Development of capillary zone electrophoresis‐electrospray ionization‐mass spectrometry for the determination of lamotrigine in human plasma

Zheng, Jack; Jann, Michael W.; Hon, Yuen Yi; Shamsi, Shahab A.

doi:10.1002/elps.200305823

Cited by 55 publications

(42 citation statements)

References 33 publications

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“…Lastly, there is a fairly clear concentration threshold above which toxic side effects become more common [90,91]. Multiple analytical methodologies have been reported for the measurement of lamotrigine in plasma/serum including HPLC [92,93,94], radioimmunoassay [95], homogeneous immunoassay [96], immunofluorometric assay [97], capillary electrophoresis [98], capillary zone electrophoresis-electrospray ionization-mass spectrometry [99], gas chromatography (GC) with a nitrogen-phosphorus detector [100], GC/MS [101], LC/MS [102], LC/MS/MS [103] and micellar electrokinetic capillary chromatography [104]. …”

Section: Lamotriginementioning

confidence: 99%

Therapeutic Drug Monitoring of the Newer Anti-Epilepsy Medications

Krasowski

2010

Pharmaceuticals

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In the past twenty years, 14 new antiepileptic drugs have been approved for use in the United States and/or Europe. These drugs are eslicarbazepine acetate, felbamate, gabapentin, lacosamide, lamotrigine, levetiracetam, oxcarbazepine, pregabalin, rufinamide, stiripentol, tiagabine, topiramate, vigabatrin and zonisamide. In general, the clinical utility of therapeutic drug monitoring has not been established in clinical trials for these new anticonvulsants, and clear guidelines for drug monitoring have yet to be defined. The antiepileptic drugs with the strongest justifications for drug monitoring are lamotrigine, oxcarbazepine, stiripentol, and zonisamide. Stiripentol and tiagabine are strongly protein bound and are candidates for free drug monitoring. Therapeutic drug monitoring has lower utility for gabapentin, pregabalin, and vigabatrin. Measurement of salivary drug concentrations has potential utility for therapeutic drug monitoring of lamotrigine, levetiracetam, and topiramate. Therapeutic drug monitoring of the new antiepileptic drugs will be discussed in managing patients with epilepsy.

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Section: Lamotriginementioning

confidence: 99%

Therapeutic Drug Monitoring of the Newer Anti-Epilepsy Medications

Krasowski

2010

Pharmaceuticals

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“…These include radioimmunoassay, [3] high perfor-mance liquid chromatography (HPLC) methods with ultra-violet detection, [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] capillary electrophoresis, [22] gas chromatography-mass spectrometry (GC-MS), [23] electro-spray ionization-mass spectrometry (ESI-MS). [24,25] These conventional quantitative HPLC methods with classical ultra-violet or capillary electrophoresis detection provide insufficient sensitivity and longer chromatographic run time.…”

Section: Introduction Fig 1: Chemical Structure Of Lamotriginementioning

confidence: 99%

Development and Validation of Analytical Method for the Estimation of Lamotrigine in Human Plasma

Kumar¹

2017

WJPPS

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A Simple, rapid, selective and sensitive liquid chromatographic mass spectrometry method was developed and validated for the determination of Lamotrigine from human plasma. The drug was extracted by ethyl acetate. Lamotrigine was measured in plasma using a validated method with mass spectrometer detector. Chromatographic peaks were separated on 4μm Chromolith, RP C-18 (504.6mm, 4µ) using 80:20 v/v Phosphate buffer pH 2.5, Acetonitrile asmobile phase at a flow rate of 0.500 ml/min. The chromatograms showed good resolution and no interference from plasma. The mean recovery from human plasma was found to be above 85%. The method was linear over the concentration range of 5 μg/ml to 1200 μg/ml with coefficient of correlation (r2) 0.9987. Both intraday and interday accuracy and precision data showed good reproducibility. This method was successfully applied to pharmacokinetics studies.

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“…Over the years, CE coupled to electrospray ionization-mass spectrometry (ESI-MS) has been utilized as a bioanalytical tool for the analysis of drug compounds in biological samples [24][25][26][27][28][29]. Even though the most common detector in CE is the UV detector due to its easy manageability and low cost, it has the drawback of low sensitivity due to the short optical path length.…”

Section: Determination Of Pharmaceutical Compounds In Biological Samplesmentioning

confidence: 99%

“…I n r e c e n t y e a r s , a l a r g e n u m b e r o f p u b l i c ations have been provided on the general developments and biological applications of CE-ESI-MS [24][25][26][27][28][29]. Zheng et al developed a CZE-ESI-MS method for monitoring the antiepileptic drug lamotrigine in human plasma [27].…”

Section: Determination Of Pharmaceutical Compounds In Biological Samplesmentioning

confidence: 99%

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Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

Kapnissi‐Christodoulou¹

2012

Electrophoresis

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In addition, hydrogen bonding, dipole-dipole, and dispersive interactions can contribute to the solute partitioning between the two phases [4][5][6].CE was originally considered as a powerful analytical tool for the analysis of biological macromolecules. It has though, over the years, been extensively used for the separation of other compounds, such as chiral drugs, food additives, pesticides, inorganic ions, organic acids, and others. In this chapter, the ability of CE, and particularly CZE and MEKC, to be used for the qualitative and quantitative determination of compounds in pharmaceutical, biological and natural samples is investigated. In each approach, a number of studies are reported and discussed. These studies involve establishment of optimum separation conditions, method validation, optimization of sample-preparation procedure and application for the determination of the analytes under study in real samples. The first part of this chapter involves the determination of polyphenolic compounds using CZE with UVVis detector in red and white wines, while the second part involves the determination of pharmaceutical compounds in biological samples, such as blood and urine, using the hyphenated technique CE-MS (mass spectrometry). The third and final part emphasizes the importance of MEKC in chiral analysis since it has been known that usually only one enantiomer is active, while the other may be less active, inactive or has adverse effects. Determination of polyphenolic compounds in natural samplesPolyphenolic compounds exist in a variety of natural products, such as fruits, vegetables, beverages (tea, wine and juices), honey, cacao and herbs. They attract a lot of interest due to their beneficial implication in human health. They have been widely studied due to their antioxidant capacity and their association with several pathological conditions, such as hypertension, cardiovascular disease, dementia, and even cancer [7][8][9]. Therefore, due to their health significance, numerous analytical methods have, over the last decades, been developed for their separation, identification and quantitation in natural products [10][11][12][13].According to literature, the simplest CE method, CZE, proved to be the best method for the determination of polyphenolic compounds in wine samples [14][15][16][17]. In such studies, and in each case, when the optimum CZE method was applied to different red and white wines, it was established that red wines have higher levels of polyphenolic compounds than white wines and that the polyphenolic composition varies among different wines.

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Development of capillary zone electrophoresis‐electrospray ionization‐mass spectrometry for the determination of lamotrigine in human plasma

Cited by 55 publications

References 33 publications

Therapeutic Drug Monitoring of the Newer Anti-Epilepsy Medications

Therapeutic Drug Monitoring of the Newer Anti-Epilepsy Medications

Development and Validation of Analytical Method for the Estimation of Lamotrigine in Human Plasma

Method Development by Use of Capillary Electrophoresis and Applications in Pharmaceutical, Biological and Natural Samples

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