2016
DOI: 10.1021/acs.analchem.6b02709
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Development of APTES-Decorated HepG2 Cancer Stem Cell Membrane Chromatography for Screening Active Components from Salvia miltiorrhiza

Abstract: Cell membrane chromatography (CMC) is an ideal method for screening potential active components acting on target cell membranes from a complex system, such as herbal medicines. But due to the decay and falling-off of membranes, the CMC column suffers from short life span and low reproducibility. This has greatly limited the application of this model, especially when the cell materials are hard to obtain. To solve this problem, a novel type of (3-aminopropyl)triethoxysilane (APTES)-decorated silica gel was empl… Show more

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Cited by 60 publications
(41 citation statements)
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“…To characterize and identify active components interacting with the cell membrane stationary phase, several 2D LC methods have been developed for the rapid investigation of complex TCMs, including heart‐cutting 2D CMC coupled with MS and a comprehensive 2D CMC–MS method . The comprehensive 2D LC–MS system is a powerful analytical technique because of its rapid, efficient separation, high resolution, and powerful identification abilities when coupled with MS, and it is suitable for the analysis of complex TCMs .…”
Section: Introductionmentioning
confidence: 99%
“…To characterize and identify active components interacting with the cell membrane stationary phase, several 2D LC methods have been developed for the rapid investigation of complex TCMs, including heart‐cutting 2D CMC coupled with MS and a comprehensive 2D CMC–MS method . The comprehensive 2D LC–MS system is a powerful analytical technique because of its rapid, efficient separation, high resolution, and powerful identification abilities when coupled with MS, and it is suitable for the analysis of complex TCMs .…”
Section: Introductionmentioning
confidence: 99%
“…APTES was modified on the surface of silica, which can provide an amino group to covalently link cell membrane fragments with the help of glutaraldehyde. Ding et al (2016) utilized APTES‐decorated silica gel, which reacts with the amino groups on cell membranes to form a covalent bond. The CMC column life was extended from 3 to 12 days.…”
Section: Ba‐ms Relying On Cell Membrane Chromatographymentioning
confidence: 99%
“…Nonetheless, the CMC suffers from the short life span and low reproducibility, which is caused by the decay and falling‐off of membranes . Ding et al . developed a novel type of (3‐aminopropyl) triethoxysilane (APTES)‐decorated silica gel, which can react with the amino groups on cell membranes to form a covalent bond for preventing the falling‐off of membranes.…”
Section: Two‐dimensional (2d) Lc–msmentioning
confidence: 99%