Development of colloidal gold immunochromatographic signal-amplifying system for ultrasensitive detection of Escherichia coli O157:H7 in milk

Wang, Jingyun; Chen, Ming‐Hui; Sheng, Zhi-Chao; Liu, Daofeng; Wu, Song-Song; Lai, Weihua

doi:10.1039/c5ra13279g

Cited by 57 publications

(33 citation statements)

References 16 publications

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“…In all these cases, the decrease in LOD is due to higher coloration of the formed enlarged particles in comparison with the initial seed particles. For bacteria, the LOD decrease observed was in the range from 8 ( Escherichia coli O157: H7 in milk, [51]) to 100 times (for Salmonella enteritidis in milk [29]).…”

Section: Resultsmentioning

confidence: 99%

Enlargement of Gold Nanoparticles for Sensitive Immunochromatographic Diagnostics of Potato Brown Rot

Razo

Panferova

Panferov

et al. 2019

Sensors

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Lateral flow immunoassay (LFIA) is a convenient tool for rapid field-based control of various bacterial targets. However, for many applications, the detection limits obtained by LFIA are not sufficient. In this paper, we propose enlarging gold nanoparticles’ (GNPs) size to develop a sensitive lateral flow immunoassay to detect Ralstonia solanacearum. This bacterium is a quarantine organism that causes potato brown rot. We fabricated lateral flow test strips using gold nanoparticles (17.4 ± 1.0 nm) as a label and their conjugates with antibodies specific to R. solanacearum. We proposed a signal enhancement in the test strips’ test zone due to the tetrachloroauric (III) anion reduction on the GNP surface, and the increase in size of the gold nanoparticles on the test strips was approximately up to 100 nm, as confirmed by scanning electron microscopy. Overall, the gold enhancement approach decreased the detection limit of R. solanacearum by 33 times, to as low as 3 × 104 cells∙mL–1 in the potato tuber extract. The achieved detection limit allows the diagnosis of latent infection in potato tubers. The developed approach based on gold enhancement does not complicate analyses and requires only 3 min. The developed assay together with the sample preparation and gold enlargement requires 15 min. Thus, the developed approach is promising for the development of lateral flow test strips and their subsequent introduction into diagnostic practice.

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Section: Resultsmentioning

confidence: 99%

Enlargement of Gold Nanoparticles for Sensitive Immunochromatographic Diagnostics of Potato Brown Rot

Razo

Panferova

Panferov

et al. 2019

Sensors

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“…Ten grams each of non-C. sakazakii PIF sample was homogenized in 90 mL of nutrient broth medium and C. sakazakii (6 cfu), followed by incubation at 37°C and shaking at 160 rpm (Wang et al, 2015;Shukla et al, 2016). Aliquots of 1 mL from each bottle at 3, 4.5, 6, 7.5, and 9 h incubation periods were collected.…”

Section: Detection Of C Sakazakii In Pifmentioning

confidence: 99%

Gold nanoparticle-based enhanced lateral flow immunoassay for detection of Cronobacter sakazakii in powdered infant formula

Pan¹,

Jiang²,

Sun

et al. 2018

Journal of Dairy Science

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Cronobacter sakazakii is an opportunistic foodborne pathogen that can infect newborns through powdered infant formula (PIF). In this study, we developed a novel enhanced lateral flow immunoassay (LFA) with enhanced sensitivity for detection of C. sakazakii in PIF by the naked eye. The proposed strategy for signal enhancement of the traditional LFA used concentrated gold nanoparticles (AuNP) as the enhancer to conjugate with capture antibodies, which could increase the immobilized capture antibodies concentration at the detection zone to improve capture efficiency. Besides, the detection signal was further amplified by accumulated AuNP as the C. sakazakii labeled with AuNP probes was captured by antibodies conjugated with enhancer at the test line. We also studied the effect of different concentrations of capture antibodies and concentrated AuNP on detection performance, and found that 2.2 mg/mL of capture antibodies and 0.06 nM concentrated AuNP were the optimal combination that could avoid a false-positive signal and maximally amplify the detection signal of the enhanced LFA. Using this strategy, the detection sensitivity of the enhanced LFA was 10 cfu/mL and improved 100-fold compared with traditional LFA. The strip was highly specific to C. sakazakii, and the time for detection of C. sakazakii in PIF was shortened by 3 h. In summary, the enhanced LFA developed by the addition of concentrated AuNP as the enhancer can be used as a sensitive, rapid, visual qualitative and point-of-care test method for detecting target analytes.

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“…For comparison, the CG-ICA and MNP-ICA were developed and optimized according to previous work. 19,27 The procedure of CG-ICA and MNP-ICA were conducted as follows. A total of 8 mL of ICG or 8 mg of IMNPs was added in 100 mL of a series of S. enteritidis dilutions (1.95 Â 10 3 to 1.95 Â 10 8 CFU mL À1 ), and 100 mL of the mixture was transferred into the test strip.…”

Section: Sensitivity Test Of Three Icasmentioning

confidence: 99%

Rapid and sensitive detection of Salmonella enteritidis by a pre-concentrated immunochromatographic assay in a large-volume sample system

Duan

Huang²,

et al. 2017

RSC Adv.

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Development of colloidal gold immunochromatographic signal-amplifying system for ultrasensitive detection of Escherichia coli O157:H7 in milk

Cited by 57 publications

References 16 publications

Enlargement of Gold Nanoparticles for Sensitive Immunochromatographic Diagnostics of Potato Brown Rot

Enlargement of Gold Nanoparticles for Sensitive Immunochromatographic Diagnostics of Potato Brown Rot

Gold nanoparticle-based enhanced lateral flow immunoassay for detection of Cronobacter sakazakii in powdered infant formula

Rapid and sensitive detection of Salmonella enteritidis by a pre-concentrated immunochromatographic assay in a large-volume sample system

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