Development of biocalorimetry as a technique for process monitoring and control in technical scale fermentations

Türker, Mustafa

doi:10.1016/j.tca.2004.01.036

Cited by 54 publications

(38 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yet heat effects in cellular cultures often go unnoticed when one is working with conventional laboratory equipment because most of the heat released by the culture is lost to the environment too quickly to give rise to a perceivable temperature increase. This, however, is completely different in microbial cultures at large scale [5][6][7][8]. As opposed to laboratory reactors, industrial-size fermenters operate nearly adiabatically due to their much smaller surface-to-volume ratio.…”

Section: Why Should We Deal With Heat Dissipation Rates?mentioning

confidence: 99%

See 1 more Smart Citation

Biothermodynamics of live cells: a tool for biotechnology and biochemical engineering

Stockar

2010

Journal of Non-Equilibrium Thermodynamics

View full text Add to dashboard Cite

The aim of this contribution is to review the application of thermodynamics to live cultures of microbial and other cells and to explore to what extent this may be put to practical use. A major focus is on energy dissipation effects in industrially relevant cultures, both in terms of heat and Gibbs energy dissipation. The experimental techniques for calorimetric measurements in live cultures are reviewed and their use for monitoring and control is discussed. A detailed analysis of the dissipation of Gibbs energy in chemotrophic growth shows that it reflects the entropy production by metabolic processes in the cells and thus also the driving force for growth and metabolism. By splitting metabolism conceptually up into catabolism and biosynthesis, it can be shown that this driving force decreases as the growth yield increases. This relationship is demonstrated by using experimental measurements on a variety of microbial strains. On the basis of these data, several literature correlations were tested as tools for biomass yield prediction. The prediction of other culture performance characteristics, including product yields for biorefinery planning, energy yields for biofuel manufacture, maximum growth rates, maintenance requirements, and threshold concentrations is also briefly reviewed.

show abstract

Section: Why Should We Deal With Heat Dissipation Rates?mentioning

confidence: 99%

“…This approach has been demonstrated on a 300 l pilot-scale bioreactor producing biological pesticides in India by Voisard et al [8]. Later Türker [7] applied the method to a 100 m 3 industrial bioreactor.…”

Section: On-line Monitoring and Control Of Bioprocesses By Heat Dissimentioning

confidence: 99%

Biothermodynamics of live cells: a tool for biotechnology and biochemical engineering

Stockar

2010

Journal of Non-Equilibrium Thermodynamics

View full text Add to dashboard Cite

show abstract

“…Microcalorimeters were the first available calorimeters to measure the heat produced by mammalian cells [23,27]. With a high sensitivity they offer the possibility to monitor weak exothermic cultures such as mammalian cell cultures [29]. The microcalorimeters are externally connected to the reactor [23] and continuous samples flow to the calorimetric chamber.…”

Section: Calorimetersmentioning

confidence: 99%

“…Although this heat measuring method is highly sensitive and has shown promising results in measuring the metabolic activity of CHO suspension cultures [23,27], the different environments between the reactor vessel and heat measuring cell can be a major drawback [29,30]. A slight change in pH, aeration, foaming or mixing can affect the cells and their metabolic activity.…”

Section: Calorimetersmentioning

confidence: 99%

The Application of Dielectric Spectroscopy and Biocalorimetry for the Monitoring of Biomass in Immobilized Mammalian Cell Cultures

2015

View full text Add to dashboard Cite

Abstract:The purpose of this study was to introduce dielectric spectroscopy and biocalorimetry as monitoring methods to follow immobilised Chinese Hamster Ovary (CHO) cell culture development. The theory behind both monitoring techniques is explained and perfusion cultures are performed in a Reaction Calorimeter (eRC1 from Mettler Toledo) as an application example. The findings of this work show that dielectric spectroscopy gives highly reliable information upon the viable cell density throughout the entire culture. On the other hand, the RC1 could only provide accurate data from day 5, when the cell density exceeded 4 × 10 , a viable cell density commonly achieved in fed-batch and the early stages of a perfusion culture. This work suggests that biocalorimetry should be possible to implement at industrial scale to monitor CHO cell cultures.

show abstract

“…Theerarattananoon et al [16] reported overall metabolic heat generation 43kJ mol -1 glucose consumed or 32kJ mol -1 ethanol produced during fermentation using S. cerevisiae at 25º C, when initial glucose concentration was kept at 300g l -1 glucose. The heat loss to environment is negligible in the industrial scale bioreactors because these are operated nearly adiabatically due to much lower surface to volume ratio compared to laboratory-scale bioreactors [17], requiring huge quantity of cooling water to maintain optimum temperature. Approximately 60-70% of total chilling water load is used in bioreactors for ethanol production by using mesophiles [18].…”

Section: Introductionmentioning

confidence: 99%

Cooling System Economy in Ethanol Production Using Thermotolerant Yeast Kluyveromyces Sp. IIPE453

Kumar¹,

Dheeran²,

Singh³

et al. 2013

AJMR

View full text Add to dashboard Cite

The growth of thermotolerant/ thermophilic ethanol producing yeast and the fermentation processes using sugary substrates are exothermic processes. If the fermenters are heat insulated, the requirement of heat for maintaining the fermentation broth for ethanol production may be reduced considerably. The heat generated due to growth of thermotolerant yeast Kluyveromyces sp. IIPE453 was found to be 652kJ mol -1 at 50°C using glucose as a substrate. The heat generated due to ethanol formation by Kluyveromyces sp. IIPE453 was found to be 132.54kJ mol -1 of sugar consumed or 67.84kJ mol -1 of ethanol produced at 50°C using sugarcane molasses as substrate. This heat would be sufficient for maintaining the desired temperature, if insulated fermentation systems are used. Therefore, no additional heat would be required to maintain the temperature in fermentation process by thermotolerant yeast at 50°C .

show abstract

Development of biocalorimetry as a technique for process monitoring and control in technical scale fermentations

Cited by 54 publications

References 9 publications

Biothermodynamics of live cells: a tool for biotechnology and biochemical engineering

Biothermodynamics of live cells: a tool for biotechnology and biochemical engineering

The Application of Dielectric Spectroscopy and Biocalorimetry for the Monitoring of Biomass in Immobilized Mammalian Cell Cultures

Cooling System Economy in Ethanol Production Using Thermotolerant Yeast Kluyveromyces Sp. IIPE453

Contact Info

Product

Resources

About