Development of bioengineered human larynx

Baiguera, Silvia; Gonfiotti, Alessandro; Jaus, Massimo Osvaldo; Comin, Camilla E.; Paglierani, Milena; Gaudio, Costantino Del; Bianco, Alessandra; Ribatti, Doménico; Macchiarini, Paolo

doi:10.1016/j.biomaterials.2011.02.055

Cited by 93 publications

(77 citation statements)

References 38 publications

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“…SDC is a hypertonic detergent and may therefore be a suitable decellularization reagent. Furthermore, SDC has successfully been used for trachea, larynx and small intestine decellularization and has shown to retain some angiogenic factors in the remaining matrix [14,[35][36][37]. SDC is generally considered to be a less aggressive detergent than the commonly used SDS [29,30,38].…”

Section: Discussionmentioning

confidence: 98%

“…Maintaining an appropriate level on the immune system may be necessary [47], and even if one of the main objectives with tissue engineered organs is to overcome the need for immunosuppressive treatment after transplantation, clinicians may consider administering a shortterm immunosuppressive therapy while the last remaining immunogens from the scaffold are cleared and replaced with autologous cell/protein components. In order to establish important additional functional information, experiments to evaluate growth factor bioactivity and/or angiogenic properties of the scaffolds will be considered in the future [35][36][37].…”

Section: Discussionmentioning

confidence: 99%

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Towards the development of a bioengineered uterus: Comparison of different protocols for rat uterus decellularization

et al. 2014

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Towards the development of a bioengineered uterus: Comparison of different protocols for rat uterus decellularization

et al. 2014

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“…A lthough replacement of airways (1)(2)(3)(4)(5)(6) or urogenital tissue (7) using stem cell-based techniques has been achieved, engineering of functional contractile muscle tissue has only been partially explored (8)(9). While debates have been generated around which myogenic progenitors (satellite cells, muscle stem cells, or myoblasts) should be used, fewer studies have focused on exploring which matrix could offer a better platform for regeneration (10,11).…”

mentioning

confidence: 99%

Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

Fishman

Lowdell

Urbani³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

181

137

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Decellularized (acellular) scaffolds, composed of natural extracellular matrix, form the basis of an emerging generation of tissueengineered organ and tissue replacements capable of transforming healthcare. Prime requirements for allogeneic, or xenogeneic, decellularized scaffolds are biocompatibility and absence of rejection. The humoral immune response to decellularized scaffolds has been well documented, but there is a lack of data on the cellmediated immune response toward them in vitro and in vivo. Skeletal muscle scaffolds were decellularized, characterized in vitro, and xenotransplanted. The cellular immune response toward scaffolds was evaluated by immunohistochemistry and quantified stereologically. T-cell proliferation and cytokines, as assessed by flow cytometry using carboxy-fluorescein diacetate succinimidyl ester dye and cytometric bead array, formed an in vitro surrogate marker and correlate of the in vivo host immune response toward the scaffold. Decellularized scaffolds were free of major histocompatibility complex class I and II antigens and were found to exert anti-inflammatory and immunosuppressive effects, as evidenced by delayed biodegradation time in vivo; reduced sensitized T-cell proliferative activity in vitro; reduced IL-2, IFN-γ, and raised IL-10 levels in cell-culture supernatants; polarization of the macrophage response in vivo toward an M2 phenotype; and improved survival of donor-derived xenogeneic cells at 2 and 4 wk in vivo. Decellularized scaffolds polarize host responses away from a classical TH1-proinflammatory profile and appear to down-regulate T-cell xeno responses and TH1 effector function by inducing a state of peripheral T-cell hyporesponsiveness. These results have substantial implications for the future clinical application of tissue-engineered therapies.

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“…It would certainly be more globally accepted if the immunogenicity of the larynx were eliminated. We and others have recently developed potential solutions to overcome this issue, using synthetic materials or an acellular human larynx graft by decellularization procedure without compromising the angiogenic, biomechanical, and architectural properties of a native larynx [13][14][15].…”

Section: Upper Airways: Nasopharynx Vocal Cords and Larynxmentioning

confidence: 99%