Development of bone-like composites via the polymer-induced liquid-precursor (PILP) process. Part 1: Influence of polymer molecular weight

Jee, Sang-Soo; Thula, Taili T.; Gower, Laurie B.

doi:10.1016/j.actbio.2010.03.036

Cited by 152 publications

(175 citation statements)

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“…[32,117] The adjunction of acidic polymers (i.e., simple analogs of NCPs) to the supersaturated mineralization solution stabilizes a highly-hydrated amorphous precursor and simultaneously delays nucleation and growth of crystals to form a metastable solution. [32,[140][141][142][143] The collagen fibrils themselves can actively regulate the nucleation of oriented HA crystals. Recently, OPN, one of the NCPs in bone ECM, was applied in the PILP process, which is similarly served as a process-directing agent for the IMC formation.…”

Section: Polymer-induced Liquid-precursor (Pilp) Pathway For Intrafibmentioning

confidence: 99%

Hierarchical Structures of Bone and Bioinspired Bone Tissue Engineering

Liu

Luo

Wang

2016

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Bone, as a mineralized composite of inorganic (mostly carbonated hydroxyapatite) and organic (mainly type I collagen) phases, possesses a unique combination of remarkable strength and toughness. Its excellent mechanical properties are related to its hierarchical structures and precise organization of the inorganic and organic phases at the nanoscale: Nanometer-sized hydroxyapatite crystals periodically deposit within the gap zones of collagen fibrils during bone biomineralization process. This hierarchical arrangement produces nanomechanical heterogeneities, which enable a mechanism for high energy dissipation and resistance to fracture. The excellent mechanical properties integrated with the hierarchical nanostructure of bone have inspired chemists and material scientists to develop biomimetic strategies for artificial bone grafts in tissue engineering (TE). This critical review provides a broad overview of the current mechanisms involved in bone biomineralization, and the relationship between bone hierarchical structures and the deformation mechanism. Our goal in this review is to inspire the application of these principles toward bone TE.

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Section: Polymer-induced Liquid-precursor (Pilp) Pathway For Intrafibmentioning

confidence: 99%

Hierarchical Structures of Bone and Bioinspired Bone Tissue Engineering

Liu

Luo

Wang

2016

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314

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“…Subsequently, due to the transformation of ACP to HA, collagen fibrils are embedded with HA nanocrystals similar to the natural bone structure. 39,40 Based on this bionic concept, we have found a new type of analog called chitosan, which is derived from the shells of shellfish and natural polysaccharides of some fungal cell walls. It has been reported that carboxymethyl chitosan (CMC), the derivative of chitosan enriched in carboxyl groups, can retard or inhibit the rate of spontaneous calcium phosphate precipitation.…”

Section: Introductionmentioning

confidence: 99%

Synergistic intrafibrillar/extrafibrillar mineralization of collagen scaffolds based on a biomimetic strategy to promote the regeneration of bone defects

Wang¹,

Manh²,

Wang³

et al. 2016

IJN

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The mineralization of collagen scaffolds can improve their mechanical properties and biocompatibility, thereby providing an appropriate microenvironment for bone regeneration. The primary purpose of the present study is to fabricate a synergistically intra- and extrafibrillar mineralized collagen scaffold, which has many advantages in terms of biocompatibility, biomechanical properties, and further osteogenic potential. In this study, mineralized collagen scaffolds were fabricated using a traditional mineralization method (ie, immersed in simulated body fluid) as a control group and using a biomimetic method based on the polymer-induced liquid precursor process as an experimental group. In the polymer-induced liquid precursor process, a negatively charged polymer, carboxymethyl chitosan (CMC), was used to stabilize amorphous calcium phosphate (ACP) to form nanocomplexes of CMC/ACP. Collagen scaffolds mineralized based on the polymer-induced liquid precursor process were in gel form such that nanocomplexes of CMC/ACP can easily be drawn into the interstices of the collagen fibrils. Scanning electron microscopy and transmission electron microscopy were used to examine the porous micromorphology and synergistic mineralization pattern of the collagen scaffolds. Compared with simulated body fluid, nanocomplexes of CMC/ACP significantly increased the modulus of the collagen scaffolds. The results of in vitro experiments showed that the cell count and differentiated degrees in the experimental group were higher than those in the control group. Histological staining and micro-computed tomography showed that the amount of new bone regenerated in the experimental group was larger than that in the control group. The biomimetic mineralization will assist us in fabricating a novel collagen scaffold for clinical applications.

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“…For example, using a polymer-induced, liquid-precursor process, collagen/apatite biocomposites mimicking the nanostructure of bones, wherein nano-sized crystals of apatite were embedded within the collagen fibrils, were prepared. 757 More complicated formulations, such as magnetite-enriched HA/collagen 758 and HA/collagen/PVA 759 biocomposites, have been developed as well. Furthermore, collagen might be incorporated into various calcium orthophosphate cements.…”

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confidence: 99%