Development of Chromosome Segment Substitution Lines Derived from Backcross between indica Donor Rice Cultivar 'Nona Bokra' and japonica Recipient Cultivar 'Koshihikari'

【Short Report】Identification of Chromosome Regions Affecting Leaf Area with Rice Chromosome Segment Substitution Lines

“…We identified chromosome regions affecting a trait (CRATs) for leaf area, one of the main factors determining source ability with two sets of chromosome segment substitution lines (CSSLs) developed from indica landraces Kasalath or Nona Bokra as a donor on the genetic background of a japonica cultivar Koshihikari (Ebitani et al, 2005;Takai et al, 2007). Additionally, we confirmed their effect on source ability.…”

“…CRATs were determined by comparing phenotypes and genotypes of restriction fragment length polymorphism (RFLP: Ko/Ka CSSLs) and simple sequence repeat (SSR: Ko/NB CSSLs) markers (Ebitani et al, 2005;Takai et al, 2007). A probability level of 0.05 was used as the threshold for detection of a CRAT using the method of Madoka et al (2008) and Ujiie et al (2012).…”

“…In 2007 and 2010, heading dates of SL-LA7-2 and SL-LA11-2 were the same as that of Koshihikari (within ±3 days; data not shown). Ebitani et al (2005) and SSR markers by Takai et al (2007), a probability level of 0.05 was used as the threshold for the detection of a CRAT. b CRAT-dependent changes expressed as percentage change of the value determined in Koshihikari.…”

Low Metabolite Cost and Air-Tight Leaf Structure Regulated by Multiple Loci Enhance Rice Biomass

“…In order to characterize genetic loci that regulate higher biomass in Nana Bokra and Kasalath, biomass of CSSLs (chromosome segment substitution lines) between Koshihikari and Nona Bokra ( Figure 1D), or BILs (backcross inbred lines) between Koshihikari and Kasalath ( Figure 1E) were measured. As a result of repetitive crosses and genetic selection, only small portion of whole Koshihikari genome is substituted by genomes of Nona Bokra or Kasalath in CSSL and BIL lines (Takai et al 2007;Ma et al 2002). Against expectation, plant biomass of all CSSL lines was similar to Koshihikari, and no CSSL line had large biomass like Nona Bokra.…”

Integration of Genomics into Rice Breeding

“…To overcome problems associated with the limited detection power of conventional genetic mapping populations, researchers have developed chromosome segment substitution lines (CSSLs; Ebitani et al 2005;Takai et al 2007;Ando et al 2008;Hori et al 2010). In these novel plant materials, a particular chromosomal region in the recurrent parent is replaced by the corresponding segment from a donor genotype.…”