Development of camelina enhanced with drought stress resistance and seed oil production by co-overexpression of MYB96A and DGAT1C

Kim, Ryeo Jin; Kim, Hyun Uk; Suh, Mi Chung

doi:10.1016/j.indcrop.2019.111475

Cited by 20 publications

(8 citation statements)

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“…Seed phenotype analysis revealed that transgenic lines accumulated more oil and carbohydrate, but less protein than the control lines (Table 1 and 2). To date, the attempts to increase seed oil content in plants were mostly focused on altering the expression level of the genes and/or transcription factors involved in the oil biosynthetic pathway (Zou et al, 1997;Jako et al, 2001;Vigeolas et al, 2007;Kim et al, 2014;Van Erp et al, 2014, Kim et al, 2019Guo et al, 2020). It has been shown that the fatty acid biosynthesis pathway is highly dependent upon NADH and NADPH supplies (Geer et al, 1979;Slabas and Fawcett, 1992).…”

Section: Discussionmentioning

confidence: 99%

Increasing seed thiamin content impacts stored carbon partitioning and subsequent seedling stress tolerance

Davis

Harper

Shintani

2021

Preprint

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Thiamin and thiamin pyrophosphate (TPP) are essential components for the function of enzymes involved in the metabolism of carbohydrates and amino acids in living organisms. In addition to its role as a cofactor, thiamin plays a key role in resistance against biotic and abiotic stresses in plants. Most of the studies used exogenous thiamin to enhance stress tolerance in plants. In this study, we achieved this objective by genetically engineering Arabidopsis thaliana and Camelina sativa for the seed-specific co-overexpression of the Arabidopsis thiamin biosynthetic genes Thi4, ThiC, and ThiE. Elevated thiamin content in the seeds of transgenic plants was accompanied by the enhanced expression levels of transcripts encoding thiamin cofactor-dependent enzymes. Furthermore, seed germination and root growth in thiamin over-producing lines were more tolerant to oxidative stress caused by salt and paraquat treatments. The transgenic seeds also accumulated more oil (up to16.4% in Arabidopsis and17.9% in C. sativa) and carbohydrate but less protein than the control seeds. The same results were also observed in TPP over-producing Arabidopsis plants generated by the seed-specific overexpression of TPK1. Together, our findings suggest that thiamin and TPP over-production in transgenic lines confer a boosted abiotic stress tolerance and alter the seed carbon partitioning as well.

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Section: Discussionmentioning

confidence: 99%

Increasing seed thiamin content impacts stored carbon partitioning and subsequent seedling stress tolerance

Davis

Harper

Shintani

2021

Preprint

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“…Abiotic stresses such as heat, salinity, drought, and waterlogging are critical limiting factors that affect growth, development, seed yield, and quality in oilseed crops (Boem et al, 1996 ; Purty et al, 2008 ; Elferjani and Soolanayakanahally, 2018 ). To date, several mechanisms have been discovered to analyze the mechanism of heat stress tolerance, including overexpression of various miRNAs (Arshad et al, 2017 ), antioxidant enzymes (Saxena et al, 2020 ), as well as genes encoding many transcription factors (Hao et al, 2011 ; Zhu et al, 2018 ), proteins involved in antioxidant activities (Kim et al, 2019 ) or osmoprotectants, and proteins facilitating phytohormonal signaling pathways (Sahni et al, 2016 ) in oilseeds. The success of conventional plant breeding techniques has been extensively studied to regulate heat stress tolerance mechanisms in various crops including oilseeds, but these techniques are very time consuming and cumbersome.…”

Section: Mechanism Of Heat Stress Tolerance In Plantsmentioning

confidence: 99%

“…In the coming decades, the growing demand for oilseeds can be achieved by using advanced molecular breeding techniques such as complementary breeding tools, which would be very useful to accelerate all crop improvement programs to produce climate-resilient crops. While transgenic approaches have so far been successfully used in oilseeds to improve a wide range of traits (Meesapyodsuk et al, 2018 ; Na et al, 2018 ; Shah et al, 2018 ; Kim et al, 2019 ; Wang et al, 2019 ), only a small number of these devices have made it to the market due to poor public perception as well as the disproportionately high cost and length of existing regulatory processes (Mall et al, 2018 ). Therefore, in this review, we aim to analyze recent results on the response and tolerance to heat stress at the cell, organelle, and whole plant level and describe the numerous approaches used to increase heat tolerance in oilseed crops.…”

Section: Introductionmentioning

confidence: 99%

Adaptation Strategies to Improve the Resistance of Oilseed Crops to Heat Stress Under a Changing Climate: An Overview

et al. 2021

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Temperature is one of the decisive environmental factors that is projected to increase by 1. 5°C over the next two decades due to climate change that may affect various agronomic characteristics, such as biomass production, phenology and physiology, and yield-contributing traits in oilseed crops. Oilseed crops such as soybean, sunflower, canola, peanut, cottonseed, coconut, palm oil, sesame, safflower, olive etc., are widely grown. Specific importance is the vulnerability of oil synthesis in these crops against the rise in climatic temperature, threatening the stability of yield and quality. The natural defense system in these crops cannot withstand the harmful impacts of heat stress, thus causing a considerable loss in seed and oil yield. Therefore, a proper understanding of underlying mechanisms of genotype-environment interactions that could affect oil synthesis pathways is a prime requirement in developing stable cultivars. Heat stress tolerance is a complex quantitative trait controlled by many genes and is challenging to study and characterize. However, heat tolerance studies to date have pointed to several sophisticated mechanisms to deal with the stress of high temperatures, including hormonal signaling pathways for sensing heat stimuli and acquiring tolerance to heat stress, maintaining membrane integrity, production of heat shock proteins (HSPs), removal of reactive oxygen species (ROS), assembly of antioxidants, accumulation of compatible solutes, modified gene expression to enable changes, intelligent agricultural technologies, and several other agronomic techniques for thriving and surviving. Manipulation of multiple genes responsible for thermo-tolerance and exploring their high expressions greatly impacts their potential application using CRISPR/Cas genome editing and OMICS technology. This review highlights the latest outcomes on the response and tolerance to heat stress at the cellular, organelle, and whole plant levels describing numerous approaches applied to enhance thermos-tolerance in oilseed crops. We are attempting to critically analyze the scattered existing approaches to temperature tolerance used in oilseeds as a whole, work toward extending studies into the field, and provide researchers and related parties with useful information to streamline their breeding programs so that they can seek new avenues and develop guidelines that will greatly enhance ongoing efforts to establish heat stress tolerance in oilseeds.

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“…Research findings have reported the improvement of oil content in C. sativa via genetic engineering. For instance, overexpression of DGAT1 could increase the seed oil by 24% [31]; when being co-expressed with GPD1, the DGAT1 oil increase was up to 13% [32], and co-expression with MYB96A resulted in an increase of 21% in fatty acid levels [33]. Other genes could also enhance the seed oil content in C. sativa, such as AGG3 [34], HMA3 (under heavy metal stress) [35], WRI1 [36], the patatin-related phospholipase AIIId [37], FAX1 and ABCA9 [38] and ZmLEC1 [39].…”

Section: Introductionmentioning

confidence: 99%

In Silico Analysis of Fatty Acid Desaturases Structures in Camelina sativa, and Functional Evaluation of Csafad7 and Csafad8 on Seed Oil Formation and Seed Morphology

Raboanatahiry

Yin

Chen

et al. 2021

IJMS

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Fatty acid desaturases add a second bond into a single bond of carbon atoms in fatty acid chains, resulting in an unsaturated bond between the two carbons. They are classified into soluble and membrane-bound desaturases, according to their structure, subcellular location, and function. The orthologous genes in Camelina sativa were identified and analyzed, and a total of 62 desaturase genes were identified. It was revealed that they had the common fatty acid desaturase domain, which has evolved separately, and the proteins of the same family also originated from the same ancestry. A mix of conserved, gained, or lost intron structure was obvious. Besides, conserved histidine motifs were found in each family, and transmembrane domains were exclusively revealed in the membrane-bound desaturases. The expression profile analysis of C. sativa desaturases revealed an increase in young leaves, seeds, and flowers. C. sativa ω3-fatty acid desaturases CsaFAD7 and CsaDAF8 were cloned and the subcellular localization analysis showed their location in the chloroplast. They were transferred into Arabidopsis thaliana to obtain transgenic lines. It was revealed that the ω3-fatty acid desaturase could increase the C18:3 level at the expense of C18:2, but decreases in oil content and seed weight, and wrinkled phenotypes were observed in transgenic CsaFAD7 lines, while no significant change was observed in transgenic CsaFAD8 lines in comparison to the wild-type. These findings gave insights into the characteristics of desaturase genes, which could provide an excellent basis for further investigation for C. sativa improvement, and overexpression of ω3-fatty acid desaturases in seeds could be useful in genetic engineering strategies, which are aimed at modifying the fatty acid composition of seed oil.

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Development of camelina enhanced with drought stress resistance and seed oil production by co-overexpression of MYB96A and DGAT1C

Cited by 20 publications

References 75 publications

Increasing seed thiamin content impacts stored carbon partitioning and subsequent seedling stress tolerance

Increasing seed thiamin content impacts stored carbon partitioning and subsequent seedling stress tolerance

Adaptation Strategies to Improve the Resistance of Oilseed Crops to Heat Stress Under a Changing Climate: An Overview

In Silico Analysis of Fatty Acid Desaturases Structures in Camelina sativa, and Functional Evaluation of Csafad7 and Csafad8 on Seed Oil Formation and Seed Morphology

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