Development of collateral vessels: A new paradigm in CAM angiogenesis model

Gatne, Dipti; Mungekar, Snehal; Addepalli, Veeranjaneyulu; Mohanraj, Krishnapriya; Ghone, Sanjeevani A.; Rege, Nirmala N

doi:10.1016/j.mvr.2015.09.002

Cited by 8 publications

(5 citation statements)

References 10 publications

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“…A mild suction was applied to the blunt end burr hole to displace the air sac and drop the CAM away from the shell. Fine forceps were then used to pick away the shell over the false air sac [18,27], so a square window of one cm 2 was opened in the egg shell in aseptic conditions and under laminar flow (Bioflux, Japan) and then, sealed with parafilm to prevent dehydration and the eggs were returned for incubation. On the 8th day of incubation (Day 8), the eggs were removed from the incubator [28].…”

Section: Chick Chorioallantoic Membrane (Cam) Assaymentioning

confidence: 99%

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Down-regulation of TGF-β, VEGF, and bFGF in vascular endothelial cells of chicken induced by a brittle star (Ophiocoma erinaceus) extract

Kachooei

Rahmani

Zareh

et al. 2020

Heliyon

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A B S T R A C TGreat attention has been focused on the discovery of anti-angiogenic natural and synthetic compounds to be finally used as or at least a part of the treatment of tumors. The marine ecosystems provide diversity in natural chemicals with the potential of being exploited as medicines in the treatment of diseases. Several studies have investigated Ophiuroids as a source of anti-tumor and anti-metastatic organisms. Here, we described the inhibitory effects of an ethanolic crude extract of brittle star (Ophiocoma erinaceus) on angiogenesis and the expression level of TGF-β, VEGF, and bFGF in chicken chorioallantoic membrane (CAM) as an experimental model. To do this 45 embryonated eggs were randomly divided into six groups including the control group, sham, three experimental groups and positive. The number and the length of vessels were calculated using ImageJ ® software. The relative mRNA levels of the genes in different groups were evaluated by qRT-PCR method. Our study was suggestive of an anti-angiogenesis effect of brittle star ethanolic crude extract in a CAM model. The extract also showed a pharmacological effect of down-regulation of mRNA related to VEGF, TGF-β, and bFGF genes on chicken vascular endothelial cells. It was also showed that the observed inhibitory effect is with a dose-dependent manner in which the highest inhibitory effect belonged to the highest used dose. We indicated the anti-angiogenesis properties of the Persian Gulf brittle star. Further studies are needed in other aspects of the brittle star extract in the treatment of angiogenesis, hyperplasia, and cancers.

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Section: Chick Chorioallantoic Membrane (Cam) Assaymentioning

confidence: 99%

“…Most frequently used of the methods, human amnion, air sacs, mouse cornea, and Chick Chorioallantoic Membrane (CAM) can be mentioned. Ease of use, low costs, high repeatability, has made the CAM method of choice for many angiogenesis studies [15,16,17,18].…”

Section: Introductionmentioning

confidence: 99%

Down-regulation of TGF-β, VEGF, and bFGF in vascular endothelial cells of chicken induced by a brittle star (Ophiocoma erinaceus) extract

Kachooei

Rahmani

Zareh

et al. 2020

Heliyon

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“…The surface area of the CAM increased from approximately 6 cm 2 at day 6 to 65 cm 2 by day 14. In addition to being an angiogenesis model, CAM assay has many applications, including in-vivo evaluation of drug delivery systems, tumor implantation, and toxicological studies (40)(41)(42). The main reason for our selecting CAM assay was our belief that a tissue having such an abundant vascular structure would provide us with a very good idea about the effects of drugs on angiogenesis.…”

Section: Se19mentioning

confidence: 99%

Opioids Inhibit Angiogenesis in a Chorioallantoic Membrane Model

et al. 2017

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Background: Angiogenesis is an important characteristic of cancer. Switching from the avascular phase to the vascular phase is a necessary process for tumor growth. Therefore, research in cancer treatment has focused on angiogenesis as a drug target. Despite the widespread use of opioids to treat pain in patients with cancer, little is known about the effect of these drugs on vascular endothelium and angiogenesis. Objectives: We aimed to investigate the efficacies of morphine, codeine, and tramadol in 3 different concentrations on angiogenesis in hens’ eggs. Study Design: This is a prospective, observational, controlled, in-vivo animal study. Setting: Single academic medical center. Methods: This study was conducted on the chorioallantoic membrane (CAM) of fertilized hens’ eggs. The efficacies of morphine, codeine, and tramadol in 3 different concentrations were evaluated on angiogenesis in a total of 165 hens’ eggs. Results: Statistically significant differences were found between drug-free agarose used as a negative control and concentrations of morphine of 10 µM and 1 µM, a concentration of tramadol of 10 µM, and concentrations of codeine of 10 µM and 1 µM. Concentrations of morphine of 10 µM and 1 µM showed strong antiangiogenic effects. While codeine had strong antiangiogenic effects at high concentrations, at 0.1 µM it was shown to have weak antiangiogenic effects. However, tramadol at a concentration of 10 µM had only weak antiangiogenic effects. Limitations: This is just a CAM model study. Conclusion: In this study, we tested the effects of 3 different opioid drugs on angiogenesis in 3 different concentrations, and we observed that morphine was a good anti-angiogenic agent, but tramadol and codeine only had anti-angiogenic effects at high doses. Key Words: Morphine, codeine, tramadol, opioid, bevacizumab, chorioallantoic membrane (CAM), angiogenesis

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“…2 inhibiting angiogenesis can have a beneficial role against such diseases, including cancers, macular degeneration, diabetic retinopathy, rheumatoid arthritis, and psoriasis [5].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of angiogenesis by the nutraceutical Terasen® in the chorioallantoic membrane from chicken embryo

De Melo,

Tavares,

Santos

et al. 2023

Preprint

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Angiogenesis is a process whereby new blood vessels are born from preexistent ones, which has a role in transporting oxygen and nutrients. On the other hand, inhibiting this process is a strategy used to treat some diseases, including cancers, diabetic retinopathy, and rheumatoid arthritis. This study investigates the effect of a new nutraceutical (Terasen®) on angiogenesis. Terasen® is made from a standardized extract of Euterpe oleraceae (Açaí), Myrciaria dubia (Camu-Camu), and purified oil of Bixa orellana (Annatto) and Astrocaryum aculeatum (Tucumã). The in vivo angiogenesis model used was the chorioallantoic membrane (CAM) from chicken embryos. The embryos were divided into seven groups: a vehicle control treated with ethanol solution (ETH), a naïve control treated only with saline solution (SOR), a positive control treated with dexamethasone (DEX), and four groups treated with Terasen® at different concentrations (0.5, 1, 2, and 3 mg/ml). The parameters assessed were the vessels’ area, length, and amount. The results show a statistically significant and concentration-dependent inhibition of angiogenesis caused by Terasen®. Our results suggest the nutraceutical has a high antiangiogenesis potential, possibly due to its bioactive compounds, including anthocyanins, quercetin, and tocotrienols.

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Development of collateral vessels: A new paradigm in CAM angiogenesis model

Cited by 8 publications

References 10 publications

Down-regulation of TGF-β, VEGF, and bFGF in vascular endothelial cells of chicken induced by a brittle star (Ophiocoma erinaceus) extract

Down-regulation of TGF-β, VEGF, and bFGF in vascular endothelial cells of chicken induced by a brittle star (Ophiocoma erinaceus) extract

Opioids Inhibit Angiogenesis in a Chorioallantoic Membrane Model

Inhibition of angiogenesis by the nutraceutical Terasen® in the chorioallantoic membrane from chicken embryo

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