Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue Culture

Abstract

Bioreactors are growing in popularity among network engineers. Bioreactors are generally chambers used for cell culture processes with predetermined parameters. Pivot is a bioreactor system for cell culture that is controlled automatically and remotely using multichambers that allow inflow of fresh liquid or output for sample collection. This system facilitates gas exchange between the culture medium and ambient gas. The system provides a storage bag for fresh media and a peristaltic pump is used to move the media in a closed loop during perfusion and stirring in the cell culture chamber. The flow rates used is 2 mL/min. The system has a container for direct sample measurement to obtain pH, oxygen, and carbon dioxide parameters. The bioreactor system is also equipped with a mixing vessel which allows the addition of desired nutrients or additives to the system. The results of a computational fluid dynamics (CFD) and shear stress-based flow analysis have been conducted. The simulation results demonstrate that the applied Pivot parameters correspond to an ideal environment to hepatocyte cell viability and growth. This research is expected to increase the number of cells produced without reducing the quality of each chamber and be carried out simultaneously, automatically, and remotely controlled.

Design of Specific and Efficient sgRNA for CRISPR/Cas9 System to Knockout Superoxide Dismutase 2 in Breast Cancer Stem Cells

“…Tissue engineering is currently playing a significant role in the rapid progression of various technologies in medical applications (Irsyad et al, 2022). The integration of gene editing strategies into tissue engineering, necessitating nuclease modifications is essential 354 Design of Specific and Efficient sgRNA for CRISPR/Cas9 System to Knockout Superoxide Dismutase 2 in Breast Cancer Stem Cells in the treatment of numerous genetic diseases.…”