Development of biodegradation process for Poly(DL-lactic acid) degradation by crude enzyme produced by Actinomadura keratinilytica strain T16-1

Panyachanakul, Titiporn; Sorachart, Bodeesorn; Lumyong, Saisamorn; Lorliam, Wanlapa; Kitpreechavanich, Vichien; Krajangsang, Sukhumaporn

doi:10.1016/j.ejbt.2019.04.005

Cited by 27 publications

(15 citation statements)

References 26 publications

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“…Polylactic acid-degrading ability in microorganisms are not widely distributed as PLA degraders are less discovered than other types of plastic degrading microorganisms (Nishida and Tokiwa, 1993;Pranamuda et al, 1997;Suyama et al, 1998;Tokiwa et al, 2009). Actinobacteria are among the few microorganisms with potential for PLA degradation (Jarerat et al, 2002Sukkhum et al, 2009aSukkhum et al, ,b, 2011Sukkhum et al, , 2012Chomchoei et al, 2011;Konkit et al, 2012;Penkhrue et al, 2015Penkhrue et al, , 2018Panyachanakul et al, 2017Panyachanakul et al, , 2019. PLA-degrading actinobacteria are affiliated with 26 species in 11 genera namely Actinomadura, Amycolatopsis, Kibdelosporangium, Micromonospora, Nonomuraea, Pseudonocardia, Saccharothrix, Streptoalloteichus, Streptomyces, Thermomonospora, and Thermopolyspora ( Table 1).…”

Section: Diversity Of Pla-degrading Actinobacteria: How To Find and Wmentioning

confidence: 99%

“…PDLLA-degrading enzyme production of 766.33 U/mL with 15.97 U/mL•h of enzyme productivity were achieved with the optimum fermentation conditions of 0.25 vvm aeration, 170 rpm agitation and 45 • C incubation temperature for 48 h in batch fermentation using 5L stirrer fermenter. Recently, a scaleup for PLA degrading enzyme production by A. keratinilytica T16-1 in a 5L stirred tank bioreactor was carried out under batch condition (Panyachanakul et al, 2019). The best condition for PLA degradation was an agitation speed of 50 rpm at 60 • C under a controlled pH of 8.0.…”

Section: Development and Application Of Pla Degradation By Actinobactmentioning

confidence: 99%

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Actinobacteria as Promising Candidate for Polylactic Acid Type Bioplastic Degradation

Butbunchu

Pathom-aree

2019

Front. Microbiol.

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Polylactic acid (PLA) is one of the most commercially available and exploited bioplastics worldwide. It is an important renewable polymer for the replacement of petroleumbased plastic materials. They are both biodegradable and bio-based plastic. Microbial degrading activity is a desirable method for environmental safety and economic value for bioplastic waste managements. Members of the phylum actinobacteria are found to play an important role in PLA degradation. Most of the PLA degrading actinobacteria belong to the family Pseudonocardiaceae. Other taxa include members of the family Micromonosporaceae, Streptomycetaceae, Streptosporangiaceae, and Thermomonosporaceae. This mini-review aims to provide an overview on PLA degrading actinobacteria including their diversity and taxonomy, isolation and screening procedures and PLA degrading enzyme production from 1997 to 2019. Consideration is also given to where to sampling and how we might use these beneficial actinobacteria for PLA waste management.

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Section: Diversity Of Pla-degrading Actinobacteria: How To Find and Wmentioning

confidence: 99%

Section: Development and Application Of Pla Degradation By Actinobactmentioning

confidence: 99%

Actinobacteria as Promising Candidate for Polylactic Acid Type Bioplastic Degradation

Butbunchu

Pathom-aree

2019

Front. Microbiol.

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“…Moreover, the shear force of the impeller stimulated the breakdown of bioplastic structures. Panyachanakul et al [22] also found that agitation in the stirrer fermenter positively impacted the degradation of bioplastics. Lomthong et al [14] reported that increasing the agitation rate in the stirrer reactor to 200 rpm accelerated the degradation of PLA polymer (100 g/L) by up to 68 %.…”

Section: Ofmentioning

confidence: 98%

Degradation of Poly(Butylene Succinate) and Poly(Butylene Succinate)/Poly(Lactide) Blends using Serine Protease Produced from Laceyella sacchari LP175

et al. 2021

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The thermophilic filamentous bacterium Laceyella sacchari LP175 was cultivated in a 10.0 L airlift fermenter to produce serine protease at 50 °C. Maximal serine protease activity at 1,123.32 ± 15.8 U/mL was obtained for cultivation at 0.6 vvm aeration rate for 36 h. The crude enzyme was applied for degradation of poly (butylene succinate) (PBS), and poly (butylene succinate)/poly(lactide) blend (PBS/PLA) powders at 50 °C for 48 h with different substrates and enzyme concentrations. Results showed that serine protease produced from L. sacchari LP175 degraded PBS and PBS/PLA at 46.5 ± 2.05 and 49.8 ± 1.45 %, respectively, at an initial substrate concentration of 100 g/L with 1,200 U/mL of serine protease activity. Percentage degradation of PBS and PBS/PLA was improved to 51.4 ± 1.06 and 56.9 ± 1.42 %, respectively, when upscaled in a 2.0 L stirrer fermenter with 200 rpm agitation rate. Degradation products evaluated by a scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) confirmed that serine protease produced from L. sacchari LP175 degraded both PBS and PBS/PLA polymers. Results showed that microbial enzyme technology could be used to degrade PBS and PBS/PLA blend polymers and reduce the accumulation of waste. HIGHLIGHTS Upscaled serine protease production was achieved in a 10 L airlift fermenter by sacchari LP175 using low-cost agricultural products as substrate The crude enzyme degraded PBS and PBS/PLA powders (100 g/L) at up to 51.4 and 56.9 %, respectively in a 2.0 L stirrer fermenter under optimal conditions Degradation products of PBS and PBS/PLA by crude enzyme produced from sacchari LP175 were characterized GRAPHICAL ABSTRACT

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“…Esterase and lipase are important hydrolytic enzymes that can cleave the internal ester bonds of these polyesters. In addition to both of these enzymes, proteases can degrade the members of the poly(α-hydroxy acid) family as a result of the α-ester linkages in their backbone [14]. These degradation products (i.e., PLA, PGA, and PLGA) can enter the Krebs cycle and be eliminated from the body as carbon dioxide (CO 2 ) and water, resulting in the high biocompatibility of aliphatic polyesters [12].…”

Section: Biodegradation and Biocompatibility Of Aliphatic Polyestersmentioning

confidence: 99%

Aliphatic Polyester Nanoparticles for Drug Delivery Systems

Kreua-ongarjnukool¹,

Soomherun²,

Niyomthai³

et al. 2022

Smart Drug Delivery

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Drug delivery systems using aliphatic polyester nanoparticles are usually prepared via an emulsion process. These nanoparticles can control drug release and improve pharmacokinetics. Aliphatic polyesters are linear polymers containing ester linkages, showing sensitivity to hydrolytic degradation. The byproducts then promote autocatalytic degradation. These byproducts could enter the Krebs cycle and be eliminated from the body, resulting in the high biocompatibility of these nanoparticles. The properties of these polyesters are linked to the drug release rate due to biodegradation, i.e., polymer crystallinity, glass transition temperature, polymer hydrophobicity, and molecular weight (MW), all of which relatively influence hydrolysis. Mathematical equations have been used to study the factors and mechanisms that affect drug dissolution compared to experimental release data. The equations used as models for predicting the kinetics of drug release include the zero-order, first-order, Higuchi, Hixson-Crowell, and Korsmeyer-Peppas equations. Aliphatic polyester-based controlled drug delivery has surrounded much of the current activity in the estimation parameters of nanoparticles and stimulated additional research. Polymeric nanoparticles have potential in a wide range of applications, such as in biotechnology, vaccine systems, and the pharmaceutical industry. The main goal of this chapter is to discuss aliphatic polyester nanoparticles as drug carrier systems.

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Development of biodegradation process for Poly(DL-lactic acid) degradation by crude enzyme produced by Actinomadura keratinilytica strain T16-1

Cited by 27 publications

References 26 publications

Actinobacteria as Promising Candidate for Polylactic Acid Type Bioplastic Degradation

Actinobacteria as Promising Candidate for Polylactic Acid Type Bioplastic Degradation

Degradation of Poly(Butylene Succinate) and Poly(Butylene Succinate)/Poly(Lactide) Blends using Serine Protease Produced from Laceyella sacchari LP175

Aliphatic Polyester Nanoparticles for Drug Delivery Systems

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