Development of chronic cardiomyopathy in canine Chagas disease correlates with high IFN-γ, TNF-α, and low IL-10 production during the acute infection phase

Guedes, Paulo Marcos da Matta; Veloso, Vanja Maria; Afonso, Luís; Caliari, Marcelo Vidigal; Carneiro, Cláudia Martins; Diniz, Lívia de Figueiredo; Silva, Eduardo de Almeida Marques da; Caldas, Ivo Santana; Matta, Maria Adelaide do Valle; Souza, Sheler Martins de; Lana, Marta de; Chiari, Égler; Galvão, Lúcia Maria da Cunha; Bahia, Maria T.

doi:10.1016/j.vetimm.2009.01.004

Cited by 70 publications

(83 citation statements)

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“…High levels of TNF produced during the acute phase of experimental T. cruzi infection may interfere with myelopoiesis, erythropoiesis, lymphopoiesis and thrombocytopoiesis (ULICH et al, 1990). We demonstrated previously that dogs infected with the ABC, Be-78 and Y strains produce high levels of IFN-γ and TNF (GUEDES et al, 2009). During the acute phase of Chagas disease, polyclonal activation of B and T lymphocytes has been observed (MINOPRIO et al, 1986), while in the present study we observed that infected dogs presented leukocytosis at 2 and 4 w.p.i.…”

Section: Discussionsupporting

confidence: 60%

“…However, the short half-life of this model interferes with reproduction of all the clinical forms of the disease. The canine model reproduces diffuse chronic myocarditis, as shown by electrocardiographic changes, along with cytokine, chemokine and antibody production with patterns similar to those found in humans (ANDRADE et al, 1981;ANSELMI et al, 1967;LANA et al, 1992;GUEDES et al, 2009;GUEDES et al, 2008;GUEDES et al, 2010).…”

Section: Introductionmentioning

confidence: 58%

“…We have previously shown that Beagle dogs are a good model for Chagas disease because they reproduce the clinical signs, parasitemia, immune responses and cardiac lesions (GUEDES et al, 2007(GUEDES et al, , 2008(GUEDES et al, , 2009(GUEDES et al, , 2010 in a manner that is very similar to what is observed in humans. Our findings indicate that the alterations in blood cell counts observed during the acute phase of experimental T. cruzi infection in Beagle dogs are associated with parasitemia levels and corroborate our previous reports.…”

Section: Discussionmentioning

confidence: 85%

“…The animals infected with the Y and ABC strains showed high parasitemia levels and all presented cardiomegaly, while the dogs infected with Be-78 showed low parasitemia levels and only 50% displayed cardiomegaly (GUEDES et al, 2008). Dogs that showed cardiomegaly had higher rates of cardiac inflammation and fibrosis, and the development of chronic cardiomyopathy was correlated with high IFN-γ, TNF-α, type-1 chemokines and low IL-10 production (GUEDES et al, 2009(GUEDES et al, , 2010.…”

Section: Discussionmentioning

confidence: 97%

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Hematological alterations during experimental canine infection by Trypanosoma cruzi

Guedes

Veloso

Mineo

et al. 2012

Rev. Bras. Parasitol. Vet.

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To confirm that Beagle dogs are a good experimental model for Chagas disease, we evaluated hematological alterations during the acute and chronic phases in Beagle dogs infected with the Y, Berenice-78 (Be-78) and ABC strains of Trypanosoma cruzi, correlating clinical signs with the parasitemia curve. We demonstrate that the acute phase of infection was marked by lethargy and loss of appetite. Simultaneously, we observed anemia, leukocytosis and lymphocytosis. Also,we describe hematological alterations and clinical signs that were positively correlated with the parasitemia during the experimental infection with the three strains of T. cruzi, and demonstrate that experimental infection of Beagle is a trustworthy model for Chagas disease.Keywords: Trypanosoma cruzi, Beagle dogs, parasitemia, lymphocytosis, anemia, leukocytosis. ResumoPara confirmar que cães Beagle são um bom modelo para doença de Chagas, foram avaliadas as alterações hematológicas durante as fases aguda e crônica em cães Beagle infectados com as cepas Y, Berenice-78 (Be-78) e ABC de Trypanosoma cruzi, correlacionando os sinais clínicos com a curva de parasitemia. Foi demonstrado que a fase aguda da infecção foi marcada por letargia e perda de apetite. Simultaneamente, observou-se anemia, leucocitose e linfocitose. Ainda, foram descritas alterações hematológicas e sinais clínicos positivamente correlacionados com a parasitemia durante a infecção experimental com as três cepas de T. cruzi estudadas, demonstrando que a infecção em cães Beagle constitui um modelo fidedigno para a doença de Chagas.

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Section: Discussionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 97%

See 2 more Smart Citations

Hematological alterations during experimental canine infection by Trypanosoma cruzi

Guedes

Veloso

Mineo

et al. 2012

Rev. Bras. Parasitol. Vet.

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“…The detection of T. cruzi in the spleen was observed previously in infected mice with abundant parasitism of macrophages in red and white pulps, hyperplasia of lymphoid follicles, and necrosis. 47 Splenomegaly is also an important characteristic of T. cruzi infection in dogs during the acute and chronic phase and it was also reported in patients with Chagas disease, 48,49 but our model did not show this macroscopic alteration.…”

Section: Discussionmentioning

confidence: 48%

Domestic Pig (Sus scrofa) as an Animal Model for Experimental Trypanosoma cruzi Infection

Yauri

Castro-Sesquen

Verástegui

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Pigs were infected with a Bolivian strain of Trypanosoma cruzi (genotype I) and evaluated up to 150 days postinoculation (dpi) to determine the use of pigs as an animal model of Chagas disease. Parasitemia was observed in the infected pigs during the acute phase (15-40 dpi). Anti-T.cruzi immunoglobulin M was detected during 15-75 dpi; high levels of anti-T.cruzi immunoglobulin G were detected in all infected pigs from 75 to 150 dpi. Parasitic DNA was observed by western blot (58%, 28/48) and polymerase chain reaction (27%, 13/48) in urine samples, and in the brain (75%, 3/4), spleen (50%, 2/4), and duodenum (25%, 1/4), but no parasitic DNA was found in the heart, colon, and kidney. Parasites were not observed microscopically in tissues samples, but mild inflammation, vasculitis, and congestion was observed in heart, brain, kidney, and spleen. This pig model was useful for the standardization of the urine test because of the higher volume that can be obtained as compared with other small animal models. However, further experiments are required to observe pathological changes characteristic of Chagas disease in humans.

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The role of fat on cardiomyopathy outcome in mouse models of chronic Trypanosoma cruzi infection

et al. 2020

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Development of chronic cardiomyopathy in canine Chagas disease correlates with high IFN-γ, TNF-α, and low IL-10 production during the acute infection phase

Cited by 70 publications

References 40 publications

Hematological alterations during experimental canine infection by Trypanosoma cruzi

Hematological alterations during experimental canine infection by Trypanosoma cruzi

Domestic Pig (Sus scrofa) as an Animal Model for Experimental Trypanosoma cruzi Infection

The role of fat on cardiomyopathy outcome in mouse models of chronic Trypanosoma cruzi infection

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