-material bioprinting for fabrication of heterogeneous tissue constructs

Su, Hao; Lu, Bingxian; Li, Mengran; Yang, Xue; Qin, Minghao; Yang, Wu

doi:10.1039/d3bm01054f

Cited by 5 publications

(2 citation statements)

References 65 publications

(83 reference statements)

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“…For example, since cells are often present in the form of a suspension in hydrogels, their absorption, reflection, and refraction to the curing light might affect the quality of bioprinting, which requires careful selection of the wavelength of the curing light and the cell density. 22 In addition, the choice of materials that are suitable for vat photopolymerization is limited, resulting in only a few acrylate materials being available so far. Breakthroughs in photochemistry are expected to expand the range of photopolymerizable biomaterials.…”

Section: Bioprinting Techniquesmentioning

confidence: 99%

Organ bioprinting: progress, challenges and outlook

Wu,

Qin,

Yang

2023

J. Mater. Chem. B

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Section: Bioprinting Techniquesmentioning

confidence: 99%

Organ bioprinting: progress, challenges and outlook

Wu,

Qin,

Yang

2023

J. Mater. Chem. B

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“…Digital light projection 3D printing technology is a widely used type of laser‐assisted bioprinting. [ 31 , 32 ] However, a hydrogel material that simultaneously exhibits adhesion, 3D printability, and biocompatibility has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Clay Sculpture‐Inspired 3D Printed Microcage Module Using Bioadhesion Assembly for Specific‐Shaped Tissue Vascularization and Regeneration

Fang,

Ju,

Chen

et al. 2024

Advanced Science

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Abstract3D bioprinting techniques have enabled the fabrication of irregular large‐sized tissue engineering scaffolds. However, complicated customized designs increase the medical burden. Meanwhile, the integrated printing process hinders the cellular uniform distribution and local angiogenesis. A novel approach is introduced to the construction of sizable tissue engineering grafts by employing hydrogel 3D printing for modular bioadhesion assembly, and a poly (ethylene glycol) diacrylate (PEGDA)‐gelatin‐dopamine (PGD) hydrogel, photosensitive and adhesive, enabling fine microcage module fabrication via DLP 3D printing is developed. The PGD hydrogel printed micocages are flexible, allowing various shapes and cell/tissue fillings for repairing diverse irregular tissue defects. In vivo experiments demonstrate robust vascularization and superior graft survival in nude mice. This assembly strategy based on scalable 3D printed hydrogel microcage module could simplify the construction of tissue with large volume and complex components, offering promise for diverse large tissue defect repairs.

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Multi‐Material Digital Light Processing (DLP) Bioprinting of Heterogeneous Hydrogel Constructs with Perfusable Networks

Yang,

Chu,

Zhuang

et al. 2024

Adv Funct Materials

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The challenges of replicating the complex mechanical and structural diversity of natural tissues in vitro by leveraging multi‐material digital light processing (DLP) bioprinting are addressed. This technique utilizes PEGDA‐AAm bio‐ink to develop multi‐component, cell‐laden hydrogel constructs with varying moduli. These constructs not only possess heterogeneous mechanical properties but also feature complex architectures and precisely engineered surface microstructures. The hydrogel microfluidic chips are successfully fabricated with perfusable microchannels, embedding various cell types within the matrix. This approach enables the bioprinting of intricate cell‐laden structures with unique surface topologies, such as spiral grooves and triply periodic minimal surfaces (TPMS), which effectively influence cell alignment, spreading, and migration. By integrating various cell‐laden PA hydrogels, the diverse mechanical moduli of biological tissues, including bone, liver lobules, and vascular networks are replicated. This technique ensures high‐fidelity differentiation between cell types and regions. These findings provide valuable insights into the impact of substrate modulus and structure on cell behavior, underscoring the potential of multi‐component, multi‐modulus hydrogel constructs in creating sophisticated structures with custom‐tailored mechanical properties. This study significantly advances the field by demonstrating the feasibility and effectiveness of multi‐material DLP bioprinting in developing complex, functionally relevant tissue models for tissue engineering and regenerative medicine.

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Development of digital light processing-based multi-material bioprinting for fabrication of heterogeneous tissue constructs

Abstract: Human tissues and organs have heterogeneous structures with multiple property gradients, which are difficult to be restored by single-material bioprinting technology. The advances in multi-material bioprinting technologies have shown great...

Cited by 5 publications

References 65 publications

Organ bioprinting: progress, challenges and outlook

Organ bioprinting: progress, challenges and outlook

Clay Sculpture‐Inspired 3D Printed Microcage Module Using Bioadhesion Assembly for Specific‐Shaped Tissue Vascularization and Regeneration

Multi‐Material Digital Light Processing (DLP) Bioprinting of Heterogeneous Hydrogel Constructs with Perfusable Networks

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