Development of conventional subunit vaccines for anaplasmosis and babesiosis

Montenegro-James, S.; Johnson, Wendell C.; Goff, Will L.

doi:10.1016/0304-4017(94)03124-f

Veterinary Parasitology

1995

DOI: 10.1016/0304-4017(94)03124-f

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Development of conventional subunit vaccines for anaplasmosis and babesiosis

S. Montenegro-James¹,

Wendell C. Johnson²,

Will L. Goff³

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Cited by 6 publications

(1 citation statement)

References 41 publications

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“…These MSPs have been expressed to high levels and purified from recombinant Escherichia coli. MSP1, -2, and -4 have potential for the devel-opment of vaccines, and MSP3 and -5 have potential for use in improved diagnostic assays (3,12,20,27).…”

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confidence: 99%

Evaluation of an Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5 for Serological Diagnosis of Bovine Anaplasmosis in Venezuela

Reyna-Bello

Cloeckaert

Vizcaı́no

et al. 1998

Clin Diagn Lab Immunol

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An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of bovine anaplasmosis with purified recombinant major surface protein 5 (MSP5) of Anaplasma marginale produced in Escherichia coli. Serum antibody responses against MSP5 were detected in calves experimentally infected with A. marginale as early as 21 days postinfection and reached maximum titers at 28 days postinfection. The MSP5 ELISA performed with serum samples taken from field cattle from different regions of Venezuela showed a seroprevalence of 47%, which seems to be in accordance with the reported epidemiological status of bovine anaplasmosis in Venezuela. Positive results obtained in the MSP5 ELISA were further confirmed by immunoblotting, with the recombinant MSP5 as the antigen. Thus, these results confirmed the importance of MSP5 as a suitable antigen for the serological diagnosis of bovine anaplasmosis.

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mentioning

confidence: 99%

Evaluation of an Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5 for Serological Diagnosis of Bovine Anaplasmosis in Venezuela

Reyna-Bello

Cloeckaert

Vizcaı́no

et al. 1998

Clin Diagn Lab Immunol

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Vaccines against babesiosis using soluble parasite antigens

Schetters¹,

Montenegro-James²

1995

Parasitology Today

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Low levels of genetic diversity associated with evidence of negative selection on the Babesia bovis apical membrane antigen 1 from parasite populations in Thailand

Rittipornlertrak

Nambooppha

Simking

et al. 2017

Infection, Genetics and Evolution

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Development of conventional subunit vaccines for anaplasmosis and babesiosis

Cited by 6 publications

References 41 publications

Evaluation of an Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5 for Serological Diagnosis of Bovine Anaplasmosis in Venezuela

Evaluation of an Enzyme-Linked Immunosorbent Assay Using Recombinant Major Surface Protein 5 for Serological Diagnosis of Bovine Anaplasmosis in Venezuela

Vaccines against babesiosis using soluble parasite antigens

Low levels of genetic diversity associated with evidence of negative selection on the Babesia bovis apical membrane antigen 1 from parasite populations in Thailand

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