Development of cell line from the testicular tissues of crab Scylla serrata

Shashikumar, Anumol; Desai, Priti

doi:10.1007/s10616-011-9365-6

Cited by 16 publications

(4 citation statements)

References 20 publications

(25 reference statements)

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“…Incubation of MOs/YOs in culture medium resulted in MF/ecdysteroid secretion in a timedependent manner up to 48 h, whereas incubation of glands up to 72 h resulted in no further increase in secretion (Nagaraju et al, 2006;Reddy and Reddy, 2006). Hence, we restricted our in vitro studies to 48 h. The culture medium for S. serrata glands was prepared by adding Leibovitz's medium+crab saline (1:1), in which survivability and proliferation of testicular cells of S. serrata was at a maximum (Shashikumar and Desai, 2011). Leibovitz L-15 medium with L-glutamine powder purchased from Thermo Fisher Scientific was used.…”

Section: Determination Of Secretory Rates Of Mos and Yosmentioning

confidence: 99%

Induction of vitellogenesis, MF synthesis and ecdysteroidogenesis in two edible crabs by arachidonic acid and prostaglandins

Swetha

Girish

Hemalatha

et al. 2020

Journal of Experimental Biology

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The present study investigated the effect of arachidonic acid (AA) and selected prostaglandins on the regulation of vitellogenesis, ecdysteroidogenesis and methyl farnesoate (MF) synthesis in the freshwater crab Oziotelphusa senex senex and the giant mud crab, Scylla serrata. Administration of AA and prostaglandin F 2α (PGF 2α) and prostaglandin E 2 (PGE 2) significantly increased ovarian index, oocyte diameter and ovarian vitellogenin levels and ecdysteroid and MF levels in the hemolymph of crabs. Secretions of MF and ecdysteroids from in vitro cultured mandibular organs (MO) and Y-organs (YO) isolated from intermolt crabs injected with AA, PGF 2α and PGE 2 were greater when compared with controls. In contrast, injection of prostaglandin D 2 (PGD 2) had no effect on vitellogenesis, ecdysteroid and MF levels in circulation. In vitro secretion of MF from MO explants isolated from avitellogenic crabs incubated with AA, PGF 2α and PGE 2 increased in a time-dependent manner. Conversely, incubation of YOs isolated from avitellogenic crabs with AA, PGF 2α and PGE 2 had no effect on secretion of ecdsyteroids. These results implicate prostaglandins in the regulation of reproduction by inducing the synthesis of MF and consequent ecdysteroid synthesis in brachyuran crabs, and provide an alternative molecular intervention mechanism to the traditional eyestalk ablation methodology to induce vitellogenesis and ovarian maturation in crustaceans.

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Section: Determination Of Secretory Rates Of Mos and Yosmentioning

confidence: 99%

Induction of vitellogenesis, MF synthesis and ecdysteroidogenesis in two edible crabs by arachidonic acid and prostaglandins

Swetha

Girish

Hemalatha

et al. 2020

Journal of Experimental Biology

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“…Cell line from testes of S. serrata (Forskal) was established according to the method described by Shashikumar and Desai (2011). Briefly, the testes were removed, transferred to a flask containing sterile artificial sea water having 0.3 % antibiotics mixture (Antimycotic-A002A ?…”

Section: Cell Line Preparationmentioning

confidence: 99%

“…A few primary cultures of crayfish and prawn tissues have been developed earlier for the diagnosis of viruses infecting crustaceans (Uma et al 2002;Jiravanichpaisal et al 2006;Li and Shields 2007;Seena et al 2010) but long surviving and proliferating cell cultures were not available for evaluating viral infections. Since we reported long surviving proliferating testicular cell culture (cell line) of crab, Scylla serrata (Shashikumar and Desai 2011), it was decided to test our cell line of S. serrata (Forskal) as an in vitro system for studying pathogenesis of WSSV infection which is also known to infect crabs (Supamattaya et al1998;Liu et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Susceptibility of testicular cell cultures of crab, Scylla serrata (Forskal) to white spot syndrome virus

Shashikumar

Desai

2012

Cytotechnology

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Testicular cell culture of crab, Scylla serrata (Forskal) was used to study the effects of White spot syndrome virus (WSSV). We are showing the susceptibility of cell culture of crabs to WSSV. The proliferating cell culture of testes were maintained for more than 4 months in a medium prepared from L15 and crab saline supplemented with epidermal growth factor. The cell cultures inoculated with different concentrations of virus showed distinct cytopathic effects such as change in cell appearance, shrinkage and cell lysis. WSSV infection of cultured cells was confirmed by Nested PCR technique. The incorporation of viral DNA in cultured cells was shown by RAPD profile generated using 10-mer primers. The controls that were not exposed to WSSV did not show cytopathic effects. This work shows the usefulness of proliferating testicular cell culture for studying WSSV infection using molecular tools. Thus, this report gains significance as it opens new vistas for diagnostics and drugs for WSSV.

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“…The development of a cell line is essential for combatting any emerging viral disease (George and Dhar ). Primary cell cultures have been successfully developed by many researchers, and some were eventually adapted to study the pathogenesis of WSSV in various crab species (Sashikumar and Desai ; Shashikumar and Desai , ; Deepika et al. ).…”

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confidence: 99%

The Development and Characterization of a Cell Culture System from Indian Mud Crabs Scylla serrata

et al. 2019

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Commercially available culture media and supplements were tested for their potential to produce primary cell cultures from tissues of Indian mud crabs Scylla serrata. Eight commercially available culture media from Sigma-Aldrich (Leibovitz's L-15, Medium 199, Grace's Insect Medium, Minimal Essential Medium, Dulbecco's Modified Eagle Medium, TC-100 Insect Medium, IPL-41 Insect Medium, and Roswell Park Memorial Institute) were examined. Three different supplements (amino acid and sugar [AS], crab muscle extract [CME], and natural seawater [NSW]) were also examined. The hemocyte culture appeared to grow well for a maximum period of 21 d in 2 × L-15 medium supplemented with AS and 15% fetal bovine serum (FBS). Partial amplification and sequencing of the cytochrome oxidase subunit I (COI) gene confirmed that the primary hemocytes originated from Indian mud crabs. The effects of four metals on hemocyte viability were evaluated using the MTT assay. Of the four metals examined (arsenic, lead, cobalt, and nickel), cobalt and nickel were more toxic to the crab cells than the other metals. Both acridine orange/ethidium bromide and Hoechst staining showed the presence of apoptosis and necrosis in metal-treated groups, which suggests that metals in an aquatic environment induce death of the Indian mud crab's hemocytes. The hemocyte primary cell culture was also used to study the cytotoxicity effect of bacterial extracellular products from Vibrio harveyi and white spot syndrome virus. This study demonstrates that hemocyte primary cell culture can be used as a tool to study viral and bacterial pathogenesis and to assess the cytotoxicity of pollutants present in aquatic environments.

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Development of cell line from the testicular tissues of crab Scylla serrata

Cited by 16 publications

References 20 publications

Induction of vitellogenesis, MF synthesis and ecdysteroidogenesis in two edible crabs by arachidonic acid and prostaglandins

Induction of vitellogenesis, MF synthesis and ecdysteroidogenesis in two edible crabs by arachidonic acid and prostaglandins

Susceptibility of testicular cell cultures of crab, Scylla serrata (Forskal) to white spot syndrome virus

The Development and Characterization of a Cell Culture System from Indian Mud Crabs Scylla serrata

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