Development of CRISPR/Cas9 plasmid for multiple sites genome editing in oil palm (Elaeis guineensis Jacq.)

Aprilyanto, Victor; Darmawan, Chris; Utomo, Condro; Liwang, Tony

doi:10.1063/1.5098407

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Recently, a group designed an expression cassette to target four genes. However, it is not well known whether the expression cassette and CRISPR/Cas9 vector successfully transformed into oil palm ( Aprilyanto et al., 2019 ). Above mentioned studies report that CRISPR/Cas9 successfully use in oil palm genome editing ( Yeap et al., 2021 ).…”

Section: Recent Developments In Genome Editing In Palm Treesmentioning

confidence: 99%

“…The CRISPR/Cas9 vector construct is one of the crucial elements for its effective performance. Additionally, when more genes responsible for oil palm agro-economical features are discovered, editing at a single locus in the genome may not produce the desired result if numerous genes rule the trait ( Aprilyanto et al., 2019 ). Moreover, the critical step in genome editing is the selection of sgRNA (target region in the genome).…”

Section: Limitations In Palm Trees Crispr-based Genome Editingmentioning

confidence: 99%

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Is CRISPR/Cas9 a way forward to fast-track genetic improvement in commercial palms? Prospects and limits

et al. 2022

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Commercially important palms (oil palm, coconut, and date palm) are widely grown perennial trees with tremendous commercial significance due to food, edible oil, and industrial applications. The mounting pressure on the human population further reinforces palms’ importance, as they are essential crops to meet vegetable oil needs around the globe. Various conventional breeding methods are used for the genetic improvement of palms. However, adopting new technologies is crucial to accelerate breeding and satisfy the expanding population’s demands. CRISPR/Cas9 is an efficient genome editing tool that can incorporate desired traits into the existing DNA of the plant without losing common traits. Recent progress in genome editing in oil palm, coconut and date palm are preliminarily introduced to potential readers. Furthermore, detailed information on available CRISPR-based genome editing and genetic transformation methods are summarized for researchers. We shed light on the possibilities of genome editing in palm crops, especially on the modification of fatty acid biosynthesis in oil palm. Moreover, the limitations in genome editing, including inadequate target gene screening due to genome complexities and low efficiency of genetic transformation, are also highlighted. The prospects of CRISPR/Cas9-based gene editing in commercial palms to improve sustainable production are also addressed in this review paper.

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Section: Recent Developments In Genome Editing In Palm Treesmentioning

confidence: 99%

Section: Limitations In Palm Trees Crispr-based Genome Editingmentioning

confidence: 99%

Is CRISPR/Cas9 a way forward to fast-track genetic improvement in commercial palms? Prospects and limits

et al. 2022

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“…GE technology has been applied to engineer fatty acid biosynthetic pathways in soybean, [4,5,6], canola [7], Camelina sativa [8], peanut [9], and oil palm [10]. Based on the successful stories on those plants it shown that this technique can be applied to oil palm.…”

Section: Introductionmentioning

confidence: 99%

Transformation of CRISPR/Cas9 expression construct to enhance saturated fatty acid synthesis in oil palm for biofuel production

Minarsih,

Permatasari,

Setiawati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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New technology innovations and the utilization of elite varieties are necessary for oil palm intensification to improve productivity. In addition to productivity improvement, oil palm intensification is also directed to improve the quality of crude palm oil (CPO) to be used in biofuel production. The modification of the fatty acid biosynthesis pathway through genetic engineering is one of the most promising approaches. The research aims to apply a genetic transformation to oil palm calli and to improve success regeneration of the transformed explants. A CRISPR/Cas9 expression construct to edit the PTE (palmitoyl-acyl carrier protein thioesterase) gene, which protein is involved in the formation of palmitic acid, was generated in the previous research. In this study, the genetic transformation of the construct into 4month-old oil palm calli was conducted via Agrobacterium tumefaciens. The transformed calli were subsequently subcultured into selection media containing 100 ppm of cefotaxime and 50 ppm of kanamycin antibiotics. After several weeks, the transformed calli were transferred into DF10 media without antibiotics. Non-transformed calli were able to grow on the selection media with antibiotics but still in the globular phase. In contrast, the transformed calli on the selection media were able to grow to the scutellar phase with a compact structure in the embryogenic callus development stages. The next step is to optimize the transformation method as well as the regeneration media composition.

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Date Palm Genetic Identification and Improvement Utilizing Molecular Markers and DNA Barcoding

Mahdy

El-Sharabasy

2021

The Date Palm Genome, Vol. 1

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Development of CRISPR/Cas9 plasmid for multiple sites genome editing in oil palm (Elaeis guineensis Jacq.)

Cited by 4 publications

References 15 publications

Is CRISPR/Cas9 a way forward to fast-track genetic improvement in commercial palms? Prospects and limits

Is CRISPR/Cas9 a way forward to fast-track genetic improvement in commercial palms? Prospects and limits

Transformation of CRISPR/Cas9 expression construct to enhance saturated fatty acid synthesis in oil palm for biofuel production

Date Palm Genetic Identification and Improvement Utilizing Molecular Markers and DNA Barcoding

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