Development of Class IIa Bacteriocins as Therapeutic Agents

Lohans, Christopher T.; Vederas, John C.

doi:10.1155/2012/386410

Cited by 72 publications

(72 citation statements)

References 117 publications

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“…In the media where every salt was tested separately, the best results were achieved with KCl, MgCl 2 or MnSO 4 , the activity in supernatant during the growth was increased up to 160 AU/ml. The same results were observed in the medium that was supplemented with all 8 salts or in After testing all salts and combinations of it in this work, the highest bacteriocin activity was achieved in media supplemented combining KCl and MgCl 2 or CaCl 2 and ZnSO 4 or MnSO 4 and NaHCO 3 . In this case the highest activity during the growth in these modified media was increased up to 320 AU/ml.…”

Section: Growth Medium Optimization For Higher Cell Growth and Bactersupporting

confidence: 82%

“…Combination of MgCl 2 with KCl can be even better that using MgCl 2 only. The best growth was achieved in NB medium supplemented only with MnSO 4 . Growth media components were identified that may increase better bacteriocin production in Geobacillus stearothermophilus 15 strain.…”

Section: Discussionmentioning

confidence: 99%

“…Growth media components were identified that may increase better bacteriocin production in Geobacillus stearothermophilus 15 strain. Evaluation of bacteriocin activity in media supplemented with every salt separately shows that activity may be enhanced using KCl, MgCl 2 or MnSO 4 . The best results can be achieved combining two salts: KCl and MgCl 2 , CaCl 2 and ZnSO 4 or MnSO 4 and NaHCO 3 .…”

Section: Discussionmentioning

confidence: 99%

“…The advantages of bacteriocins are their physical stability and non-toxicity. Numerous bacteriocins have great potential as food preservatives or as therapeutic or biocontrolling agents [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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Medium Optimization for Bacteriocin Production and Bacterial Cell Growth of Geobacillus sp. 15 Strain

Kaunietis¹,

Pranckutė²,

Lastauskienė³

et al. 2017

J Antimicrob Agents

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In this study we determined that Geobacillus sp. 15 strain secretes antibacterial compound-bacteriocin. Only few bacteriocin producing thermophilic bacteria of this genus have been identified to the date. Also, we optimized medium composition for the better cell growth of this strain and increased the yield of secreted bacteriocin. In this work we showed that some particular salts or their combinations may have impact on higher growth of Geobacillus sp. 15 strain bacteria or their produced bacteriocins. These results can facilitate research on this strain and its secreted bacteriocin. These antibacterial proteins and peptides are promising natural agents as an alternative to antibiotics in medicine or veterinary and to traditional preservatives in food industry. Optimized composition of the growth medium can be very useful for studies of other Geobacillus spp. strains and their produced bacteriocins. Furthermore, these data may be used to increase the biomass of Geobacillus bacteria and the yield of protein when it is dependent on bacterial cell yield.

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Section: Growth Medium Optimization For Higher Cell Growth and Bactersupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Medium Optimization for Bacteriocin Production and Bacterial Cell Growth of Geobacillus sp. 15 Strain

Kaunietis¹,

Pranckutė²,

Lastauskienė³

et al. 2017

J Antimicrob Agents

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“…Although a variety of administration methods have been proven successful, such as subcutaneous, intravenous, intranasal, intragastric, intraperitoneal, and topical, their efficacies have not yet been directly compared (Jabes et al 2011;Lohans & Vederas 2012). Moreover, some of these methods are unnecessarily invasive (Lohans & Vederas 2012).…”

Section: Bacteriocin Application and Prospectsmentioning

confidence: 99%

Bacteriocins from Lactic Acid Bacteria: A Review of Biosynthesis, Mode of Action, Fermentative Production, Uses, and Prospects

Pérez¹,

Perez²,

Elegado³

2015

PhilSciTech

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Abstract-Bacteriocins are antimicrobial peptides that help bacteria fight competing bacteria in microecological systems. Bacteriocins of lactic acid bacteria (LAB) have attracted much interest in recent years because of their properties that make them suitable as natural food preservatives against specific food pathogens, and as possible supplement to antibiotics against drug resistant bacterial strains. LAB bacteriocins are generally classified into the lantibiotics and non-lantibiotics, the latter divided into four subgroups. To date, only nisin and to a lesser extent, pediocin are the commercially applied bacteriocins for food use. Clinical applications are still limited to animal health. One of the more exciting prospects on the use of bacteriocins is the possibility of subjecting them to bioengineering to either increase antimicrobial activity or further specify their target microorganism. The latter would make it less damaging to the natural gut microflora, which is a common drawback of conventional antibiotic therapy. This paper focuses on the nature, biology, and applications of bacteriocins based on knowledge gained abroad and in the Philippines during the last two decades.

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Identification and three‐dimensional structure of carnobacteriocin XY, a class IIb bacteriocin produced by Carnobacteria

et al. 2017

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In this study, we report that CbnX (33 residues) and CbnY (29 residues) comprise a class IIb (two-component) bacteriocin in Carnobacteria. Individually, CbnX and CbnY are inactive, but together act synergistically to exert a narrow spectrum of activity. The structures of CbnX and CbnY in structure-inducing conditions were determined and strongly resemble other class IIb bacteriocins (i.e., LcnG, PlnEF, PlnJK). CbnX has an extended, amphipathic α-helix and a flexible C terminus. CbnY has two α-helices (one hydrophobic, one amphipathic) connected by a short loop and a cationic C terminus. CbnX and CbnY do not appear to interact directly and likely require a membrane-bound receptor to facilitate formation of the bacteriocin complex. This is the first class IIb bacteriocin reported for Carnobacteria.

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Development of Class IIa Bacteriocins as Therapeutic Agents

Cited by 72 publications

References 117 publications

Medium Optimization for Bacteriocin Production and Bacterial Cell Growth of Geobacillus sp. 15 Strain

Medium Optimization for Bacteriocin Production and Bacterial Cell Growth of Geobacillus sp. 15 Strain

Bacteriocins from Lactic Acid Bacteria: A Review of Biosynthesis, Mode of Action, Fermentative Production, Uses, and Prospects

Identification and three‐dimensional structure of carnobacteriocin XY, a class IIb bacteriocin produced by Carnobacteria

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