Development of bilayer and trilayer nanofibrous/microfibrous scaffolds for regenerative medicine

Bye, Frazer J.; Bissoli, Julio; Black, Leanne; Bullock, Anthony J.; Puwanun, Sasima; Moharamzadeh, Keyvan; Reilly, Gwendolen C.; Ryan, Anthony J.; MacNeil, Sheila

doi:10.1039/c3bm60074b

Cited by 39 publications

(30 citation statements)

References 55 publications

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“…It is an excellent biomaterial due to its mechanical properties and biocompatibility. Tissue engineering applications include: bone (Bye et al 2013;Phipps et al 2012), cartilage (Steele et al 2014), ligament (Petrigliano et al 2014), cornea (Baradaran-Rafii et al 2016) and vascular (Wise et al 2011) to name a few. Polymer-based scaffolds, such as PCL, have been highlighted as a potential avenue for tissue engineered kidneys (Moon et al 2016), but there is little investigation down this stream.…”

Section: Introductionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream.Focus within kidney tissue engineering has been on 2-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas. The variation in morphology of tissue engineering scaffold has been shown to effect the way cells behave and integrate. In this study we examined the cellular response to scaffold architecture of novel electrospun scaffold for kidney tissue engineering. Fibre diameters of 1.10±0.16 µm and 4.49±0.47 µm were used with 3 distinct scaffold architectures. Traditional random fibres were spun onto a mandrel rotating at 250 rpm, aligned at 1800 rpm with novel cryogenic fibres spun onto a mandrel loaded with dry ice rotating at 250 rpm. Human kidney epithelial cells were grown for 1 and 2 weeks. Fibre morphology had no effect of cell viability in scaffolds with a large fibre diameter but significant differences were seen in smaller fibres. Fibre diameter had a significant effect in aligned and cryogenic scaffold. Imaging detailed the differences in cell attachment due to scaffold differences. These results show that architecture of the scaffold has a profound effect on kidney cells; whether that is effects of fibre diameter on the cell attachment and viability or the effect of fibre arrangement on the distribution of cells and their alignment with fibres. Results demonstrate that PCL scaffolds have the capability to maintain kidney cells life and should be investigated further as a potential scaffold in kidney tissue engineering.

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Section: Introductionmentioning

confidence: 99%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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“…In these early experiments delamination of the nano-porous layer from the micro-porous layer was a potential issue. 18 We have further developed this scaffold so that the nano-porous layer can be interwoven within a PLLA micro-porous scaffold. The PHBV and PLLA fibres co-mingle as a central cell impermeable layer to provide segregation and to prevent delamination.…”

Section: Introductionmentioning

confidence: 99%

Development of a Basement Membrane Substitute Incorporated Into an Electrospun Scaffold for 3D Skin Tissue Engineering

Bye¹,

Bullock²,

Singh³

et al. 2014

j biomater tissue eng

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“…Moreover, modification of fibers using bioactive ceramic particles can lead to the development of new materials for the manufacturing of implants which can establish direct chemical bonds with bone tissue after implantation [27,28]. A nano-fibrous membrane in a bilayer scaffold can also act as a barrier membrane which serves two functions: it is permeable to nutrients yet, when coupled with a micro-fibrous scaffold, allows for the proliferation of cells on both sides of the barrier while at the same time preventing the unwanted migration of cells across the barrier [29].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of bioactive polycaprolactone/hydroxyapatite scaffolds with final bilayer nano-/micro-fibrous structures for tissue engineering application

Rajzer

2014

J Mater Sci

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In this study, two techniques, namely electrospinning and needle-punching processes, were used to fabricate bioactive polycaprolactone/hydroxyapatite scaffolds with a final bilayer nano-/micro-fibrous porous structure. A hybrid scaffold was fabricated to combine the beneficial properties of nanofibers and microfibers and to create a three-dimensional porous structure (which is usually very difficult to produce using electrospinning technology only). The first part of this work focused on determining the conditions necessary to fabricate nano-and micro-fibrous components of scaffold layers. A characterization of scaffold components, with respect to their morphology, fiber diameter, pore size, wettability, chemical composition and mechanical properties, was performed. Then, the same process parameters were applied to produce a hybrid bilayer scaffold by electrospinning the nanofibers directly onto the micro-fibrous nonwovens obtained in a traditional mechanical needle-punching process. In the second part, the bioactive character of a hybrid nano-/ micro-fibrous scaffold in simulated body fluid (SBF) was assessed. Spherical calcium phosphate was precipitated onto the nano-/micro-fibrous scaffold surface proving its bioactivity.

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Development of bilayer and trilayer nanofibrous/microfibrous scaffolds for regenerative medicine

Cited by 39 publications

References 55 publications

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

Development of a Basement Membrane Substitute Incorporated Into an Electrospun Scaffold for 3D Skin Tissue Engineering

Fabrication of bioactive polycaprolactone/hydroxyapatite scaffolds with final bilayer nano-/micro-fibrous structures for tissue engineering application

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