Development of Calbindin- and Calretinin-Immunopositive Neurons in the Enteric Ganglia of Rats

Maslyukov, P. M.; Moiseev, K. Yu.; Budnik, A. F.; Nozdrachev, A. D.; Timmermans, Jean‐Pierre

doi:10.1007/s10571-016-0457-x

Cited by 15 publications

(7 citation statements)

References 60 publications

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“…In the SP and MP, the average cross-sectional areas of nNOS (+) and nNOS (À) neurons did not significantly differ from each other. Literature data indicate that in early postnatal ontogenesis there is an increase in the size of neurons in the ganglia of the autonomic nervous system, including sympathetic and enteric (Masliukov et al, 2016(Masliukov et al, , 2017.…”

Section: Discussionmentioning

confidence: 99%

Postnatal development of the enteric neurons expressing neuronal nitric oxide synthase

Budnik

Maslyukov

2022

The Anatomical Record

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Neurons, expressing neuronal nitric oxide synthase (nNOS) in the enteric ganglia are inhibitory motor neurons or interneurons. The aim of the study was to identify the percentage, cross‐sectional area of nNOS‐immunoreactive (IR) neurons and their colocalization with choline acetyltransferase (ChAT), vasoactive intestinal polypeptide (VIP), and neuropeptide Y in the intramural ganglia of the myenteric (MP) and submucous plexus (SP) of the small intestine (SI) and large intestine (LI) of rats of different age groups using immunohistochemical methods. In the intramural ganglia of the MP, the largest percentage of nNOS‐IR neurons was detected in newborn rats in the LI (81 ± 0.9%) and SI (48 ± 4.1%). Subsequently, it decreased in ontogenesis up to 60 days of life (26 ± 0.9% LI, 29 ± 3.2% SI), and did not change until senescence. In the SP, abundant nNOS‐IR neurons were also detected in newborns (82 ± 7.0% SI, 85 ± 3.2% LI), while their percentage decreased significantly in the next 20 days. Furthermore, a very small number of nNOS‐IR neurons was detected in 30‐day‐ and 2‐month‐old animals, but they again appeared in large numbers in aged rats. In the MP, the highest percentage of nNOS+/ChAT+ neurons was in 1‐day‐old, 10‐day‐old, and 2‐year‐old rats. In the SP, the largest number of nNOS‐IR neurons colocalized ChAT regardless of age. In the MP of all rats, many nNOS‐IR neurons colocalized VIP, and the maximal percentage of nNOS+/VIP+ neurons was found in 2‐year‐old rats, minimal—in newborns. In conclusion, nNOS expression in neurons of the gut is decreased in early postnatal ontogenesis and subsequently increased in aged rats.

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Section: Discussionmentioning

confidence: 99%

Postnatal development of the enteric neurons expressing neuronal nitric oxide synthase

Budnik

Maslyukov

2022

The Anatomical Record

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“…Different subpopulations of neurons expressed these two calcium‐binding proteins in the rat hippocampus (Miettinen, Gulyás, Baimbridge, Jacobowitz, & Freund, 1992) and hypothalamic arcuate nucleus (Foo et al, 2014). In the rat enteric nervous system, the majority of the IR neurons colocalized CR and CB in young and aged rats (Masliukov et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…For the antibody specificity, the controls were preabsorption with the primary antibody to the CB‐ and CR‐immunogenic peptide (Abcam, ab82631 and ab100941) or the replaicement of the primary antibody with normal rabbit serum. For positive control we used the small intestine, where many enteric neurons were immunopositive to CB and CR in young and aged rats (Masliukov, Moiseev, Budnik, Nozdrachev, & Timmermans, 2017).…”

Section: Methodsmentioning

confidence: 99%

Expression of calbindin and calretinin in the dorsomedial and ventromedial hypothalamic nuclei during aging

Vishnyakova

Moiseev

Spirichev

et al. 2020

The Anatomical Record

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The hypothalamus is involved in the regulation of rhythms, autonomic, endocrine, and behavioral functions and may also participate in aging development and control. The aim of this work was to study the expression of calbindin (CB) and calretinin (CR) in the ventromedial (VMH) and dorsomedial (DMH) hypothalamic nuclei in young and old rats of both sexes by immunohistochemistry and western blotting. In young animals, the largest number of CB‐immunoreactive (IR) neurons was detected in the ventral part of DMH (DMHv) and smaller percentage was found in its dorsal part (DMHd), in the dorsomedial part of the VMH (VMHdm) and in the ventrolateral part of the VMH (VMHvl). In aged animals, the percentage of CB‐IR neurons significantly decreased in all studied nuclei, including DMHv, DMHd, VMHdm and VMHvl. CR‐IR neurons were found in moderate number in the DMHv, DMHd, VMHdm and VMHvl of young rats. In aged rats, the percentage of CR‐IR neurons significantly increased in the DMHv, DMHd, VMHdm and VMHvl. Less than one third of IR neurons colocalized CB and CR in young and aged rats. The expression of CB significantly decreased, and the expression of CR significantly increased in the DMH and VMH during aging by western blot analysis. Thus, there are opposite changes of the calcium‐binding proteins expression in the hypothalamic nuclei involved in the metabolic and sexual regulation during aging.

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“…9 Calretinin immunohistochemistry has become a routine ancillary method in the histopathological diagnosis of Hirschsprung disease. 10 Expression of calretinin in a subset of enteric neurons is a conserved feature in other animals (e.g., mice, rats, sheep, and guinea pig), 11 but its function is unknown and mice that lack calretinin have no obvious gastrointestinal phenotype. 12 To date, calretinin expression has not been reported in skeletal, cardiac, or smooth muscle cells.…”

Section: Introductionmentioning

confidence: 99%

Expression of Calretinin in the Cecal Muscularis Interna: Observation and Hypothetical Relevance to Appendicitis

Kapur

2024

Pediatr Dev Pathol

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Background: The unexpected observation of calretinin immunoreactivity in smooth muscle cells in the muscularis propria of the cecum led to a more detailed examination of calretinin expression and its possible relationship to propulsive contractile activity around the vermiform appendix. Methods: Immunohistochemistry and RNA in situ hybridization were performed to analyze calretinin expression in intestinal samples from 33 patients at ages ranging from mid-gestation fetuses to adults, as well as in some potentially relevant animal models. Dual immunolabeling was done to compare calretinin localization with markers of smooth muscle and interstitial cells of Cajal. Results: Calretinin expression was observed consistently in the innermost smooth muscle layers of the muscularis interna in the human cecum, appendiceal base, and proximal ascending colon, but not elsewhere in the intestinal tract. Calretinin-positive smooth muscle cells did not co-express markers located in adjacent interstitial cells of Cajal. Muscular calretinin immunoreactivity was not detected in the ceca of mice or macaques, species which lack appendices, nor in the rabbit cecum or appendix. Conclusions: Localized expression of calretinin in cecal smooth muscle cells may reduce the likelihood of retrograde, calcium-mediated propulsive contractions from the proximal colon and suppress pro-inflammatory fecal stasis in the appendix.

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Development of Calbindin- and Calretinin-Immunopositive Neurons in the Enteric Ganglia of Rats

Cited by 15 publications

References 60 publications

Postnatal development of the enteric neurons expressing neuronal nitric oxide synthase

Postnatal development of the enteric neurons expressing neuronal nitric oxide synthase

Expression of calbindin and calretinin in the dorsomedial and ventromedial hypothalamic nuclei during aging

Expression of Calretinin in the Cecal Muscularis Interna: Observation and Hypothetical Relevance to Appendicitis

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