Development of classical least square method for the determination of Candesartan and Hydrochlorthiazide in tablet dosage form

Daharwal, S. J.; Singh, Veena D.

doi:10.5958/2231-5691.2015.00013.1

Cited by 5 publications

(3 citation statements)

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“…RE method is advantageous over both AR and PR methods since it has no limitation in the choice of two wavelengths used in signals analysis with no need for the presence of extension region or iso-point, accordingly it could be applied on all mixtures containing different drugs concentrations with satisfactory results. This unique data processing technique offer advantages over the other reported spectrophotometric methods ( Abdelaleem et al, 2013 ; Ahmad Charoo et al, 2009 ; Belal et al, 2013 ; Daharwal and Singh, 2015 ; H Mohamed et al, 2020 ; Mukthinuthalapati and Kumar, 2015 ; Üstündağ and Dinç, 2021 ; Workie et al, 2017 ; Youssef et al, 2010 ; ÜSTÜNDAĞ and Erdal, 2021 ;; Erk, 2003a ;) where it encouraged the extraction of the parent spectra of each drug in the mixture acting as spectral print and confirming their purities.…”

Section: Resultsmentioning

confidence: 98%

“…For effective pharmacological action of the proposed drugs, they are co-formulated in single-pill (Atacand plus® tablet), these double antihypertensive medicines attract the attention of researchers due to their paired pharmacological effects including the drug's capability to reduce levels of the blood pressure (BP) as well as lowering risk of coronavirus mortality and morbidity of antihypertensive patients. Different analytical methods have been reported for the studied binary mixture of CAN and HCT comprising spectrophotometry ( Abdelaleem et al, 2013 ; Ahmad Charoo et al, 2009 ; Belal et al, 2013 ; Daharwal and Singh, 2015 ; H Mohamed et al, 2020 ; Mukthinuthalapati and Kumar, 2015 ; Üstündağ and Dinç, 2021 ; Workie et al, 2017 ; Youssef et al, 2010 ; ÜSTÜNDAĞ and Erdal, 2021 ÜSTÜNDAĞ and Erdal, 2021 ;; Erk, 2003a ÜSTÜNDAĞ and Erdal, 2021;) and chromatography methods( Bharathi et al, 2012 ; Bonthu et al, 2018 ; de Diego et al, 2018 de Diego et al, 2018 ;; Erk, 2003b ; Khedr, 2008 Khedr, 2008 ;; Madhavi et al, 2017 ; Singh et al, 2014 ; Üstündağ and Dinç, 2021 ; Youssef et al, 2010 ; Gaurkhede and Chandewar, 2018 ; Pappula et al, 2019 de Diego et al, 2018;Khedr, 2008;).…”

Section: Introductionmentioning

confidence: 99%

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Sustainable spectrophotometric determination of antihypertensive medicines reducing COVID-19 risk via paired wavelength data processing technique - Assessment of purity, greenness and whiteness

El-Hanboushy

Marzouk

Fayez

et al. 2022

Sustainable Chemistry and Pharmacy

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Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Sustainable spectrophotometric determination of antihypertensive medicines reducing COVID-19 risk via paired wavelength data processing technique - Assessment of purity, greenness and whiteness

El-Hanboushy

Marzouk

Fayez

et al. 2022

Sustainable Chemistry and Pharmacy

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“…Several chemometric methods have also been developed for its quantification, including Fourier Functions [24], classical least square [25], Q-analysis [26], derivative spectrophotometry [27]. It has also been determined by LC/MS/MS in human plasma [28].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Rp-HPLC Method Versus Fourier Transform Convolution Chemometric Methods; An Application on Pharmaceutical Binary Mixtures of Candesartan Cilexetil-Pitavastatin Calcium and Clopidogrel Bisulfate-Rosuvastatin Calcium

Jamal

Gazy

2016

Int J Pharm Pharm Sci

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Objective: A comparative study of smart spectrophotometric chemometric assisted techniques and RP-HPLC for the determination of candesartan cilexetil (CAN)-pitavastatin calcium (PIT) and clopidogrel bisulfate (CLO)-rosuvastatin calcium (ROS), binary co-administered drugs were developed and validated. Methods:The spectrophotometric chemometric assisted methods included two simple techniques, namely Fourier transform convolution (FF) and ratio spectra of Fourier transform convolution (FFR) methods. FFR is considered as a hybrid divisor ratio spectra method where Fourier functions are applied to divisor ratio signals. The RP-HPLC method involves a rapid separation on a C18 column using a mobile phase consisting of acetonitrile: sodium dihydrogen phosphate (adjusted to pH 2.6 using orthophosphoric acid) in the ratio of 70:30 v/v at a flow rate of 1 ml/min in isocratic mode. CLO and ROS were monitored at 220 nm however CAN and PIT were monitored at 238 nm. Results:The spectrophotometric chemometric assisted methods proved their ability to quantify each of the studied drugs in their binary mixtures, where excellent percentage recoveries were obtained. FF and FFR method proved to be linear over the concentration range of 10-50 µg/ml for CLO, 4-20 µg/ml for ROS, 8-20 µg/ml for CAN and 2-10 µg/ml for PIT. The RP-HPLC method was able to separate the drugs in the study; retention times were found to be 3.9 min and 14.4 min for ROS-CLO, 4.2 min and 14.5 min for PIT-CAN respectively. The RP-HPLC method was found to be linear in the concentration range of 0.1-0.5 µg/ml for CLO, 0.04-0.2 µg/ml for ROS, 0.5-1 µg/ml for CAN and 0.05-0.1 µg/ml for PIT. System suitability parameters proved that peaks were well resolved from each other. Conclusion:The spectrophotometric and chromatographic methods were validated according to ICH guidelines. Recovery was found to be in the range of 95.9 %-100.5 % in synthetic laboratory mixtures. The suggested spectrophotometric methods have the advantage over other methods that they do not require a preliminary separation. Statistical analysis between the suggested spectrophotometric chemometric assisted and RP-HPLC methods, using student's t-and F-test revealed that there is no difference between the applied methods.

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Validated RP-HPLC Method for the Estimation of Amiloride and Hydrochlorothiazide in Combined Tablet Dosage Form

Kumar¹,

Babu²,

Sowjanya³

et al. 2021

AJRC

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The aim of this work is to build up a rapid, exact, precise and accurate reverse phase liquid chromatographic method for the simultaneous analysis of amiloride and hydrochlorothiazide in tablet dose structure. The chromatographic strategy was normalized utilizing Hypersil ODS coulmn (250×4.6mm, 5μm molecule size) with UV detection at 210nm and flow rate of 1ml/min. The mobile phase includes phosphate buffer (pH acclimated to 2.5 with dilute Ortho Phosphoric acid) and acetonitrile in the proportion of 60:40 v/v. The linearity of proposed technique was found in the range of 5-30μg/ml (R²=0.999) for amiloride and 50-300μg/ml (R²=0.999) for Hydrochlorothiazide appropriately. The limit of detection (LOD) was discovered to be 0.10μg/ml and 0.40μg/ml for Amiloride and Hydrochlorothiazide appropriately. The limit of quantitation (LOQ) was discovered to be 0.30μg/ml and 1.20μg/ml for Amiloride and Hydrochlorothiazide separately. The retention times of Amiloride and Hydrochlorothiazide were found to be 3.258min and 2.383min separately. The technique was truly recommended and %RSD was found to be under 2 demonstrating high degree of exactness and accuracy. Subsequently proposed strategy can be effectively evaluated for the simultaneous estimation of Amiloride and Hydrochlorothiazide in promoted formulations.

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Development of classical least square method for the determination of Candesartan and Hydrochlorthiazide in tablet dosage form

Cited by 5 publications

References 0 publications

Sustainable spectrophotometric determination of antihypertensive medicines reducing COVID-19 risk via paired wavelength data processing technique - Assessment of purity, greenness and whiteness

Sustainable spectrophotometric determination of antihypertensive medicines reducing COVID-19 risk via paired wavelength data processing technique - Assessment of purity, greenness and whiteness

Comparative Study of Rp-HPLC Method Versus Fourier Transform Convolution Chemometric Methods; An Application on Pharmaceutical Binary Mixtures of Candesartan Cilexetil-Pitavastatin Calcium and Clopidogrel Bisulfate-Rosuvastatin Calcium

Validated RP-HPLC Method for the Estimation of Amiloride and Hydrochlorothiazide in Combined Tablet Dosage Form

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