Development of collagen/nanohydroxyapatite scaffolds containing plant extract intended for bone regeneration

Garcia, Claudio Fernandes; Marangon, Crisiane Aparecida; Massimino, Lívia Contini; Klingbeil, María Fátima Guarizo; Martins, Virgínia da Conceição Amaro; Plepis, Ana Maria de Guzzi

doi:10.1016/j.msec.2021.111955

Cited by 24 publications

(17 citation statements)

References 46 publications

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“…There is a broad consensus on the method of culturing cells with extracts from the materials to evaluate the cytotoxicity of biological implant materials. 37,38 In this study, the cytotoxicities of the MI-NHAC and NHAC scaffold extracts on MC3T3-E1 cells were assessed using the CCK-8 assay. As shown in Table 1, the relative growth rate remained above 80% in both the NHAC group and the MI-NHAC group after 1, 3 and 5 days, and the toxicity ratings were all grade 1, compounding the ISO standard.…”

Section: Resultsmentioning

confidence: 99%

Fabrication method for a magnetically induced highly oriented nanohydroxyapatite/collagen composite

Shuai

Zhou

Liu

et al. 2022

Journal of Applied Biomaterials & Functional Materials

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Both collagen fibres and nanohydroxyapatite crystals have anisotropic magnetisation, which allows them to be oriented by a high magnetic field. Highly oriented nanohydroxyapatite/collagen composites were prepared using a high magnetic field combined with in situ synthesis. These highly oriented composites were investigated and compared with conventional composites. The results showed that the collagen fibres in the magnetically induced highly oriented nanohydroxyapatite/collagen composites had a preferred orientation and smaller molecular spacing, while the nanohydroxyapatite crystals were tightly adhered along the collagen fibre surface. The magnetically induced composites exhibited superior resistance to swelling and degradation along with high compressive strength. This artificial composite, with a structure and composition similar to natural bone, represents a new idea for improving materials for vertical or horizontal bone augmentation.

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Section: Resultsmentioning

confidence: 99%

Fabrication method for a magnetically induced highly oriented nanohydroxyapatite/collagen composite

Shuai

Zhou

Liu

et al. 2022

Journal of Applied Biomaterials & Functional Materials

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“…Otherwise, ursolic acid-loaded-mesoporous hydroxylapatite-chitosan scaffolds were reported to regulate bone regeneration ability by inhibiting the polarization of macrophages to M1 type [ 59 ]. In another study, grape seed, pomegranate peel, and jabuticaba peel extracts direct blend with scaffolds of nanohydroxyapatite and collagen showed potent anti-bacterial activity and offered a promising strategy to design novel biomaterials for bone tissue regeneration [ 60 ]. In addition, scaffolds containing herbal extracts, such as Cissus quadrangularis [ 61 ], kaempferol [ 62 ], aloe vera extract [ 63 ], and Elaeagnus Angustifolia extract [ 64 ], have shown a potential bone tissue regeneration ability.…”

Section: Discussionmentioning

confidence: 99%

Notoginsenoside R1 Promotes Migration, Adhesin, Spreading, and Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stromal Cells

et al. 2022

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Cellular activities, such as attachment, spreading, proliferation, migration, and differentiation are indispensable for the success of bone tissue engineering. Mesenchymal stromal cells (MSCs) are the key precursor cells to regenerate bone. Bioactive compounds from natural products had shown bone regenerative potential. Notoginsenoside R1 (NGR1) is a primary bioactive natural compound that regulates various biological activities, including cardiovascular protection, neuro-protection, and anti-cancer effects. However, the effect of NGR1 on migration, adhesion, spreading, and osteogenic differentiation of MSCs required for bone tissue engineering application has not been tested properly. In this study, we aimed to analyze the effect of NGR1 on the cellular activities of MSCs. Since human adipose-derived stromal cells (hASCs) are commonly used MSCs for bone tissue engineering, we used hASCs as a model of MSCs. The optimal concentration of 0.05 μg/mL NGR1 was biocompatible and promoted migration and osteogenic differentiation of hASCs. Pro-angiogenic factor VEGF expression was upregulated in NGR1-treated hASCs. NGR1 enhanced the adhesion and spreading of hASCs on the bio-inert glass surface. NGR1 robustly promoted hASCs adhesion and survival in 3D-printed TCP scaffold both in vitro and in vivo. NGR1 mitigated LPS-induced expression of inflammatory markers IL-1β, IL-6, and TNF-α in hASCs as well as inhibited the RANKL/OPG expression ratio. In conclusion, the biocompatible NGR1 promoted the migration, adhesion, spreading, osteogenic differentiation, and anti-inflammatory properties of hASCs.

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“…Fruits (grape seed, pomegranate peel, jabuticaba peel) extracts have been used as crosslinkers for anionic collagen, which together with nanohydroxyapatite formed a smart scaffold with a pore size (17–230 nm) appropriate for bone growth [ 142 ]. Genipin, a natural compound from gardenia fruits, demonstrated a promising cross-linking ability for natural biopolymers (collagen, gelatin, and chitosan) and a promising biosafety profile.…”

Section: The Innovative Administration and Application Of Plant Extra...mentioning

confidence: 99%

Phytochemical Compounds Involved in the Bone Regeneration Process and Their Innovative Administration: A Systematic Review

Hanga-Farcaș

Miere

Filip

et al. 2023

Plants

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Bone metabolism is a complex process which is influenced by the activity of bone cells (e.g., osteocytes, osteoblasts, osteoclasts); the effect of some specific biomarkers (e.g., parathyroid hormone, vitamin D, alkaline phosphatase, osteocalcin, osteopontin, osteoprotegerin, osterix, RANKL, Runx2); and the characteristic signaling pathways (e.g., RANKL/RANK, Wnt/β, Notch, BMP, SMAD). Some phytochemical compounds—such as flavonoids, tannins, polyphenols, anthocyanins, terpenoids, polysaccharides, alkaloids and others—presented a beneficial and stimulating effect in the bone regeneration process due to the pro-estrogenic activity, the antioxidant and the anti-inflammatory effect and modulation of bone signaling pathways. Lately, nanomedicine has emerged as an innovative concept for new treatments in bone-related pathologies envisaged through the incorporation of medicinal substances in nanometric systems for oral or local administration, as well as in nanostructured scaffolds with huge potential in bone tissue engineering.

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Development of collagen/nanohydroxyapatite scaffolds containing plant extract intended for bone regeneration

Cited by 24 publications

References 46 publications

Fabrication method for a magnetically induced highly oriented nanohydroxyapatite/collagen composite

Fabrication method for a magnetically induced highly oriented nanohydroxyapatite/collagen composite

Notoginsenoside R1 Promotes Migration, Adhesin, Spreading, and Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stromal Cells

Phytochemical Compounds Involved in the Bone Regeneration Process and Their Innovative Administration: A Systematic Review

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