Development of calretinin immunoreactivity in the brainstem auditory nuclei of the barn owl (Tyto alba)

Kubke, M. Fabiana; Gauger, Bärbel; Basu, Lopa; Wagner, Hermann; Carr, Catherine

doi:10.1002/(sici)1096-9861(19991213)415:2<189::aid-cne4>3.0.co;2-e

Cited by 45 publications

(38 citation statements)

References 100 publications

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“…In P8 animals, in contrast, there was no gradient in CR+cell density dorsoventrally and a much weaker gradient in terms of %CR+. This is consistent with previous work that showed that with maturity, the CR+expression gradient disappears in both chick and barn owl (Kubke et al 1999;Parks et al 1997). However, even in P8 animals and in the dorsal-most sections, the percentage of CR + neurons was modest (56 %).…”

Section: Distribution Of Cr+neurons In Na Along the Tonotopic Axissupporting

confidence: 93%

“…Calretinin is an EFhand calcium-binding protein whose distribution and localization have been well characterized. The rabbitderived polyclonal antibody has been used extensively to characterize the auditory pathways in mammalian and several avian species including chicken (Rogers 1989;Kubke et al 1999;Parks et al 1997;Fredrich et al 2009). The mouse monoclonal antibody was obtained by immunizing mice with recombinant human calretinin-22 k and its specificity verified by immunoblot in chickens according to the product information sheet provided by Swant.…”

Section: Calretinin Antibody Specificitymentioning

confidence: 94%

“…Calretinin immunoreactivity was found in the neuropil, presumably in the axon terminals of the eighth nerve, but was also localized to the cytoplasm of cell bodies in NA and NM (Kubke et al 1999;Parks et al 1997). Over development, progressive CR expression coincides with the tonotopic axes in NA, NM, and NL.…”

Section: Introductionmentioning

confidence: 97%

“…The auditory brain stems of birds and mammals have been characterized by high expression levels of three calcium-binding proteins (CaBP): calretinin, calbindin, and parvalbumin (Braun 1990;Braun et al 1985Braun et al , 1991bCelio and Heizmann 1981;Friauf 1994;Kubke et al 1999;Lohmann and Friauf 1996;Parks et al 1997). The calcium buffering capability of these CaBPs may play a neuroprotective role against high activity levels or degeneration during hearing loss (Camp and Wijesinghe 2009;D'Orlando et al 2002;Idrizbegovic et al 2006;Parks et al 1997).…”

Section: Introductionmentioning

confidence: 99%

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Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Bloom

Williams

MacLeod

2014

JARO

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Multiple calcium-binding proteins (CaBPs) are expressed at high levels and in complementary patterns in the auditory pathways of birds, mammals, and other vertebrates, but whether specific members of the CaBP family can be used to identify neuronal subpopulations is unclear. We used double immunofluorescence labeling of calretinin (CR) in combination with neuronal markers to investigate the distribution of CR-expressing neurons in brainstem sections of the cochlear nucleus in the chicken (Gallus gallus domesticus). While CR was homogeneously expressed in cochlear nucleus magnocellularis, CR expression was highly heterogeneous in cochlear nucleus angularis (NA), a nucleus with diverse cell types analogous in function to neurons in the mammalian ventral cochlear nucleus. To quantify the distribution of CR in the total NA cell population, we used antibodies against neuronal nuclear protein (NeuN), a postmitotic neuronspecific nuclear marker. In NA neurons, NeuN label was variably localized to the cell nucleus and the cytoplasm, and the intensity of NeuN immunoreactivity was inversely correlated with the intensity of CR immunoreactivity. The percentage of CR+neurons in NA increased from 31 % in embryonic (E)17/18 chicks, to 44 % around hatching (E21), to 51 % in postnatal day (P) 8 chicks. By P8, the distribution of CR + neurons was uniform, both rostrocaudal and in the tonotopic (dorsoventral) axis.Immunoreactivity for the voltage-gated potassium ion channel Kv1.1, used as a marker for physiological type, showed broad and heterogeneous postsynaptic expression in NA, but did not correlate with CR expression. These results suggest that CR may define a subpopulation of neurons within nucleus angularis.

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Section: Distribution Of Cr+neurons In Na Along the Tonotopic Axissupporting

confidence: 93%

Section: Calretinin Antibody Specificitymentioning

confidence: 94%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Bloom

Williams

MacLeod

2014

JARO

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“…They label neurons in the central auditory system of mammals such as rat (Lohmann and Friauf, 1996;Pór et al, 2005), guinea pig (Caicedo et al, 1996), and human (Bazwinsky et al, 2003); in birds such as chicken (Parks et al, 1997) and barn owl (Kubke et al, 1999;Takahashi et al, 1987); and in lizards (Dávila et al, 2000), turtles (Belekhova et al, 2004), and amphibians (Morona and González, 2009). We therefore used antibodies against calretinin (CR), parvalbumin (PV), and calbindin-D28k (CB), along with antibodies against glutamic acid decarboxylase (GAD) and synaptic vesicle protein 2 (SV2), to describe the gecko ascending auditory pathways.…”

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confidence: 99%

Calcium‐binding protein immunoreactivity characterizes the auditory system of Gekko gecko

Yang

Tang

Carr

2010

J of Comparative Neurology

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Geckos use vocalizations for intraspecific communication, but little is known about the organization of their central auditory system. We therefore used antibodies against the calciumbinding proteins calretinin (CR), parvalbumin (PV), and calbindin-D28k (CB) to characterize the gecko auditory system. We also examined expression of both glutamic acid decarboxlase (GAD) and synaptic vesicle protein (SV2). Western blots showed that these antibodies are specific to gecko brain. All three calcium-binding proteins were expressed in the auditory nerve, and CR immunoreactivity labeled the first-order nuclei and delineated the terminal fields associated with the ascending projections from the first-order auditory nuclei. PV expression characterized the superior olivary nuclei, whereas GAD immunoreactivity characterized many neurons in the nucleus of the lateral lemniscus and some neurons in the torus semicircularis. In the auditory midbrain, the distribution of CR, PV, and CB characterized divisions within the central nucleus of the torus semicircularis. All three calcium-binding proteins were expressed in nucleus medialis of the thalamus. These expression patterns are similar to those described for other vertebrates.Keywords cochlear nucleus; magnocellularis; laminaris; angularis; torus Over 30 years have passed since Foster and Hall (1978) reported that the ascending auditory pathways of the iguana resemble both the mammalian and the avian auditory pathways, from the level of the first-order neurons in the VIIIth nerve to the telencephalon. They recognized a basic plan for the organization of the auditory system in terrestrial vertebrates but also pointed out that the auditory capacities of the lizards were largely unknown. We now know more about the peripheral (Manley, 1990;Wever, 1978) and central (for reviews see Carr, 1992;Grothe et al., 2005;ten Donkelaar et al., 1987) auditory systems of lizards, but still know little about central auditory processing. We have therefore used Gekko gecko for further analysis of the lizard central auditory system.Geckos are auditory specialists that use vocalizations for intraspecific communication (Marcellini, 1977;Tang et al., 2001). Their ears differ from those of other reptiles such as archosaurs and turtles. Lepidosaur ears are highly directional, with middle ears connected through the mouth cavity (Christensen-Dalsgaard and Manley, 2008). This patent connection enhances the directionality of the ear by allowing sound access to both sides of NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript each tympanic membrane. The acoustically coupled ear creates directional responses from the tympanum Manley, 2005, 2008). Additionally, lizards have independently evolved micromechanical hair cell tuning, permitting emergence of sensitive high-frequency hearing in a specialized region of the papilla (Manley, 2002). Thus lizard auditory systems might reveal specializations for hearing high frequencies and sound localization.We have begun our analysis of liza...

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The cytoarchitecture of the nucleus angularis of the barn owl (Tyto alba)

Soares

Carr

2000

J. Comp. Neurol.

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The cochlear nucleus angularis (NA) of the barn owl (Tyto alba) was analyzed using Golgi, Nissl, and tract tracing techniques. NA forms a column of cells in the dorsolateral brainstem that partly overlaps with, and is rostral and lateral to, the cochlear nucleus magnocellularis (NM). Highest best frequencies are mapped in lateral NA (NAl), intermediate in medial NA (NAm), and lowest in the foot region (NAf). Cell density followed the tonotopic axis and decreased with decreasing best frequency. NA contained four major cell classes: planar, radiate, vertical, and stubby. Planar and radiate classes were further subdivided into bipolar and multipolar types according to their number of primary dendrites. Planar neurons were confined to an isofrequency band, whereas radiate neurons had dendrites that could extend across an isofrequency band. Vertical cells had long dendrites oriented perpendicularly to isofrequency bands. Stubby cells were the most numerous and were confined to an isofrequency band because of their short dendrites. Neurons in each of these four classes projected to the inferior colliculus and dorsal nucleus of the lateral lemniscus.

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Development of calretinin immunoreactivity in the brainstem auditory nuclei of the barn owl (Tyto alba)

Cited by 45 publications

References 100 publications

Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Heterogeneous Calretinin Expression in the Avian Cochlear Nucleus Angularis

Calcium‐binding protein immunoreactivity characterizes the auditory system of Gekko gecko

The cytoarchitecture of the nucleus angularis of the barn owl (Tyto alba)

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