Development of Cowpea Mosaic Virus as a High-Yielding System for the Presentation of Foreign Peptides

Porta, Claudine; Spall, V. E.; Loveland, Jane; Johnson, John E.; Barker, Paul D.; Lomonossoff, George P.

doi:10.1006/viro.1994.1417

Cited by 202 publications

(125 citation statements)

References 0 publications

Supporting

Mentioning

124

Contrasting

Order By: Relevance

“…The principle that chimeric virus particles had potential as protective immunogens was thus established with TMV, as well as with the more commonly known hepatitis B surface antigen particles (Valenzuela et al 1985). After it became possible to synthesize infectious RNA from cloned cDNA copies of plant viral genomes, chimeric virus particles derived from TMV and other viruses were shown to present foreign peptides on their surface without signi¢cantly inhibiting the ability of the viruses to replicate and to infect whole plants systemically (Porta et al 1994;Turpen et al 1995;Fitchen et al 1995).…”

Section: A Historical Overviewmentioning

confidence: 99%

Tobacco mosaic virus and the virescence of biotechnology

Turpen¹

1999

Phil. Trans. R. Soc. Lond. B

View full text Add to dashboard Cite

There is a growing realization that a modern combination of molecular biology and agriculture will provide a photosynthetic basis for the biosynthesis of an increasing variety of complex and valuable molecules. This`greening' of biotechnology may impact on the global environment in many bene¢cial ways, but will perhaps have its most signi¢cant impact on human health. In the past decade, the capacity to use plants as an expanded source of therapeutics has grown through the accelerated development of e¡ective viral transfection vectors for gene transfer to cultivated crops. Recombinant vectors based on tobacco mosaic virus (TMV) and other members of the Tobamovirus genus are now used to transfect commercially meaningful quantities of plant biomass cultivated in enclosed greenhouses and multiacre ¢elds. Viral RNA promoters are e¡ectively manipulated for the synthesis of recombinant messenger RNAs in whole plants. Chimeric plant virus and virus-like particles are designed for peptide production and display from recombinant structural protein^gene fusions. Gene functions are assessed and modi¢ed by either virus-mediated expression or cytosolic inhibition of expression at the RNA level. Recombinant virus populations, propagated by inoculating plants with infectious RNA transcripts or recombinant virions, have proved to be genetically stable over product-manufacturing cycles. Large volumes of highly puri¢ed protein products isolated from transfected foliage conform reproducibly to the speci¢cations required for well-characterized biologics. In some cases, they exceed the speci¢c activities of molecules puri¢ed from alternative recombinant and native sources. The resulting products are then formulated according to the developing national regulatory guidelines appropriate for agriculture-based manufacturing. Each of these innovations was ¢rst realized by researchers using clones of tobamovirus genes and recombinant genomes. This progress is founded on the heritage of a century of fundamental TMV research.

show abstract

Section: A Historical Overviewmentioning

confidence: 99%

Tobacco mosaic virus and the virescence of biotechnology

Turpen¹

1999

Phil. Trans. R. Soc. Lond. B

View full text Add to dashboard Cite

show abstract

“…In an effort to produce effective non-replicating vaccines, we have been examining the vaccine potential of a plant virus, cowpea mosaic virus (CPMV), genetically engineered (Usha et al, 1993 ;Porta et al, 1994) to express and display foreign peptides on its surface. In this presentation system, termed EPICOAT, peptides of up ~zl., 199.5, 1996;Buratti et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

A chimaeric plant virus vaccine protects mice against a bacterial infection

Brennan¹,

Gilleland

Staczek

et al. 1999

Microbiology

View full text Add to dashboard Cite

The plant virus cowpea mosaic virus (CPMV) is an efficient carrier of foreign peptides for the generation of strong humoral immune responses. Peptides derived from both viruses and bacteria are strongly immunogenic when displayed on the surface of CPMV and elicit high titres of peptide-specific antibody. However, the protective effects of antibodies generated using bacterial epitopes in this system have yet t o be demonstrated. In this study the ability of chimaeric virus particles (CVPs) t o afford protection against bacterial infection was assessed. Immunization of outbred mice with CPMV expressing a peptide derived from outer-membrane protein F of Pseudomonas aeruginosa (CPMV-PAE5) generated high titres of P. aeruginosa-specif ic IgG that opsonized the bacteria for phagocytosis by human neutrophils and afforded protection upon challenge with t w o different immunotypes of P. aeruginosa in a model of chronic pulmonary infection. When examined 8 d after challenge, CVP-immunized mice had fewer severe lung lesions and fewer bacteria in their lungs compared t o mice immunized with wild-type virus. Different levels of protection were seen with CPMV-PAE5 when Freund's or alum adjuvants were used. These studies highlight the ability of CVPs t o generate protective immunity against infectious disease agents.

show abstract

“…Different species of viruses belonging to several families and genera (1)(2)(3)(4)(5)(6)(7)(8)(9) have been used for this purpose.…”

Section: Introductionmentioning

confidence: 99%

Immune response induced in mice oral immunization with cowpea severe mosaic virus

Florindo

Aragão

Silva

et al. 2002

Braz J Med Biol Res

View full text Add to dashboard Cite

There is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. Cowpea severe mosaic virus (CPSMV), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (Vigna unguiculata) leaves infected with CPSMV both administered by gavage to Swiss mice induced a humoral immune response. Groups of 10 Swiss mice (2-month-old females) were immunized orally with 10 daily doses of either 50 µg viral capsid protein (boosters of 50 µg at days 21 and 35 after immunization) or 0.6 mg protein of the crude extract (boosters of 0.6 mg at days 21 and 35 after immunization). Anti-CPSMV antibodies were quantified by ELISA in pooled sera diluted at least 1:400 at days 7, 14, 21, 28, 35 and 42 after the 10th dose. IgG and IgA against CPSMV were produced systemically, but IgE was not detected. No synthesis of specific antibodies against the proteins of leaf extracts from V. unguiculata, infected or not with CPSMV, was detected. The use of CPSMV, a plant-infecting virus that apparently does not induce a pathogenic response in animals, induced a humoral and persistent (at least 6 months) immune response through the administration of low antigen doses by gavage. These results raise the possibility of using CPSMV either as a vector for the production of vaccines against animal pathogens or in quick and easy methods to produce specific antisera for viral diagnosis.

show abstract

Development of Cowpea Mosaic Virus as a High-Yielding System for the Presentation of Foreign Peptides

Cited by 202 publications

References 0 publications

Tobacco mosaic virus and the virescence of biotechnology

Tobacco mosaic virus and the virescence of biotechnology

A chimaeric plant virus vaccine protects mice against a bacterial infection

Immune response induced in mice oral immunization with cowpea severe mosaic virus

Contact Info

Product

Resources

About