Development of Bionanocomposites Based on PLA, Collagen and AgNPs and Characterization of Their Stability and In Vitro Biocompatibility

Râpă, Maria; Stefan, Laura; Zaharescu, Traian; Seciu, Ana‐Maria; Țurcanu, Anca Andreea; Matei, Ecaterina; Predescu, Andra Mihaela; Antoniac, Iulian Vasile; Predescu, Cristian

doi:10.3390/app10072265

Cited by 17 publications

(9 citation statements)

References 57 publications

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“…Collagen is an expensive material obtained from bovine and swine hides, as well as fish skins [15,16] and usually used in its native state. Collagen hydrolysate obtained by the enzymatic extraction of bovine tendons was successfully used to increase the biocompatibility and degradability of poly(lactic acid) (PLA) [17][18][19]. The main disadvantage of these PLA biocomposites is the poor compatibility between collagen hydrolysate and synthetic polymeric matrix.…”

Section: Introductionmentioning

confidence: 99%

New Nanofibers based on Protein By-Products with Bioactive Potential for Tissue Engineering

Râpă¹,

Gaidău²,

Stefan³

et al. 2020

Prime Archives in Material Science

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Concentrated collagen hydrolysate (HC10CC), rabbit collagen glue (RCG) and keratin hydrolysate (KH) were investigated in terms of their extraction from mammalian by-products and processing by electrospinning. The electrospun nanofibers were characterized by scanning electron microscopy coupled with the energy dispersive X-ray spectroscopy (SEM/EDS), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FT-IR), differential scanning calorimetry (DSC), and indentation tests. The cytotoxicity of the electrospun nanofibers was conducted on L929 fibroblast cells using MTT and LDH assays and cell morphology observations. The electrospun RCG and KH nanofibers morphology showed an average size of nanofibers ranging between 44 and 410 nm, while the electrospun HC10CC nanofibers exhibited higher sizes. The ATR-FT-IR spectra performed both on extracted proteins and electrospun nanofibers showed that the triple helix structure of collagen is partially preserved. The results were in agreement with the circular dichroism analysis for protein extracts. Also, the viscoelastic properties of electrospun KH nanofibers were superior to those of electrospun RCG nanofibers. Based on both quantitative and qualitative analysis, the electrospun nanofibers were not cytotoxic, inducing a healthy cellular response. The results of new electrospun protein-based nanofibers may be useful for further research on bioactive properties of these nanofibers for tissue engineering.

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Section: Introductionmentioning

confidence: 99%

New Nanofibers based on Protein By-Products with Bioactive Potential for Tissue Engineering

Râpă¹,

Gaidău²,

Stefan³

et al. 2020

Prime Archives in Material Science

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“…The optical density directly corresponds to the number of metabolically active cells. The results were assessed as the percentage of viability compared to the control (untreated cells) considered 100% viable (Râpȃ et al, 2020). The sample is considered potentially cytotoxic if cell viability falls below 70% control viability (untreated cells) ISO 10993-5 (International Organization for Standardization [ISO]., 2009;Liu et al, 2018).…”

Section: Diphenyltetrazolium Bromide Viability Assaysmentioning

confidence: 99%

Biocompatibility and biocidal effects of modified polylactide composites

et al. 2022

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Polylactide (PLA) materials treated with antimicrobial fillers represent a suitable alternative to the production of medical devices. Their advantage is that they can prevent the growth of microorganisms and the formation of microbial biofilms on the surface and around composites. The work is focused on the evaluation of biocompatibility and biocide effect of PLA composite films filled with vermiculite and graphene oxide modified with silver (Ag+ and Ag nanoparticles), hexadecylpyridinium (HDP) and hexadecyltrimethylammonium (HDTMA) cations and their degradation leachates monitored at 1–3–6-month intervals. The antimicrobial effect of the leachates was detected by microdilution methods on gram-negative (Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis), gram-positive (Staphylococcus aureus, Streptococcus salivarius) bacteria and yeast (Candida albicans). The biocidal effect of composites on biofilm formation on the surface of composites was monitored by Christensen method and autoaggregation and motility tests. The biocompatibility of the composite and the leachates was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) cytotoxicity assay. The evaluation of the antimicrobial effect of the leachates demonstrated that leachates of PLA composite filled with graphene oxide and Ag+ showed a stronger antimicrobial effect than leachates of PLA composite filled with vermiculite and Ag+ and Ag nanoparticles. The leachates of PLA composites containing vermiculite with HDP and HDTMA cations had a higher antimicrobial effect on G+ bacteria and yeast than G– bacteria. Bacterial growth, biofilm formation, autoaggregation and motility of the tested bacteria were most inhibited by the composite with vermiculite and Ag+ and Ag nanoparticles. Even after a 6-month degradation of this composite, bacterial growth and biofilm formation continued to be strongly inhibited up to 42 and 91%, respectively. The cytotoxic effect was proved only in the leachate of the composite with vermiculite containing HDP after 6 months of its degradation. Tests evaluating the biocompatibility of materials have shown that the vermiculite is the most preferred carrier and can be used in the future to bind other compounds. The study confirmed that PLA composite filled with vermiculite and Ag+ and Ag nanoparticles was the most stable and effective composite with the best biocompatible and biocidal properties.

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“…Metallic and metallic oxide nanoparticles, including ZnO, copper, silver, gold, and platinum, are commonly known to possess bactericidal properties [64][65][66]. Among these inorganic antibacterial agents, silver ions and compounds were extensively investigated and studied [67][68][69][70]. Silver nanoparticles (AgNPs) possess the capability to interact with a diverse range of microorganisms, including mature bacterial biofilms.…”

Section: Introductionmentioning

confidence: 99%

Influence of Lavender Essential Oil on the Physical and Antibacterial Properties of Chitosan Sponge for Hemostatic Applications

Gheorghiță,

Antoniac,

Moldovan

et al. 2023

IJMS

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Uncontrollable bleeding continues to stand as the primary cause of fatalities globally following surgical procedures, traumatic incidents, disasters, and combat scenarios. The swift and efficient management of bleeding through the application of hemostatic agents has the potential to significantly reduce associated mortality rates. One significant drawback of currently available hemostatic products is their susceptibility to bacterial infections at the bleeding site. As this is a prevalent issue that can potentially delay or compromise the healing process, there is an urgent demand for hemostatic agents with antibacterial properties to enhance survival rates. To mitigate the risk of infection at the site of a lesion, we propose an alternative solution in the form of a chitosan-based sponge and antimicrobial agents such as silver nanoparticles (AgNPs) and lavender essential oil (LEO). The aim of this work is to provide a new type of hemostatic sponge with an antibacterial barrier against a wide range of Gram-positive and Gram-negative microorganisms: Staphylococcus epidermidis 2018 and Enterococcus faecalis VRE 2566 (Gram-positive strains) and Klebsiella pneumoniae ATCC 10031 and Escherichia coli ATCC 35218 (Gram-negative strains).

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Development of Bionanocomposites Based on PLA, Collagen and AgNPs and Characterization of Their Stability and In Vitro Biocompatibility

Cited by 17 publications

References 57 publications

New Nanofibers based on Protein By-Products with Bioactive Potential for Tissue Engineering

New Nanofibers based on Protein By-Products with Bioactive Potential for Tissue Engineering

Biocompatibility and biocidal effects of modified polylactide composites

Influence of Lavender Essential Oil on the Physical and Antibacterial Properties of Chitosan Sponge for Hemostatic Applications

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