Development of Diagnostic PCR System for Three Seedtransmitted Quarantine Viruses Associated with Cucurbitaceae

Abstract

The Cucurbitaceae are a plant family that consist of over a hundred genera, the most important of which are squash, pumpkin, zucchini, some gourds, cucumber and watermelon. These are among the top imported seeds in Korea. At the time of their import, the Squash mosaic virus (SqMV), the Cucumber green mottle mosaic virus (CGMMV) and the Kyuri green mottle mosaic virus (KGMMV) are designated as regulated viruses for quarantine in Korea. This study was conducted to develop specific primer sets for easy and rapid detection of SqMV, CGMMV and KGMMV. RT-PCR with the nested PCR primer sets and modified positive control plasmids were capable of highly sensitive detection and verification of such viruses. In addition, developed diagnostic PCR systems applied to quarantine sites detected 47 cases of SqMV, 67 cases of CGMMV and 17 cases of KGMMV between 2010 and the first half of 2014.

Development of Nested PCR-Based Specific Markers for Detection of Peach Rosette Mosaic Virus in Plant Quarantine

“…The RT-PCR-marker sets, the nested PCR marker sets, and the modified-plasmid positive control were used to promptly and accurately diagnose PRMV, which is an unreported controlled-quarantine virus in Korea. Previously studies, we have been developed fast and accurately diagnosis system that is expected and detected to viruses [15][16][17]. The developed method was applied to quarantine sites, and it is expected that it will contribute to plant-quarantine processes in Korea.…”