Development of digestive enzymes in larvae of Solea senegalensis, Kaup 1858

Ribeiro, Laura; Zambonino-Infante, José‐Luis; Cahu, Chantal; Dinis, María Teresa

doi:10.1016/s0044-8486(99)00180-5

Cited by 226 publications

(198 citation statements)

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“…trypsin, pepsin) could be accepted as comparative deterministic tool as well as a predictor not only for description of nutritional capabilities but also quality indicators of marine fish larvae during early ontogeny (Ribeiro et al, 1999;Lazo et al, 2000;Cara et al, 2003;Suzer et al, 2006bSuzer et al, , 2007aSuzer et al, ,b, 2013Ribeiro et al, 2008;Gisbert et al, 2009). Besides, knowledge about larval nutritional status and also ontogenetical changes of digestive enzymes in larvae of new candidate species will be supplied the development of processes of food digestion and assimilation.…”

Section: Discussionmentioning

confidence: 99%

“…During the last two decades, numerous studies have shown that early ontogeny of the digestive enzymes could be evaluated as effective tool for determination of larval quality, performance and nutritional status and physiological response for preferred diet during both larval and weaning stage under culture conditions (Ribeiro et al, 1999;Lazo et al, 2000;Cara et al, 2003;Suzer et al, 2006b;Suzer et al, 2007a,b;Ribeiro et al, 2008;Gisbert et al, 2009;Suzer et al, 2013). Moreover, there are some studies related with D. dentex focused on larval development (Rueda and Martínez, 2001), histological ontogeny of the digestive tract (Santamaría et al, 2004), early life history (Fırat et al, 2005), and different culture techniques (Suzer et al, 2006a) of D. dentex however, some detailed investigations about digestive enzymes were conducted by SDS-PAGE (Alarcón et al, 1998) and spectrophotometric ) methods and also effects of different dietary formulations (Pérez-Jiménez et al, 2009) in this species under culture conditions.…”

Section: Introductionmentioning

confidence: 99%

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Suzer

Çoban²,

Yıldırım³

et al. 2014

Turk. J. Fish. Aquat. Sci.

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Stage-specific ontogeny of main digestive enzymes in common dentex, Dentex dentex, were assayed during early life development according to notochord flexion studied from hatching to 45 days after hatching (DAH). The notochord was straight in yolk-sac larvae of D. dentex and preflexion stage was observed between 0 and 18 DAH as 2.75 and 6.27 mm at total length (TL). Then, flexion of the notochord was estimated between 19 and 30 DAH as 6.27 and 10.9 mm at TL. Important alterations in specific activities of all digestive enzymes measured during period of this study were mostly related with metamorphosis and co-feeding. Trypsin and chymotrypsin specific activities were firstly detected on day 3 and were related with mouth opening, whereas they slightly increased until 25 DAH respectively. Pepsin was firstly detected on day 28 and related with stomach formation and a sharp increase was observed until 30 DAH. Both amylase and lipase were measured on day 2 and 4, respectively and they also sharply increased during the first week of life. Additionally, except pepsin, most of the digestive enzyme activities were firstly secreted in the preflexion stage and also gastric secretion started and functional development digestive system were occurred in in flexion and postflexion stages, respectively.Keywords: Digestive enzymes, stage-specific ontogeny, larval development, Dentex dentex, common dentex. Kültürü Yapılan Sinarit (Dentex dentex) Larvalarında Sindirim Enzimlerinin Dönemsel Gelişimi ÖzetBu çalışmada, sinarit (Dentex dentex) larvalarında açılımdan 45. güne kadar geçen erken dönem boyunca önemli sindirim enzimlerinin dönemsel ontogenileri notokorda bükülmesine göre incelenmiştir. Besin keseli sinarit larvalarında notokorda düz yapıda gözlenmiş ve bükülme öncesi dönem 0 ile 18. günler arasında 2,75 ile 6,27 mm total boy uzunlukları arasında izlenmiştir. Ardından, notokordanın bükülmesi ise 19 ile 30. günler arasında 6,27 ile 10,9 mm total boy uzunlukları arasında tespit edilmiştir. Çalışma boyunca izlenen sindirim enzimleri aktivitelerindeki önemli değişimlerin çoğunlukla metamorfoz ve toz yeme geçiş süreci ile ilişkili olduğu bulunmuştur. Tripsin ve kimotripsin speisifk aktiviteleri larvalarda ağız açılımı ile birlikte ilk olarak 3. günde tespit edilmiş ve 25. güne kadar yavaş bir artış göstermiştir. Pepsin aktivitesi ise mide oluşumu ile eş zamanlı olarak ilk kez 28. günde gözlenmiş ve 30. güne kadar ani bir artış sergilemiştir. Amilaz ve lipaz aktivitesi ise sırasıyla 2. ve 4. günlerde izlenmiş ve sonrasında larval dönemin ilk haftası boyunca hızlı şekilde yükselmiştir. Ayrıca, pepsin dışındaki tüm sindirim enzimleri aktivitesi ilk olarak bükülme öncesi dönemde izlenmiş ve gastrik salgının başlangıcı ve fonksiyonel sindirim sisteminin oluşması sırasıyla bükülme ve bükülme sonrası dönemde tespit edilmiştir.Anahtar Kelimeler: Sindirim enzimleri, dönemsel ontogeni, larval gelişim, Dentex dentex, sinarit.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Untitled

Suzer

Çoban²,

Yıldırım³

et al. 2014

Turk. J. Fish. Aquat. Sci.

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“…ssetryp1 was the most abundant, with expression greatly activated early in the first week of larval life. This expression pattern is well-correlated with a total 4 trypsin activity peak at 9 days after hatching (DAH) (Ribeiro et al, 1999). Moreover, trypsin enzymatic activity has been proposed as a good indicator of nutritional status in young sole (Engrola et al, 2007).…”

Section: Introductionmentioning

confidence: 89%

Effect of different diets on proteolytic enzyme activity, trypsinogen gene expression and dietary carbon assimilation in Senegalese sole (Solea senegalensis) larvae

Gamboa‐Delgado

Vay

Fernández‐Díaz

et al. 2011

Comparative Biochemistry and Physiology Part B: Biochemistry an

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“…Much research has been conducted in the last two decades to study the digestive ability and specific nutritional requirements as well as ontogenesis of digestive enzymes and as developmental features of the digestive tract of fish larvae and juveniles as red drum Scienops ocellatus [3,4], gilthead sea bream Sparus aurata [5,6], European sea bass Dicentrarchus labrax [7,8], dover sole Solea solea [9], turbot Scophthalmus maximus [10][11][12], senegal sole Solea senegalensis [13], Japanese flounder Paralichthys olivaceus [14].…”

Section: Introductionmentioning

confidence: 99%

Digestive Enzymes in Larvae and Juveniles of Farmed Sharpsnout Seabream (Diplodus puntazzo) (Cetti, 1777)

Savona¹

2011

TOMBJ

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Abstract:The ontogenetic development and pattern of main digestive enzyme activities (proteases, carbohydrases and lipases) were investigated in farmed sharpsnout seabream Diplodus puntazzo larvae and juveniles, during the passage from the larval stage to adult (from 21 to 277 days after hatching -DAH). Larvae showed exponential growth, thus confirming that Rotifers and Artemia nauplii are suitable preys for early feeding of D. puntazzo larvae.Pepsin activity was low in the larvae, but it increased afterwards in relation with stomach development. In juveniles, levels of specific activity of trypsin, chymotrypsin and elastase were lower compared to that recorded in larvae. A decrease in the activity of the alkaline proteases could be related to an increase in the functionality of stomach. Qualitative and quantitative assay of glycosidases ( -amylase, cellulase and maltase) showed high glycosidase activity, indicating that D. puntazzo is capable of digesting carbohydrates in its diet. Levels of lipase activity in juveniles were higher than those found in larvae. Our results didn't show an inductive character of lipases, since in juveniles the activity levels were almost constant even if the fat content of compound diet varied (from 7.0 % to 20 %).The rapid changes in the digestive activities of D. puntazzo larvae supported the existence of well established induction and regulation digestive mechanisms. Present results also confirm that D. puntazzo larvae have complete digestive enzymatic equipment that increased during larval ontogeny and enable larvae to digest and absorb nutrients contained in live preys once exogenous feeding occurred. The enzymatic pattern is correlated with omnivorous feeding habits of the species. Moreover, the patterns of activity of the main digestive enzymes of D. puntazzo larvae and juveniles indicated the early functional development of their digestive system and suggested this species as a good candidate for Mediterranean aquaculture using the compound diet.

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Development of digestive enzymes in larvae of Solea senegalensis, Kaup 1858

Cited by 226 publications

References 23 publications

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Untitled

Effect of different diets on proteolytic enzyme activity, trypsinogen gene expression and dietary carbon assimilation in Senegalese sole (Solea senegalensis) larvae

Digestive Enzymes in Larvae and Juveniles of Farmed Sharpsnout Seabream (Diplodus puntazzo) (Cetti, 1777)

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