Development of Atom Transfer Radical Polymer-Modified Gold Nanoparticle-Based Enzyme-Linked Immunosorbent Assay (ELISA)

Abstract

In this work, a novel enzyme-linked immunosorbent assay (ELISA) with a low limit of detection and high sensitivity was developed using atom transfer radical polymer (ATRP)-modified gold nanoparticles (AuNPs). Clear signal amplification was achieved by introducing an abundance of horseradish peroxidase (HRP) to the AuNPs, because of the ATRP modification. This result suggested that the new ELISA was able to detect antigens in complex mixtures, and the limit of detection (LOD) was lower than that of conventional ELISA by a factor of 81. The new ELISA strategy greatly decreased the LOD during analysis and exhibited excellent reproducibility, stability, and feasibility. Therefore, it is a promising technique with many potential applications in biochemistry and medical science research.

A Sensitive and Simple Enzyme-Linked Immunosorbent Assay Using Polymer as Carrier

“…22,23) Polymers have many advantages over other reaction carriers, such as their abundant functional groups, numerous branched chains and large internal voids. 24,25) In addition, the polymer used in this study has no effect on the activity of biological molecules.…”

Interfacial Assembly of Signal Amplified Multienzymes and Biorecognized Antibody into Proteinosome for an Ultrasensitive Immunoassay

“…For example, magnetic beads were employed as a scaffold to loading a large amount of acetylcholinesterase (AChE) and antibody for amplifying signal . Following, horseradish peroxidase (HRP) was covalently conjugated to polymer modified gold nanoparticles for establishment of immunoassay whose sensitivity was 81‐fold higher than conventional ELISA . Gu et al further increased the loading amount of HRP by utilizing a nanospherical poly (acrylic acid) brushes as carriers, enhancing the detection sensitivity .…”

Enzyme-controlled dissolution of MnO2 nanoflakes with enzyme cascade amplification for colorimetric immunoassay

“…Enzyme-based labelling strategies are usually used during this measurement (Berg et al, 2015;Geng et al, 2015;Qu et al, 2014). For example, horseradish peroxidase (HRP) could catalyze 3,3',5,5'-tetramethylbenzidine (TMB) for development of blue color in the presence of hydrogen peroxide (H 2 O 2 ) (Chen et al, 2014). However, the detection sensitive was always limited due to the limitation of the labelling amount on each antibody.…”