Development of complementary techniques applied to<i>Hydrangea</i>hybridizations

Nesi, B.; Lazzereschi, S.; Pecchioli, S.; Pacifici, S.; Venturieri, A.G.; Burchi, G.

doi:10.17660/actahortic.2015.1104.51

Cited by 2 publications

(3 citation statements)

References 9 publications

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“…Once a phenotyping system is in place, conventional and genome-enabled breeding strategies can be used to improve PM resistance in bigleaf hydrangea. Conventional breeding strategies most commonly used in bigleaf hydrangea breeding include recurrent selection, backcrossing, and wide hybridization [5,11,[89][90][91][92][93][94][95]. Recurrent selection uses controlled crosses between a susceptible and resistant parent to produce a large F1 population that is evaluated for PM resistance.…”

Section: Breeding For Powdery Mildew Resistancementioning

confidence: 99%

“…Marker-assisted and genome-enabled breeding can reduce the time to new variety development and the number of progenies to evaluate in woody ornamental breeding programs [93,95,97,98]. Tools for marker-assisted selection and genome-enabled breeding strategies in bigleaf hydrangea include a full-length, annotated genome for cultivars Endless Summer and Veitchii [99], a high-density genetic linkage map [97], and genetic markers for inflorescence shape [98,100] and double flowering [99,101].…”

Section: Breeding For Powdery Mildew Resistancementioning

confidence: 99%

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Powdery Mildew of Bigleaf Hydrangea: Biology, Control, and Breeding Strategies for Resistance

Jennings,

Baysal-Gurel,

Alexander

2024

Horticulturae

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Hydrangea macrophylla, commonly known as bigleaf, garden, French, or florist hydrangea, is the most economically important member of the Hydrangea genus, with a breeding history spanning hundreds of years. Bigleaf hydrangea breeding improvement has largely focused on aesthetic traits and there are few varieties tolerant or resistant to major diseases such as powdery mildew. Powdery mildew is an obligate biotrophic Ascomycete in the order Erysiphales represented by approximately 900 species worldwide. The disease-causing agent in hydrangeas is Golovinomyces orontii (formerly Erysiphe polygoni DC), which tarnishes the beauty, growth, and salability of bigleaf hydrangea plants, especially those packed closely in production environments. Chemical or biological control is commonly used in production. A recently published haplotype-resolved genome of bigleaf hydrangea enables targeted analyses and breeding techniques for powdery mildew resistance. Analyzing transcriptomes of tolerant and susceptible hydrangeas through RNA sequencing will lead to the identification of differentially expressed genes and/or pathways. Concurrent application of marker-assisted selection, genetic transformation, and gene editing will contribute to the development of powdery-mildew-resistant varieties of bigleaf hydrangea. The aim of this review is to give a general overview of powdery mildew, its impact on bigleaf hydrangea, current control methods, molecular mechanisms, and breeding prospects for powdery mildew resistance in bigleaf hydrangea.

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Section: Breeding For Powdery Mildew Resistancementioning

confidence: 99%

Section: Breeding For Powdery Mildew Resistancementioning

confidence: 99%

Powdery Mildew of Bigleaf Hydrangea: Biology, Control, and Breeding Strategies for Resistance

Jennings,

Baysal-Gurel,

Alexander

2024

Horticulturae

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“…Interspecific hybrids between H. macrophylla "Schneeball" × H. arborescens "Annabelle" via ovule culture were achieved by Cai et al [11] and the new genotypes obtained have been subjected to morphological, cytological, and molecular analysis. In vitro germination of seeds is a common technique used to overcome incompatibility barriers in ornamental plant hybrids [12][13][14][15][16]. Since most Hydrangea seeds are so small (0.5 mm diameter) and hybrid seed production is usually low (0-5 seeds/fruit), the in vitro method for Hydrangea fertilized ovules rescuing is an important tool to obtain new variability.…”

Section: Introductionmentioning

confidence: 99%

“In Vitro” Ovule Culture to Improve Genetic Variability in Hydrangea macrophylla

Nesi,

Ghiselli,

Gori

et al. 2023

Horticulturae

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In flowering plants, such as Hydrangea macrophylla, the main breeding objective is to increase genetic variability in ornamental traits. This study investigates in vitro techniques, through ovule culture, to overcome the hybridization barriers and increase the efficiency of crossing in Hydrangea macrophylla in which breeding has been hampered by a fairly long breeding cycle and lack of information about its genetic resources. Two different types of media were compared, Gamborg B5 and Murashige and Skoog basal salts, to verify the germination rate of immature ovules in different intraspecific crosses. The germination rate and viability of the seedlings were influenced by the parental genotypes in the different combinations of crossing, highlighting, in some cases, the poor compatibility between some of them. The crossing combination “Parental A × Parental B”, showed the highest germinated ovules percentage (78.3%). The media used seem to less affect the ovule germination while mainly influencing the development and growth of the young seedlings and in particular the number of leaves, the branching attitude, and root length, with the Gamborg medium determining up to a 30% increase, compared to MS medium. In addition, we tested the effectiveness of using SSR markers to assess the parentage of the putative hybrids even though only three out of twelve SSR markers showed allelism. Although the number of SSR markers was low, they were allowed to profile the parentage according to Mendelian laws.

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Development of complementary techniques applied toHydrangeahybridizations

Cited by 2 publications

References 9 publications

Powdery Mildew of Bigleaf Hydrangea: Biology, Control, and Breeding Strategies for Resistance

Powdery Mildew of Bigleaf Hydrangea: Biology, Control, and Breeding Strategies for Resistance

“In Vitro” Ovule Culture to Improve Genetic Variability in Hydrangea macrophylla

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