Development of Clonal Micropropagation Technology for Ludisia Discolor (Ker Gawl.) A. Rich. In Vitro Conditions

Abstract

The features of clonal micropropagation in three variations of Ludisia discolor were studied. Brood buds, apical shoot meristems, immature seeds and mature seeds were used as explants. They are grown using Murashige & Skoog modified medium. A special technique has been developed for the sterilization and planting of microscopic mature seeds. Mature seeds germinated for 60-70 days. Seeds from 25-day-old capsules showed the best results among immature seeds. They gave mass shoots already on the 25-30 day after planting. The roller of the future first leaf of the protocorms was laid in the dark 2 months after germination. Switching to the light mode induced rapid gemmorizogenesis - the formation of the first green leaves, stem and adventitious roots. Seeds from 15-day-old capsules did not germinate at all. Planting with immature seeds is the most effective method of clonal reproduction of L. discolor in vitro.

Use of landscapes near Tudakul reservoir eficiently

“…In general, the average speed of winds in the Kuyimozor-Tudakul region is somewhat higher than in the southern and central parts of the region. [12].…”

“…Effective use of the above-mentioned nutritious plants, their reproduction and protection will allow to increase the productivity of the available pastures in the region, and this is one of the promising ways to develop pastoralism. [12] In the past, few people lived in Tudakul region, only a small number of shepherds lived and raised livestock. Wild animals such as wolves, foxes, rabbits and gazelles lived around the Tudakul reservoir, and it should be noted that the four sides of the reservoir consist of mountains, groves, and ditches, which are important factors that allow wild animals to Tudakul is a comfortable and unique place for pheasants, mallards, ducks and various other birds in its groves and around water.…”