Development of biofactory-on-a-chip technology using excimer laser micromachining

Pethig, Ronald; Burt, Julian P.H.; Parton, A; Rizvi, Nadeem Hasan; Talary, Mark S.; Tame, John A.

doi:10.1088/0960-1317/8/2/004

Cited by 89 publications

(50 citation statements)

References 11 publications

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“…Examples of this are glass-based chips, combining traveling-wave DEP and electrorotation, to concentrate and assay the viability of microorganisms, 101 and structures incorporating DEP, traveling-wave DEP, and electrorotation for characterizing and selectively trapping cells, microorganisms, and other particles. 102 Figure 11 shows a traveling-wave DEP junction, fabricated by excimer laser ablation and designed to bring together or separate different particle types. 102 Combining the complementary techniques of DEP, traveling-wave DEP, and electrorotation is a continuing trend.…”

Section: Technologymentioning

confidence: 99%

“…102 Figure 11 shows a traveling-wave DEP junction, fabricated by excimer laser ablation and designed to bring together or separate different particle types. 102 Combining the complementary techniques of DEP, traveling-wave DEP, and electrorotation is a continuing trend. For example, DEP and traveling-wave DEP have been combined onto a single, personal computer ͑PC͒-controlled, printed circuit board and tested by manipulating tumor cells.…”

Section: Technologymentioning

confidence: 99%

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Review Article—Dielectrophoresis: Status of the theory, technology, and applications

2010

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A review is presented of the present status of the theory, the developed technology and the current applications of dielectrophoresis ͑DEP͒. Over the past 10 years around 2000 publications have addressed these three aspects, and current trends suggest that the theory and technology have matured sufficiently for most effort to now be directed towards applying DEP to unmet needs in such areas as biosensors, cell therapeutics, drug discovery, medical diagnostics, microfluidics, nanoassembly, and particle filtration. The dipole approximation to describe the DEP force acting on a particle subjected to a nonuniform electric field has evolved to include multipole contributions, the perturbing effects arising from interactions with other cells and boundary surfaces, and the influence of electrical double-layer polarizations that must be considered for nanoparticles. Theoretical modelling of the electric field gradients generated by different electrode designs has also reached an advanced state. Advances in the technology include the development of sophisticated electrode designs, along with the introduction of new materials ͑e.g., silicone polymers, dry film resist͒ and methods for fabricating the electrodes and microfluidics of DEP devices ͑photo and electron beam lithography, laser ablation, thin film techniques, CMOS technology͒. Around three-quarters of the 300 or so scientific publications now being published each year on DEP are directed towards practical applications, and this is matched with an increasing number of patent applications. A summary of the US patents granted since January 2005 is given, along with an outline of the small number of perceived industrial applications ͑e.g., mineral separation, micropolishing, manipulation and dispensing of fluid droplets, manipulation and assembly of micro components͒. The technology has also advanced sufficiently for DEP to be used as a tool to manipulate nanoparticles ͑e.g., carbon nanotubes, nano wires, gold and metal oxide nanoparticles͒ for the fabrication of devices and sensors. Most efforts are now being directed towards biomedical applications, such as the spatial manipulation and selective separation/enrichment of target cells or bacteria, high-throughput molecular screening, biosensors, immunoassays, and the artificial engineering of three-dimensional cell constructs. DEP is able to manipulate and sort cells without the need for biochemical labels or other bioengineered tags, and without contact to any surfaces. This opens up potentially important applications of DEP as a tool to address an unmet need in stem cell research and therapy.

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Section: Technologymentioning

confidence: 99%

Section: Technologymentioning

confidence: 99%

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

2010

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“…19 The use of TWD as a general motive force led to the development of putative "biofactories on a chip," highly complicated devices where railway tracks of electrodes would peel away from one another, sending populations in different directions. 20 Ultimately, the use of TWD as a separation technology waned through the early 2000s as the limitations associated with the technology became apparent. Chief among these was the power requirements to overcome losses in the large number of electrodes required to move cells; the large scale electrode arrays would struggle to move cells along the required distances.…”

Section: The Microfabrication Explosionmentioning

confidence: 99%

Fifty years of dielectrophoretic cell separation technology

Hughes

2016

Biomicrofluidics

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In 1966, Pohl and Hawk [Science 152, 647–649 (1966)] published the first demonstration of dielectrophoresis of living and dead yeast cells; their paper described how the different ways in which the cells responded to an applied nonuniform electric field could form the basis of a cell separation method. Fifty years later, the field of dielectrophoretic (DEP) cell separation has expanded, with myriad demonstrations of its ability to sort cells on the basis of differences in electrical properties without the need for chemical labelling. As DEP separation enters its second half-century, new approaches are being found to move the technique from laboratory prototypes to functional commercial devices; to gain widespread acceptance beyond the DEP community, it will be necessary to develop ways of separating cells with throughputs, purities, and cell recovery comparable to gold-standard techniques in life sciences, such as fluorescence- and magnetically activated cell sorting. In this paper, the history of DEP separation is charted, from a description of the work leading up to the first paper, to the current dual approaches of electrode-based and electrodeless DEP separation, and the path to future acceptance outside the DEP mainstream is considered.

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“…Ranges of polymeric material have been used with this method, including PMMA, PS, PC (PET) [105,106]. Bonding of PMMA-based microstructured devices can be achieved by using solvent-assisted gluing, melting, laminating and surface activation.…”

Section: Laser Ablation Micromachiningmentioning

confidence: 99%

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

2012

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This paper reviews microfluidic technologies with emphasis on applications in the fields of pharmacy, biology, and tissue engineering. Design and fabrication of microfluidic systems are discussed with respect to specific biological concerns, such as biocompatibility and cell viability. Recent applications and developments on genetic analysis, cell culture, cell manipulation, biosensors, pathogen detection systems, diagnostic devices, high-throughput screening and biomaterial synthesis for tissue engineering are presented. The pros and cons of materials like polydimethylsiloxane (PDMS), polymethylmethacrylate (PMMA), polystyrene (PS), polycarbonate (PC), cyclic olefin copolymer (COC), glass, and silicon are discussed in terms of biocompatibility and fabrication aspects. Microfluidic devices are widely used in life sciences. Here, commercialization and research trends of microfluidics as new, easy to use, and cost-effective measurement tools at the cell/tissue level are critically reviewed.

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Development of biofactory-on-a-chip technology using excimer laser micromachining

Cited by 89 publications

References 11 publications

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

Fifty years of dielectrophoretic cell separation technology

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

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