Development of antithrombotic peptides based on the molecular interactions between von Willebrand factor and GPIbα

Abstract

Binding of platelets on vascular endothelia at the damaged site using von Willebrand factor (vWF) as a bridge is of great significance for platelet adhesion and subsequent arterial thrombosis. Molecular...

Design and characterization of antithrombotic ClEKnsTy-Au nanoparticles as diagnostic and therapeutic reagents

“…The binding free energy was calculated by the protocol that used in the literature 37,38 and our previous work. 16,18,19,39–41 The equations were described briefly as follows.Δ G bind = Δ G polar + Δ G nonpolar Δ G polar = Δ G elec + Δ G PB Δ G nonpolar = Δ G vdW + Δ G SASA …”

“…The binding free energy was calculated by the protocol that used in the literature 37,38 and our previous work. 16,18,19,[39][40][41] The equations were described briefly as follows.…”

“…The binding of AuNP-Cl on collagen was verified by an enzymelinked immunosorbent assay (ELISA). 41,48 100 mL of ClEKnsTy solution (1 mg mL À1 ) was added to 15 mL of AuNP solution, reacted under stirring conditions for 1 h in the dark, centrifuged at 15 000g for 20 min at 4 1C, and a total of 300 mL of the purple-red oily precipitate at the bottom of the centrifuge tube was taken as the AuNP-Cl sample. 300 mL of AuNP sample was prepared under the same conditions for use.…”