Development of backcross recombinant inbred lines between Oryza sativa Nipponbare and O. rufipogon and QTL detection on drought tolerance

Abstract

Backcross populations at advanced generations are useful in evaluating the agronomic traits from unadapted germplasms, such as wild species. In this study, we generated 159 backcross recombinant inbred lines (BRILs) at the BC 2 F 8 generation between Oryza sativa Nipponbare (a recurrent parent) and O. rufipogon W630 (a donor parent). These BRILs were found to have nearly homozygous genome constitutions based on the marker genotypes at 180 SSR loci covering whole chromosomes. Since their selfed progenies have almost identical genotypes in each line, they were further used for QTL analysis of drought tolerance. Out of six QTLs detected, three were found to have wild alleles with favorable effects on drought tolerance. The present BRILs with known marker data can be applied to more QTL analyses of traits specific to wild species.

Identification of Quantitative Trait Loci Controlling Floral Morphology of Rice Using a Backcross Population between Common Cultivated Rice, <i>Oryza sativa</i> and Asian Wild Rice, <i>O. rufipogon</i>

“…The wild accession was kindly provided by the National Institute of Genetics, Japan. A single wild plant of O. rufipogon W630 was crossed twice with O. sativa Nipponbare as previously described [17], and by the single-seed-descendant method, 159 BRILs were produced at the BC 2 F 8 generation [18]. These BRILs theoretically contain 12.5% of the wild genome in the genetic background of O. sativa Nipponbare.…”

“…The genotypes for 159 BRILs at 180 polymorphic simple sequence repeat (SSR) marker loci between Nipponbare and W630 were previously obtained [18]. With these marker data and the phenotypic data obtained in the present study, QTL analysis for eight floral and two grain traits was carried out by composite interval mapping using WinQTL Cartographer ver.…”

“…To understand the genetic basis of the differences in eight floral traits between wild and cultivated rice, we used 159 BRILs previously developed [18]. Each line was genetically fixed and had theoretically 12.5% of the wild genome in the genetic background of Nipponbare.…”

Identification of QTL for Striga hermonthica Resistance Using Backcross Population Derived from a Cross between Oryza sativa (cv. Nipponbare) and O. rufipogon

“…Estimation of wild chromosomal segments in each of the 159 BRILs at BC2F8 generation ranged between 0.0 to 23.6% (Thanh et al, 2011). The estimation was done using 180 microsatellite loci covering 1,362 cM of the 12 rice chromosomes.…”

Magnaporthe oryzae Genetic Diversity and Its Outcomes on the Search for Durable Resistance

“…These four species are known for its traits related to tolerance/resistance to biotic and abiotic stress (Thanh et al, 2011;Nonomura et al, 2010;Vaughan et al, 2005, Rangel et al, 2006. In the 2010/2011 growing season, when evaluating the F3 generation, segregant populations originated from crosses between O. glaberrima and SCS 112 presented good agronomic traits and a productivity of more that 10 t/ha.…”