Development of Coagulation Regulatory Proteins in the Fetal and Neonatal Lamb

Manco‐Johnson, Marilyn J.; Jacobson, Linda; Hacker, Michele R.; Townsend, Susan F.; Murphy, James M.; Hay, William W.

doi:10.1203/00006450-200210000-00019

Cited by 15 publications

(7 citation statements)

References 52 publications

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“…Examination of hemostatic function in sheep is primarily important with respect to their role as an animal model of various human diseases and procedures, including surgical methods, 9 surgical trauma, 10–12 cardiovascular surgery, 13–15 sepsis, 16 and shock 17 . However, only a few studies have been published regarding the methods used to investigate primary hemostasis in ovine blood, 18–23 and only one of these utilizes whole blood aggregometry 23 …”

Section: Introductionmentioning

confidence: 99%

Measurement of platelet aggregation in ovine blood using a new impedance aggregometer

Baumgarten

Wilhelmi

Kalbantner

et al. 2010

Veterinary Clinical Pathol

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Background: Whole blood platelet aggregometry (impedance) is an important method to investigate platelet function disorders. Examination of hemostatic function in sheep is important with respect to their role as an animal model of human disease. Objective: The aim of this study was to evaluate and optimize selected methodological aspects (anticoagulant, agonist concentration) of impedance aggregometry in ovine blood using the new Multiplate 5.0 analyzer. Methods: Blood samples were collected in hirudin anticoagulant from 40 clinically healthy sheep. Samples from selected sheep were collected in citrate, with or without the addition of calcium chloride. The agonists adenosine diphosphate (ADP), collagen, ristocetin, arachidonic acid, and thrombin receptor-activating peptide (TRAP) were added in several concentrations to induce aggregation. Results: Based on maximum aggregation values and internal precision, no significant difference was found between ADP concentrations of 3-10 mmol/L and collagen concentrations of 3-5 mg/mL (P 4.05). The lowest interindividual variation of approximately 3-4-fold was seen with 4 and 5 mmol/L ADP and 4 and 5 mg/mL collagen. Ristocetin, arachidonic acid, and TRAP did not induce significant aggregation at any concentration. Aggregation results were significantly lower when measured in citrate-vs hirudin-anticoagulated blood, regardless of the presence of calcium chloride. Conclusions: Our results indicate that the multiplate impedance aggregometer is suitable for the measurement of platelet aggregation in sheep using optimal agonist concentrations of 4-5 mmol/L ADP and 4-5 mg/mL collagen. Hirudin-anticoagulated blood is the preferred sample material.

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Section: Introductionmentioning

confidence: 99%

Measurement of platelet aggregation in ovine blood using a new impedance aggregometer

Baumgarten

Wilhelmi

Kalbantner

et al. 2010

Veterinary Clinical Pathol

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“…We essentially used TargetScan [17] (release 5.1: http://www.targetscan.org), which provides the prediction results computed by the TargetScanS algorithm, PicTar ( http://www.pictar.mdc-berlin.de) [18], and miRanda ( http://www.microrna.org/microrna/home.do) [19]. …”

Section: Methodsmentioning

confidence: 99%

Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a

et al. 2013

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BackgroundDevelopmental haemostatic studies may help identifying new elements involved in the control of key haemostatic proteins like antithrombin, the most relevant endogenous anticoagulant.ResultsIn this study, we showed a significant reduction of sialic acid content in neonatal antithrombin compared with adult antithrombin in mice. mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, were 85% lower in neonates in comparison with adults. In silico analysis of miRNAs overexpressed in neonates revealed that mir-200a might target these sialyltransferases. Moreover, in vitro studies in murine primary hepatocytes sustain this potential control.ConclusionsThese data suggest that in addition to the direct protein regulation, microRNAs may also modulate qualitative traits of selected proteins by an indirect control of post-translational processes.

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“…The intrauterine development of PC was explored in ovine models and reported in 1995 by Manco-Johnson et al, 16 who continued to study the fetal development of coagulation regulatory proteins during the subsequent decade. 17 These workers demonstrated that vitamin K-dependent coagulation proteins are characterized by early appearance in plasma despite their delayed fetal maturation. By mid-gestation, factors II, IX, X, PC, and protein S are at 10 to 20% of adult levels whereas factor VII is slightly higher.…”

Section: Introduction: the History Of Developmental Hemostasismentioning

confidence: 99%

50 Years of Pediatric Hemostasis: Knowledge, Diagnosis, and Treatment

Levy‐Mendelovich

Cohen

Klang

et al. 2022

Semin Thromb Hemost

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Studies from the past 50 years have contributed to the expanding knowledge regarding developmental hemostasis. This is a dynamic process that begins in the fetal phase and is characterized by physiological variations in platelet counts and function, and concentrations of most coagulation factors and the native coagulation inhibitors in early life, as compared with adulthood. The developmental hemostasis studies since the 1980 to 1990s established the laboratory reference values for coagulation factors. It was only a decade or two later, that thromboelastography (TEG) or (rotational thromboelastometry [ROTEM]) as well as thrombin generation studies, provided special pediatric reference values along with the ability to evaluate clot formation and lysis. In addition, global whole blood-based clotting assays provided point of care guidance for proper transfusion support to children hospitalized in intensive care units or undergoing surgery. Although uncommon, thrombosis in children and neonates is gaining increasing recognition, typically as a secondary complication in sick children. Bleeding in children, and particularly intracerebral hemorrhage in newborns, still represent a therapeutic challenge. Notably, our review will outline the advancements in understanding developmental hemostasis and its manifestations, with respect to the pathophysiology of thrombosis and bleeding complications in young children. The changes of transfusion policy and approach to thrombophilia testing during the last decade will be mentioned. Subsequently, a brief summary of the data on anticoagulant treatments in pediatric patients will be presented. Finally, we will point out the 10 most cited articles in the field of pediatric and neonatal hemostasis.

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Development of Coagulation Regulatory Proteins in the Fetal and Neonatal Lamb

Cited by 15 publications

References 52 publications

Measurement of platelet aggregation in ovine blood using a new impedance aggregometer

Measurement of platelet aggregation in ovine blood using a new impedance aggregometer

Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a

50 Years of Pediatric Hemostasis: Knowledge, Diagnosis, and Treatment

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