Development of arginine vasotocin innervation in two species of anuran amphibian:Rana catesbeiana andRana sylvatica

Wb, Mathieson

doi:10.1007/bf01463933

Cited by 20 publications

(16 citation statements)

References 31 publications

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“…In accordance with the present gene expression data, VT production is detected from the premetamorphic period in many hypothalamic and extra-hypothalamic neurons, such as preoptic magnocellular neurons which project mainly to the neurohypophysis, neurons in the lateral septum, the amygdala, the preoptic area and the ventral hypothalamus (Mathieson 1996). This early appearance of VT-producing neurons and VT innervation in the brain suggests that VT has neuroregulatory functions beginning at the premetamorphic period.…”

Section: Controlsupporting

confidence: 90%

Expression of the vasotocin gene in the hypothalamus of intact and osmotically stimulated bullfrogs during metamorphosis

Hyodo

1999

Journal of Molecular Endocrinology

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To study the ontogeny of the vasotocin (VT) system and its contribution to anuran metamorphosis, VT mRNA levels were determined by Northern blot analysis in metamorphosing bullfrog tadpoles. Effects of osmotic stimulation on VT mRNA levels were also analyzed in order to follow the development of osmotic responsiveness of VT neurons. The intensity of hybridization signals for VT mRNA gradually increased during prometamorphic development. The increase became marked thereafter until metamorphic climax. Plasma osmolality and hematocrit remained unchanged before metamorphosis, and increased after metamorphic climax, indicating that climactic tadpoles in a semiterrestrial environment were in a dehydrated condition. These increases correlated well with the increase in VT mRNA level. Immersion of tadpoles in 30% seawater (approximately 350 mOsmol) for 3 days increased plasma osmolality at all stages. No significant changes were observed in the VT mRNA level in response to this treatment during premetamorphic stages. The VT mRNA levels were significantly higher in the treated tadpoles after preclimax stages. Hyperosmotic treatment also increased hematocrit until early metamorphic climax, but did not alter it in tadpoles at late metamorphic climax. These results suggest that the responsiveness of VT-producing neurons to hyperosmotic or hypovolemic stimulation, or both, is established by the time of the metamorphic climax in bullfrog. The marked increase in VT mRNA levels at metamorphic climax stages of intact individuals is probably induced by dehydration. VT-stimulated water absorption and reabsorption in the target organs probably prevented the increase in hematocrit at late metamorphic climax. Thus VT may contribute importantly to osmoregulatory mechanisms in relation to adaptation to a semiterrestrial habitat through the metamorphosis.

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Section: Controlsupporting

confidence: 90%

Expression of the vasotocin gene in the hypothalamus of intact and osmotically stimulated bullfrogs during metamorphosis

Hyodo

1999

Journal of Molecular Endocrinology

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“…First, no AVT-ir cells were found in the septal nucleus of T. natans. Septal AVT-ir cells have been found in the bullfrog (R. catesbeiana) brain Mathieson, 1996) but not in any other amphibian described so far. Second, suprachiasmatic AVT-ir cells also were not found in this caecilian.…”

Section: Discussionmentioning

confidence: 92%

“…Furthermore, neurosecretory cells have been identified in this region in the brains of two other caecilians (Chthonerpeton indistinctum, Ichthyophis paucisulcus) by using classic histological and enzyme histochemical techniques (Welsch et al, 1976). Anuran and urodele amphibians also possess hypothalamic AVT cells (e.g., R. catesbeiana, R. ridibunda, R. sylvatica, X. laevis, and P. waltlii;Gonzalez and Smeets, 1992a,b;Mathieson, 1996). This population is more variable in other vertebrates, however.…”

Section: Discussionmentioning

confidence: 98%

“…Distribution of immunoreactive AVT and MT has been well described for eight anurans (Rana temporaria, R. esculenta, R. ridibunda, R. catesbeiana, R. sylvatica, Bufo bufo, B. japonicus, and Xenopus laevis;Vandesande and Dierickx, 1976;Urano, 1985, 1987;Conway and Gainer, 1987;Gonzalez and Smeets, 1992a, b;Boyd, 1994a;Mathieson, 1996) and for one urodele amphibian (Pleurodeles waltlii; Gonzalez and Smeets, 1992a). The distribution of AVT and MT has never been described in any caecilian species.…”

Section: Indexing Terms: Vasotocin; Mesotocin; Vasopressin; High-perfmentioning

confidence: 98%

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Identification and localization of neurohypophysial peptides in the brain of a caecilian amphibian,Typhlonectes natans (amphibia: Gymnophiona)

1998

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The amphibian order Gymnophiona contains more than 150 different species of caecilians. The characterization and distribution of neurohypophysial peptides, however, has not been described for any member of this order. By using high-performance liquid chromatography, radioimmunoassay, and mass spectrometry, we identified the peptide arginine vasotocin (AVT) in brain and pituitary extracts from the caecilian Typhlonectes natans. By using immunocytochemistry, we found five populations of AVT-immunoreactive (AVT-ir) cells in the brain of T. natans. AVT-ir cell bodies were located in the preoptic area, amygdala pars medialis, ventral thalamus, dorsal hypothalamic nucleus, and nucleus of the solitary tract. AVT-ir fibers and terminal fields were widespread. We also identified a mesotocin-like peptide. The distribution of this peptide in the brain of T. natans was more restricted than the distribution of AVT. Mesotocin-like-immunoreactive cell bodies were located almost exclusively in the preoptic area, with only a few other cells located in the amygdala pars medialis. This caecilian species, therefore, possesses neurohypophysial peptides that are similar in their structure and distribution to the peptides found in anuran and urodele amphibian orders.

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“…Previous studies of amphibians have identified VTir neuronal perikarya within the caudal isthmic region of P. waltlii (Gonzá lez and Smeets, 1992a) and R. catesbeiana Boyd, 1994;Mathieson, 1996). The VTir cells in P. waltlii are believed to be located slightly lateral 64 C.A.…”

Section: Locus Coeruleus Nucleus Of the Solitary Tract And Superiormentioning

confidence: 95%

Neuroanatomical distribution of vasotocin in a urodele amphibian (Taricha granulosa) revealed by immunohistochemical and in situ hybridization techniques

et al. 1997

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Immunohistochemical and in situ hybridization techniques were used to investigate the neuroanatomical distribution of arginine vasotocin-like systems in the roughskin newt (Taricha granulosa). Vasotocin-like-immunoreactive neuronal cell bodies were identified that, based on topographical position, most likely, are homologous to groups of vasopressin-immunoreactive neuronal cell bodies described in mammals, including those in the bed nucleus of the stria terminalis, medial amygdala, basal septal region, magnocellular basal forebrain-including the horizontal limb of the diagonal band of Broca, paraventricular and supraoptic nuclei, suprachiasmatic nucleus, and dorsomedial hypothalamic nucleus. Several additional vasotocin-like-immunoreactive cell groups were observed in the forebrain and brainstem regions; these observations are compared with previous studies of vasotocin- and vasopressin-like systems in vertebrates. Arginine vasotocin-like-immunoreactive fibers and presumed terminals also were widely distributed with high densities in the basal limbic forebrain, the ventral preoptic and hypothalamic regions, and the brainstem ventromedial tegmentum. Based on in situ hybridization studies with synthetic oligonucleotide probes for vasotocin and the related neuropeptide mesotocin, as well as double-labeling studies with combined immunohistochemistry and in situ hybridization, we conclude that the vasotocin immunohistochemical procedures used identify vasotocin-like, but not mesotocin-like, elements in the brain of T. granulosa. The distribution of arginine vasotocin-like systems in T. granulosa is greater than the distribution previously reported for any other single vertebrate species; however, it is consistent with an emerging pattern of distribution of vasotocin- and vasopressin-like peptides in vertebrates. Complexity in the vasotocinergic system adds further support to the conclusion that this peptide regulates multiple neurophysiological and neuroendocrinological functions.

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Development of arginine vasotocin innervation in two species of anuran amphibian:Rana catesbeiana andRana sylvatica

Cited by 20 publications

References 31 publications

Expression of the vasotocin gene in the hypothalamus of intact and osmotically stimulated bullfrogs during metamorphosis

Expression of the vasotocin gene in the hypothalamus of intact and osmotically stimulated bullfrogs during metamorphosis

Identification and localization of neurohypophysial peptides in the brain of a caecilian amphibian,Typhlonectes natans (amphibia: Gymnophiona)

Neuroanatomical distribution of vasotocin in a urodele amphibian (Taricha granulosa) revealed by immunohistochemical and in situ hybridization techniques

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