Development of colorimetric sensors based on gold nanoparticles for SARS-CoV-2 RdRp, E and S genes detection

Diaz, Ciro Rodriguez; Lafuente-Gómez, Nuria; Coutinho, Catarina; Pardo, Demián; Alarcón-Iniesta, Hernán; López-Valls, María; Coloma, Rocío; Milán-Rois, Paula; Domenech, Mirian; Abreu, Melanie; Galán, Juan Carlos; Bocanegra, Rebeca; Campos, Luis Alberto; Miranda, Rodolfo; Castellanos, Milagros; Somoza, Álvaro

doi:10.1016/j.talanta.2022.123393

Cited by 21 publications

(18 citation statements)

References 56 publications

(71 reference statements)

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“…In general, RNA viruses have very low concentrations of their genomes; therefore, ultrasensitive detection is necessary. In our system, LOD was 1160 copies/ml, which is similar with RT-qPCR methods such as reported by Yang M, et al and Corman et al and even highly sensitive compared to other colorimetric detection method as we mentioned in Table S2 [ [33] , [34] , [35] , [36] , [37] ].…”

Section: Resultssupporting

confidence: 84%

Multiple ligation–Assisted recombinase polymerase amplification for highly sensitive and selective colorimetric detection of SARS-CoV-2

Alam

Kumar

Lee

et al. 2023

Talanta

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Section: Resultssupporting

confidence: 84%

Multiple ligation–Assisted recombinase polymerase amplification for highly sensitive and selective colorimetric detection of SARS-CoV-2

Alam

Kumar

Lee

et al. 2023

Talanta

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“…The color changes as a result of aggregation of AuNPs can be led to the naked-eye detection of COVID-19. For example, Rodríguez-Díaz et al [ 62 ] designed the colorimetric genosensors using the molecular beacon–functionalized AuNPs for the detection of RdRP, E, and S genes. To reach this goal, both the AuNP and cholesterol molecules were attached to the two ends of the molecular beacon.…”

Section: Colorimetric Sensing Methodsmentioning

confidence: 99%

AuNP-based biosensors for the diagnosis of pathogenic human coronaviruses: COVID-19 pandemic developments

2022

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The outbreak rate of human coronaviruses (CoVs) especially highly pathogenic CoVs is increasing alarmingly. Early detection of these viruses allows treatment interventions to be provided more quickly to people at higher risk, as well as helping to identify asymptomatic carriers and isolate them as quickly as possible, thus preventing the disease transmission chain. The current diagnostic methods such as RT-PCR are not ideal due to high cost, low accuracy, low speed, and probability of false results. Therefore, a reliable and accurate method for the detection of CoVs in biofluids can become a front-line tool in order to deal with the spread of these deadly viruses. Currently, the nanomaterial-based sensing devices for detection of human coronaviruses from laboratory diagnosis to point-of-care (PoC) diagnosis are progressing rapidly. Gold nanoparticles (AuNPs) have revolutionized the field of biosensors because of the outstanding optical and electrochemical properties. In this review paper, a detailed overview of AuNP-based biosensing strategies with the varied transducers (electrochemical, optical, etc.) and also different biomarkers (protein antigens and nucleic acids) was presented for the detection of human coronaviruses including SARS-CoV-2, SARS-CoV-1, and MERS-CoV and lowly pathogenic CoVs. The present review highlights the newest trends in the SARS-CoV-2 nanobiosensors from the beginning of the COVID-19 epidemic until 2022. We hope that the presented examples in this review paper convince readers that AuNPs are a suitable platform for the designing of biosensors. Graphical abstract

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“…The qualitative and quantitative assessment was carried out using three samples: synthetic, infected cells, and patients. Moreover, medium and high viral loads (≥10 3 –10 4 viral RNA replica/μL) were estimated from patient samples by implementing the amplification tactic quickly and affordably, and the process was completed in 2.5 h. 160…”

Section: Coinage Metals and Their Antiviral Abilitymentioning

confidence: 99%

Antiviral effects of coinage metal-based nanomaterials to combat COVID-19 and its variants

2022

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Development of colorimetric sensors based on gold nanoparticles for SARS-CoV-2 RdRp, E and S genes detection

Cited by 21 publications

References 56 publications

Multiple ligation–Assisted recombinase polymerase amplification for highly sensitive and selective colorimetric detection of SARS-CoV-2

Multiple ligation–Assisted recombinase polymerase amplification for highly sensitive and selective colorimetric detection of SARS-CoV-2

AuNP-based biosensors for the diagnosis of pathogenic human coronaviruses: COVID-19 pandemic developments

Antiviral effects of coinage metal-based nanomaterials to combat COVID-19 and its variants

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